Difference between revisions of "UW Stout/Ethanol FA19"
(Created page with "Yeast torture 1 protocol: Materials: # neat ethanol # yeast # sterile water # 200 ul # 96 well plate Procedure: # (add 50ul mixture to 50ul yeast in 96 well plate.)x2. Used...") |
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− | Yeast torture 1 | + | ==Yeast torture 1== |
+ | ===Protocol:=== | ||
Materials: | Materials: | ||
# neat ethanol | # neat ethanol | ||
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| 50 | | 50 | ||
| 50 | | 50 | ||
+ | |} | ||
+ | ==Knockout Strand Yeast Torture=== | ||
+ | ===Protocol:=== | ||
+ | Materials: | ||
+ | *Yeast Knockout Strains: | ||
+ | ##YAL061W | ||
+ | ##YBL086C | ||
+ | ##YBL9081W | ||
+ | ##YBR033W | ||
+ | ##YBR138C | ||
+ | ##YBR220C | ||
+ | ##YHR048W | ||
+ | ##YGL140C | ||
+ | ##YJR061W | ||
+ | ##YKR005C | ||
+ | ##WT:BY4735 | ||
+ | {| class="wikitable" | ||
+ | ! yeast | ||
+ | ! 1 | ||
+ | ! 2 | ||
+ | ! 3 | ||
+ | ! 4 | ||
+ | ! 5 | ||
+ | ! 6 | ||
+ | ! 7 | ||
+ | ! 8 | ||
+ | ! 9 | ||
+ | ! 10 | ||
+ | ! 11 | ||
+ | ! 12 | ||
+ | |- | ||
+ | | ul yeast | ||
+ | | 50 | ||
+ | | 50 | ||
+ | | 50 | ||
+ | | 50 | ||
+ | | 50 | ||
+ | | 50 | ||
+ | | 50 | ||
+ | | 50 | ||
+ | | 50 | ||
+ | | 50 | ||
+ | | 50 | ||
+ | | 50 | ||
+ | |- | ||
+ | | ul ethanol | ||
+ | | 7 | ||
+ | | 7 | ||
+ | | 7 | ||
+ | | 7 | ||
+ | | 7 | ||
+ | | 7 | ||
+ | | 7 | ||
+ | | 7 | ||
+ | | 7 | ||
+ | | 7 | ||
+ | | 7 | ||
+ | | 7 | ||
+ | |- | ||
+ | | ul water | ||
+ | | 43 | ||
+ | | 43 | ||
+ | | 43 | ||
+ | | 43 | ||
+ | | 43 | ||
+ | | 43 | ||
+ | | 43 | ||
+ | | 43 | ||
+ | | 43 | ||
+ | | 43 | ||
+ | | 43 | ||
+ | | 43 | ||
|} | |} |
Revision as of 10:42, 10 December 2019
Yeast torture 1
Protocol:
Materials:
- neat ethanol
- yeast
- sterile water
- 200 ul
- 96 well plate
Procedure:
- (add 50ul mixture to 50ul yeast in 96 well plate.)x2. Used 12 wells in total
- fill 6 200ul tubes, each with water and ethanol concentration x2. Resulting in 6 200ul tubes with 100ul of mixture in each.
1 | 2 | 3 | 4 | 5 | 6 | |
---|---|---|---|---|---|---|
ul ethanol | 0 | 3.75 | 7 | 10.25 | 13.5 | 16.75 |
ul water | 50 | 46.25 | 43 | 39.75 | 37.5 | 33.25 |
ul yeast | 50 | 50 | 50 | 50 | 50 | 50 |
Knockout Strand Yeast Torture=
Protocol:
Materials:
- Yeast Knockout Strains:
- YAL061W
- YBL086C
- YBL9081W
- YBR033W
- YBR138C
- YBR220C
- YHR048W
- YGL140C
- YJR061W
- YKR005C
- WT:BY4735
yeast | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
---|---|---|---|---|---|---|---|---|---|---|---|---|
ul yeast | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 |
ul ethanol | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 |
ul water | 43 | 43 | 43 | 43 | 43 | 43 | 43 | 43 | 43 | 43 | 43 | 43 |