Difference between revisions of "YCL063W"
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+ | === Alleles, Strains, and Phenotypes === | ||
+ | [[Category:Topic:Alleles, Strains, and Phenotypes]] | ||
+ | ==== Partial Deletion ==== | ||
+ | [[Category:Topic:Alleles, Strains, and Phenotypes:Partial Deletion]] | ||
+ | '''Strain Background''': LWY 7235<br> | ||
+ | '''Genotype''': LWY 5798 (vac17::TRP1)<br> | ||
+ | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]], Recessive [[Category:Phenotype:Recessive]], Viable [[Category:Phenotype:Viable]] | ||
+ | '''Mutation type(s)''': Partial deletion from 17364 to partialDelTo | ||
+ | |||
+ | In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | === Interactions === | ||
+ | [[Category:Topic:Interactions]] | ||
+ | ==== Physical ==== | ||
+ | [[Category:Topic:Interactions:Physical]] | ||
+ | Physical interaction with VAC8 [[Category:Physical Interactions with VAC8]]<br> | ||
+ | Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | Physical interaction with MYO2 [[Category:Physical Interactions with MYO2]]<br> | ||
+ | Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | ==== Two Hybrid ==== | ||
+ | [[Category:Topic:Interactions:Two Hybrid]] | ||
+ | Two Hybrid interaction with VAC8 [[Category:Two Hybrid Interactions with VAC8]]<br> | ||
+ | Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | === Protein Details === | ||
+ | [[Category:Topic:Protein Details]] | ||
+ | ==== Protein Degradation ==== | ||
+ | [[Category:Topic:Protein Details:Protein Degradation]] | ||
+ | |||
+ | Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | ==== Protein Function/Process ==== | ||
+ | [[Category:Topic:Protein Details:Protein Function/Process]] | ||
+ | |||
+ | Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | ==== Protein Localization ==== | ||
+ | [[Category:Topic:Protein Details:Protein Localization]] | ||
+ | |||
+ | Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
__TOC__ | __TOC__ | ||
==Community Commentary== | ==Community Commentary== | ||
{{CommentaryHelp}} | {{CommentaryHelp}} | ||
+ | === Alleles, Strains, and Phenotypes === | ||
+ | [[Category:Topic:Alleles, Strains, and Phenotypes]] | ||
+ | ==== Partial Deletion ==== | ||
+ | [[Category:Topic:Alleles, Strains, and Phenotypes:Partial Deletion]] | ||
+ | |||
+ | '''Strain Background''': LWY 7235<br> | ||
+ | '''Genotype''': LWY 5798 (vac17::TRP1)<br> | ||
+ | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]], Recessive [[Category:Phenotype:Recessive]], Viable [[Category:Phenotype:Viable]] | ||
+ | '''Mutation type(s)''': Partial deletion from 17364 to partialDelTo | ||
+ | |||
+ | In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | === Interactions === | ||
+ | [[Category:Topic:Interactions]] | ||
+ | ==== Physical ==== | ||
+ | [[Category:Topic:Interactions:Physical]] | ||
+ | Physical interaction with VAC8 [[Category:Physical Interactions with VAC8]]<br> | ||
+ | Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | Physical interaction with MYO2 [[Category:Physical Interactions with MYO2]]<br> | ||
+ | Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | ==== Two Hybrid ==== | ||
+ | [[Category:Topic:Interactions:Two Hybrid]] | ||
+ | Two Hybrid interaction with VAC8 [[Category:Two Hybrid Interactions with VAC8]]<br> | ||
+ | Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | === Protein Details === | ||
+ | [[Category:Topic:Protein Details]] | ||
+ | ==== Protein Degradation ==== | ||
+ | [[Category:Topic:Protein Details:Protein Degradation]] | ||
+ | |||
+ | Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | ==== Protein Function/Process ==== | ||
+ | [[Category:Topic:Protein Details:Protein Function/Process]] | ||
+ | |||
+ | Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | |||
+ | {{ShortCenteredHR}} | ||
+ | ==== Protein Localization ==== | ||
+ | [[Category:Topic:Protein Details:Protein Localization]] | ||
+ | |||
+ | Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref> | ||
+ | {{ShortCenteredHR}} | ||
==References== | ==References== | ||
<!-- REFERENCES ARE AUTOMATICALLY GENERATED. PLEASE DON'T EDIT THIS SECTION--> | <!-- REFERENCES ARE AUTOMATICALLY GENERATED. PLEASE DON'T EDIT THIS SECTION--> | ||
{{RefHelp}} | {{RefHelp}} |
Revision as of 17:05, 23 January 2007
Share your knowledge...Edit this entry!
Systematic name | YCL063W | |
Gene name | VAC17 | |
Aliases | YCL062W | |
Feature type | ORF, Verified | |
Coordinates | Chr III:17290..18561 | |
Don't edit this box! It's automatically regenerated, and edits will be lost when the update script runs. |
Description of YCL063W: Protein involved in vacuole inheritance; acts as a vacuole-specific receptor for myosin Myo2p[1][2]
Alleles, Strains, and Phenotypes
Partial Deletion
Strain Background: LWY 7235
Genotype: LWY 5798 (vac17::TRP1)
Phenotype(s): Loss of function (Null), Recessive, Viable
Mutation type(s): Partial deletion from 17364 to partialDelTo
In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. [2] [3]
Interactions
Physical
Physical interaction with VAC8
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. [2] [3]
Physical interaction with MYO2
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. [2] [3]
Two Hybrid
Two Hybrid interaction with VAC8
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. [2] [3]
Protein Details
Protein Degradation
Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. [2] [3]
Protein Function/Process
Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. [2] [3]
Protein Localization
Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. [2] [3]
Contents
Community Commentary
About Community Commentary. Please share your knowledge!
Alleles, Strains, and Phenotypes
Partial Deletion
Strain Background: LWY 7235
Genotype: LWY 5798 (vac17::TRP1)
Phenotype(s): Loss of function (Null), Recessive, Viable
Mutation type(s): Partial deletion from 17364 to partialDelTo
In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. [2] [3]
Interactions
Physical
Physical interaction with VAC8
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. [2] [3]
Physical interaction with MYO2
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. [2] [3]
Two Hybrid
Two Hybrid interaction with VAC8
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. [2] [3]
Protein Details
Protein Degradation
Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. [2] [3]
Protein Function/Process
Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. [2] [3]
Protein Localization
Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. [2] [3]
References
See Help:References on how to add references
- ↑ Ishikawa K, et al. (2003) Identification of an organelle-specific myosin V receptor. J Cell Biol 160(6):887-97 SGD PMID 12642614
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92
SGD PMID 12594460 Cite error: Invalid
<ref>
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tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>
tag; name "S000072665" defined multiple times with different content - ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 submitted by Fusheng Tang on 2003-03-17
See Help:Categories on how to add the wiki page for this gene to a Category
- Pages with reference errors
- ORF
- ORF, Verified
- Topic:Alleles, Strains, and Phenotypes
- Topic:Alleles, Strains, and Phenotypes:Partial Deletion
- Phenotype:Loss of function (Null)
- Phenotype:Recessive
- Phenotype:Viable
- Topic:Interactions
- Topic:Interactions:Physical
- Physical Interactions with VAC8
- Physical Interactions with MYO2
- Topic:Interactions:Two Hybrid
- Two Hybrid Interactions with VAC8
- Topic:Protein Details
- Topic:Protein Details:Protein Degradation
- Topic:Protein Details:Protein Function/Process
- Topic:Protein Details:Protein Localization