YKL162C

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Systematic name	YKL162C
Gene name
Aliases
Feature type	ORF, Uncharacterized
Coordinates	Chr XI:148838..147630
Primary SGDID	S000001645

Description of YKL162C: Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion




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Community Commentary

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Acids

UW-Stout/Acids_SP23

Analysis

These are the results we gathered from testing a pH of # against the control wild yeast and the gene YKL162C. The three red lines are the growth rates of the BY (wild yeast) mixed with a pH of # and the three blue lines are the three growth rates of the YKL162C knockout gene mixed with a pH of #. All trials demonstrated strong cell growth, the YKL162C gene did grow at a more rapid pace. The average double time of the knockout gene was 19.3 minutes and the average double time of the wild yeast was 13.57 minutes. Overall, all trials showed good cell growth and the knockout surpassed the wild yeast in growth of the 18 hour period.

Caffeine

UW-Stout/Caffeine SP23

Analysis

Glucose Sensitivity

Protocol

[1]

Analysis

The graph displayed above shows the results of a viability assay of a YKL162C knockout strain as compared to BY4735 (wild type) and other knockout strains. This knockout strain was found to have overall more cell death than all other strains assayed. With the YKL162C gene removed nearly all cells died when incubated for 48 hours in 2% Glucose. This indicates a higher sensitivity to glucose than was exemplified in the wild type strain. There appears to be a significant associated death rate in glucose solution for this strain as compared to BY4735. Perhaps, YKL162C is of more biological importance in the BY4735 wild type than previously thought. Further research should consider why this knockout strain had complete glucose-induced apoptosis, as well as higher overall cell death rate, as compared to the other strains tested in this study.

Isopropanol

UW-Stout/Isopropanol_SP23

Analysis

These results were what was expected and all 3 were consistent throughout the trials. Although there was less growth than the control group, it could mean that the cell is more sensitive to the isopropanol with the gene knocked out. In the third trial there was a little bit of spiking towards the end around 15:20-18:00 but other than that it was a good growth curve.

Heat Shock

UW-Stout/Heat_shock_SP23

YKL162 Yeast Colonies at 40 degrees Celsius

Time (sec)	Colonies
0	50
15	41
45	25
75	24
105	13
135	25

Wild Type Yeast Colonies at 40 degrees Celsius

Time (sec)	Colonies
0	24
15	20
45	17
75	16
105	18
135	14

Analysis

The general trend of our data yielded a negative relationship between time of heat shock. About half of the yeast cells died off in a matter of 135 seconds at 40 degrees Celsius. The YKL162C yeast were dying off at a faster rate over time compared to the Wild Yeast strain. This means that the gene that was knocked out in the YKL162C yeast could have cause some resistance to heat shock.

Bases

Analysis

As you can see there was clearly an error with the A11 sample of yeast 3. The other two samples showed less growth than the Wild Type yeast, A1 A2 A3, over the 24 hour time period. This knockout gene likely did not aid in basic pH environmental growth.

Protocol- UW-Stout/Bases SP23

References

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