Difference between revisions of "YLR006C"
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==Heat Shock== | ==Heat Shock== | ||
{| class="wikitable" | {| class="wikitable" | ||
| − | |+ | + | |+ SSK1 Yeast Colonies at 40 degrees Celsius |
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! Time (sec) !! Colonies !! | ! Time (sec) !! Colonies !! | ||
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| 0 || 24 || | | 0 || 24 || | ||
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| − | | 15 || | + | | 15 || 20 || |
|- | |- | ||
| − | | 45 || | + | | 45 || 17 || |
|- | |- | ||
| − | | 75 || | + | | 75 || 16 || |
|- | |- | ||
| − | | 105 || | + | | 105 || 18 || |
|- | |- | ||
| − | | 135 || | + | | 135 || 14 || |
|} | |} | ||
[[Image:DKATrial2Yeast.jpg]] | [[Image:DKATrial2Yeast.jpg]] | ||
Revision as of 11:31, 2 May 2023
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| Systematic name | YLR006C |
| Gene name | SSK1 |
| Aliases | |
| Feature type | ORF, Verified |
| Coordinates | Chr XII:163893..161755 |
| Primary SGDID | S000003996 |
Description of YLR006C: Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate[1][2][3][4]
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Contents
Community Commentary
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Protein Details
Protein Modification
Modification(s): Phosphorylation
Identified as an efficient substrate of Clb2-Cdk1-as1 in a screen of a proteomic GST-fusion library. [4] [5]
Isopropanol
Analysis
These results were what was expected with only a little bit of difference. The first trial and third trials were close and there was less growth than the control which could have been because the knockout genes were more sensitive to the isopropanol. The second trial gave different results in which there was more growth than the control which is the opposite of the first and third. This could have been caused by an error because there was consistency in the other two.
Heat Shock
| Time (sec) | Colonies | |
|---|---|---|
| 0 | 24 | |
| 15 | 14 | |
| 45 | 8 | |
| 75 | 9 | |
| 105 | 10 | |
| 135 | 15 |
| Time (sec) | Colonies | |
|---|---|---|
| 0 | 24 | |
| 15 | 20 | |
| 45 | 17 | |
| 75 | 16 | |
| 105 | 18 | |
| 135 | 14 |
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Bases
References
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- ↑ Mao K, et al. (2011) Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae. J Cell Biol 193(4):755-67 SGD PMID 21576396
- ↑ Posas F, et al. (1996) Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 two-component osmosensor. Cell 86(6):865-75 SGD PMID 8808622
- ↑ Sato N, et al. (2003) Phosphorelay-regulated degradation of the yeast Ssk1p response regulator by the ubiquitin-proteasome system. Mol Cell Biol 23(18):6662-71 SGD PMID 12944490
- ↑ 4.0 4.1 Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
SGD PMID 14574415 Cite error: Invalid
<ref>tag; name "S000074306" defined multiple times with different content - ↑ submitted by Jeff Ubersax on 2004-01-29
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