Difference between revisions of "UW-Stout/Hydrogen Peroxide SP22"
(→Calibration Protocol) |
(→Calibration Protocol) |
||
| Line 30: | Line 30: | ||
| − | Trial 1 | + | ===Trial 1 |
Percent Series: | Percent Series: | ||
| − | 3%, 1%, 0.3%, 0.1%, 0.03%, 0.01% | + | 3%, 1%, 0.3%, 0.1%, 0.03%, 0.01%=== |
| Line 39: | Line 39: | ||
| − | Trial 2 | + | ===Trial 2 |
Percent Series; | Percent Series; | ||
| − | 3%, 2%, 1.33%, 0.88%, 0.59%, 0.40% | + | 3%, 2%, 1.33%, 0.88%, 0.59%, 0.40%=== |
[[File:2_2trial.jpg]] | [[File:2_2trial.jpg]] | ||
Revision as of 11:41, 3 May 2022
Introduction
Hydrogen Peroxide (H2O2) is a very strong oxidizer and antiseptic that is often used in dilute forms. The overall compound is found in biological systems but is in short lived reactions due to it's high toxicity over time. The goal of our experiment was to test how multiple yeast strains with knockout genes would respond in the presence of varying concentrations of hydrogen peroxide.
Materials
- Hydrogen Peroxide Solution 3% Concentration
- Distilled water for dilutions
- Wild Type and Knockout Yeast Strains
Equipment
- P-200 Micropipette
- P-20 Micropipette
- P-10 Micropipette
- Centrifuge Tubes
- Yeast Test Well
Calibration Protocol
H2O2 Dilutions
- Vortex Yeast Strains
- Create 50ul Hydrogen Peroxide Dilutions
- Combine Dilutions With 50ul yeast cultures
- Put Individual Wells to Growth For 24 Hours
===Trial 1
Percent Series:
3%, 1%, 0.3%, 0.1%, 0.03%, 0.01%===
===Trial 2 Percent Series; 3%, 2%, 1.33%, 0.88%, 0.59%, 0.40%===
