Difference between revisions of "YBL086C"
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The protocol can be found at | The protocol can be found at | ||
===[[UW-Stout/Caffeine_FA19|Caffeine]]=== | ===[[UW-Stout/Caffeine_FA19|Caffeine]]=== | ||
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+ | [[File:yBL086cUV_Exposure.png]] | ||
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+ | This bar graph Shows that compared to a BY4735 background, Knocking out yBL086c seems to make the yeast more sensitive to UV light exposure. | ||
<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation --> | <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation --> |
Revision as of 10:54, 12 December 2019
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Systematic name | YBL086C |
Gene name | |
Aliases | |
Feature type | ORF, Uncharacterized |
Coordinates | Chr II:62602..61202 |
Primary SGDID | S000000182 |
Description of YBL086C: Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery[1]
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Community Commentary
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Growth Curve
In a BY4735 background, knocking out YBL086C seems to have little or no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YAL061 knock-out strain's doubling time was 495 minutes. (These doubling times are the means of three experiments.)
Caffeine and Yeast Cells
This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 743.32 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.
The protocol can be found at
Caffeine
This bar graph Shows that compared to a BY4735 background, Knocking out yBL086c seems to make the yeast more sensitive to UV light exposure.
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References
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