Difference between revisions of "Commonly used auxotrophic markers"
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<sup>a</sup>The sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1. | <sup>a</sup>The sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1. | ||
| − | <sup>b</sup>All trp- strains are cold sensitive (Singh and Manney 1974). | + | <sup>b</sup>All trp- strains are cold sensitive ([http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000057172 Singh and Manney 1974]). |
Revision as of 09:57, 8 March 2012
This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in Brachmann et al. (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you have additions or modifications you would like to suggest.
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| Allele | Deleted ORF? | Reverts? | Molecular Descriptiona | Reference |
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| edit table |
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aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.
bAll trp- strains are cold sensitive (Singh and Manney 1974).
