Difference between revisions of "UW-Stout/Ethanol FA21"
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#*3.2% =193 ul to 6.4 ul of ethanol | #*3.2% =193 ul to 6.4 ul of ethanol | ||
#*1% = 198 ul to 2 ul of ethanol | #*1% = 198 ul to 2 ul of ethanol | ||
− | #Using the | + | #Using the wild type yeast place into well plates with 50ul of yeast solution and 50ul of diluted ethanol |
==Wield type of yeast== | ==Wield type of yeast== | ||
− | [[Pre- | + | [[Image:Pre-knockout.jpg|Pre-knockout|400px]] |
+ | |||
+ | ==Yeast Knockout strain== | ||
+ | ===Procedure=== | ||
+ | #Dilute the ethanol with the equations below | ||
+ | #Making the ethanol | ||
+ | #*18.5% =163 ul of H20 to 37 ul of ethanol | ||
+ | #Using the five different types of yast | ||
+ | #*BY- Wild type yeast | ||
+ | #*1-YHL012W | ||
+ | #*2-YMR221C | ||
+ | #*3-YPR078C | ||
+ | #*4-YBR184W | ||
+ | #*5-YBR284W | ||
+ | [[Image:Yeast Torture.jpg|Yeast Torture|400px]] |
Latest revision as of 16:24, 20 December 2021
Contents
Materials & Equipment
- Ethanol 100%
- Sterile Water
- Well plates
- Pipets
- Pipet Tips
- Vent Hood
- Centrifuge
- Willed type yeast
Safety
- Eye protection
- Latex gloves
- Lab coat
- Refer to the SDS (safety data sheet) for ethanol
Procedures
- Dilute the ethanol with the equations below
- Making the ethanol
- 50% = 100 ul of H2O to 100 ul of ethanol
- 33.3% =133.4 ul of H2O to 66.6 ul of ethanol
- 22.2% =155.5 ul of H2O to 44.4 ul of ethanol
- 18.5% =163 ul of H20 to 37 ul of ethanol
- 15.4% = 169.2 ul of H2O to 20.8ul of ethanol
- 12.8% = 174 ul to 25.6ul of ethanol
- 10% = 180 ul to 20ul of ethanol
- 8.8% = 182.4 ul to 17.6ul of ethanol
- 7.4% = 185.2 ul to 14.8ul of ethanol
- 4.9% = 190 ul to 9.6ul of ethanol
- 3.2% =193 ul to 6.4 ul of ethanol
- 1% = 198 ul to 2 ul of ethanol
- Using the wild type yeast place into well plates with 50ul of yeast solution and 50ul of diluted ethanol
Wield type of yeast
Yeast Knockout strain
Procedure
- Dilute the ethanol with the equations below
- Making the ethanol
- 18.5% =163 ul of H20 to 37 ul of ethanol
- Using the five different types of yast
- BY- Wild type yeast
- 1-YHL012W
- 2-YMR221C
- 3-YPR078C
- 4-YBR184W
- 5-YBR284W