Difference between revisions of "Commonly used auxotrophic markers"
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| − | !|Allele!!Deleted ORF?!!Reverts?!!Molecular Description<sup>a</sup>!!Reference | + | !|Allele!!Deleted ORF?!!Reverts?!!Notes!!Molecular Description<sup>a</sup>!!Reference |
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| + | [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=ade1 ade1-14] | ||
| + | | | ||
| + | no | ||
| + | | | ||
| + | yes | ||
| + | | | ||
| + | red colonies | ||
| + | | | ||
| + | TGG-to-TGA nonsense change at codon 244; | ||
| + | GGA-to-GAA missense change at codon 185. | ||
| + | | | ||
| + | [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000060944 Nakayashiki et al. 2001] | ||
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Revision as of 10:02, 8 March 2012
This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in Brachmann et al. (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you have additions or modifications you would like to suggest.
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| Allele | Deleted ORF? | Reverts? | Notes | Molecular Descriptiona | Reference |
|---|---|---|---|---|---|
|
no |
yes |
red colonies |
TGG-to-TGA nonsense change at codon 244; GGA-to-GAA missense change at codon 185. |
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| edit table |
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aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.
bAll trp- strains are cold sensitive (Singh and Manney 1974).
