Difference between revisions of "Commonly used auxotrophic markers"
(Created page with "Commonly Used Auxotrophic Markers This table describes some commonly used auxotrophic markers (along with some novel useful markers); it was obtained from Brachmann et al. (1998...") |
|||
(27 intermediate revisions by 4 users not shown) | |||
Line 1: | Line 1: | ||
− | + | This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in [https://www.yeastgenome.org/reference/S000041186 Brachmann et al.] (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you would like to suggest additions or modifications. | |
− | |||
− | + | {| border="1" class="wikitable" | |
+ | |- | ||
+ | !|Allele!!Deleted ORF?!!Reverts?!!Notes!!Molecular Description<sup>a</sup>!!Reference | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/ade1 <em>ade1-14</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | red colonies | ||
+ | | | ||
+ | TGG-to-TGA nonsense change at codon 244; | ||
+ | GGA-to-GAA missense change at codon 185 | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000060944 Nakayashiki et al. 2001] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/ade1 <em>ade1-101</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
− | + | | | |
− | + | simple point mutation causing a requirement for adenine | |
− | + | | | |
− | ade2-1 | + | |
+ | | | ||
+ | [http://www.yeastgenome.org/reference/395030 Botstein et al. 1979] | ||
+ | |- | ||
+ | |||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/ade2 <em>ade2-1</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | ochre mutation | ||
+ | | | ||
+ | TTA-to-TTG silent change at codon 9, | ||
GAA-to-TAA ochre nonsense change at codon 64, | GAA-to-TAA ochre nonsense change at codon 64, | ||
AGA-to-GGA missense change at codon 101, | AGA-to-GGA missense change at codon 101, | ||
GTT-to-GTC silent change at codon 124, | GTT-to-GTC silent change at codon 124, | ||
− | ACG-to-ACA silent change at codon 539 | + | ACG-to-ACA silent change at codon 539 |
− | ade2-101 | + | | |
− | ade2-BglII | + | Rodney Rothstein, [http://wiki.yeastgenome.org/index.php/CommunityW303.html Personal communication to SGD] |
− | can1-100 | + | |- |
− | + | | | |
− | Note that this deletion damages the PET56 promoter. See Zhang et al. | + | [http://www.yeastgenome.org/locus/ade2 <em>ade2-101</em>] |
− | + | | | |
− | his3-11,15 | + | no |
− | G deletion at nucleotide 319 | + | | |
− | leu2delta1 | + | yes |
− | leu2-3,112 | + | | |
− | GTT-to-GCT | + | ochre mutation, red colonies |
+ | | | ||
+ | G to T transversion at nucleotide 190, changing codon 64 from a Glu to a Stop | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000087056 Gai and Voytas, 2005] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/ade2 <em>ade2-BglII</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | red colonies | ||
+ | | | ||
+ | frameshift (BglII site filled in at position 592) | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000049198 Engebrecht and Roeder 1990] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/can1 <em>can1-100</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | ochre mutation | ||
+ | | | ||
+ | AAA-to-TAA ochre nonsense change at codon 47 | ||
+ | | | ||
+ | Rodney Rothstein, [http://wiki.yeastgenome.org/index.php/CommunityW303.html Personal communication to SGD] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/his3 <em>his3delta200</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | Cold sensitive; high frequency of petite formation, especially during transformation. Note that this deletion damages the PET56 promoter. See [https://www.yeastgenome.org/reference/S000074003 Zhang et al. (2003)] for a discussion of this issue. | ||
+ | | | ||
+ | 1 kb deletion (-205 to 835) | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000042846 Struhl 1985]; [https://www.yeastgenome.org/reference/S000046453 Fasullo and Davis 1988] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/his3 <em>his3delta1</em>] | ||
+ | | | ||
+ | partial | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | chemically constructed deletion; reversion is undetectable (< 10(E-9) revertants in a culture). Causes histidine to be required for growth; allows selection of vectors carrying the HIS3 gene. | ||
+ | | | ||
+ | 187 bp HindIII-HindIII internal deletion (305 to 492) | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000040818 Scherer and Davis 1979] | ||
+ | [http://www.yeastgenome.org/reference/395030 Botstein et al. 1979] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/his3 <em>his3-11,15</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | double mutant | ||
+ | | | ||
+ | G deletion at nucleotide 208, | ||
+ | G deletion at nucleotide 319 | ||
+ | | | ||
+ | Rodney Rothstein, [http://wiki.yeastgenome.org/index.php/CommunityW303.html Personal communication to SGD] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/his3 <em>his3delta</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | In the S288C background, the his3 null mutation confers sensitivity to acetic acid. | ||
+ | | | ||
+ | complete deletion | ||
+ | | | ||
+ | [http://www.yeastgenome.org/reference/23828602 Ding et al. 2013] | ||
+ | |- | ||
+ | |||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/leu2 <em>leu2delta1</em>] | ||
+ | | | ||
+ | partial | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | |||
+ | | | ||
+ | EcoRI-ClaI internal deletion (163 to 649, 0.6 kb) | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000044428 Sikorski and Hieter 1989] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/leu2 <em>leu2-3,112</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | double mutant; leu2-3 and leu2-112 are each frameshift mutations; the double mutants revert very infrequently (less than 10(E-8) revertants in a culture) and cause leucine to be required for growth. This allows the selection of vectors carrying the LEU2 gene. | ||
+ | | | ||
+ | GTC-to-GTT silent change at codon 56, | ||
+ | GTT-to-GCT missense change at codon 69, | ||
G insertion at nucleotide 249, | G insertion at nucleotide 249, | ||
G insertion at nucleotide 792, | G insertion at nucleotide 792, | ||
GTT-to-GTC silent change at codon 299, | GTT-to-GTC silent change at codon 299, | ||
− | GAC-to-AAC missense change at codon 300. | + | GAC-to-AAC missense change at codon 300 |
− | Gaber and Culbertson 1982; | + | | |
− | Meira LB et al., 1995; | + | [https://www.yeastgenome.org/reference/S000043265 Hinnen et al. 1978]; |
− | Rodney Rothstein, Personal communication to SGD. | + | [https://www.yeastgenome.org/reference/S000042541 Gaber and Culbertson 1982]; |
− | lys2-801 | + | [https://www.yeastgenome.org/reference/S000069760 Meira LB et al., 1995]; |
− | lys2delta202 | + | [http://wiki.yeastgenome.org/index.php/CommunityW303.html Rodney Rothstein, Personal communication to SGD] |
− | trp1delta1 | + | [http://www.yeastgenome.org/reference/395030 Botstein et al. 1979] |
− | trp1delta63 | + | |- |
− | trp1-1 | + | | |
− | trp1- | + | [http://www.yeastgenome.org/locus/leu2 <em>leu2delta</em>] |
− | ura3-52 | + | | |
− | ura3-1 | + | yes |
− | The | + | | |
− | ade2delta::hisG | + | no |
− | leu2delta0 | + | | |
− | lys2delta0 | + | In the S288C background, the leu2 null mutation confers sensitivity to acetic acid. |
− | met15delta0 | + | | |
− | ura3delta0 | + | complete deletion |
− | + | | | |
+ | [http://www.yeastgenome.org/reference/23828602 Ding et al. 2013] | ||
+ | |- | ||
+ | |||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/lys2 <em>lys2-801</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | amber mutation | ||
+ | | | ||
+ | |||
+ | | | ||
+ | |||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/lys2 <em>lys2delta202</em>] | ||
+ | | | ||
+ | partial | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | |||
+ | | | ||
+ | XhoI-HpaI internal deletion (1813 to 2864, 1.0 kb) | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000047446 Winston et al. 1995] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/lys2 <em>lys2delta</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | In the S288C background, the lys2 null mutation confers sensitivity to acetic acid. | ||
+ | | | ||
+ | complete deletion | ||
+ | | | ||
+ | [http://www.yeastgenome.org/reference/23828602 Ding et al. 2013] | ||
+ | |- | ||
+ | |||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/trp1 <em>trp1delta1</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | cold sensitive<sup>b</sup>, weak galactose inducer (deletes <em>GAL3</em> UAS), removes <em>ARS1</em>, also called <em>trp1-901</em> | ||
+ | | | ||
+ | 1.45 kb deletion, EcoRI-EcoRI (-102 to 1352) | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000044428 Sikorski and Hieter 1989] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/trp1 <em>trp1delta63</em>] | ||
+ | | | ||
+ | partial | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | cold sensitive<sup>b</sup> | ||
+ | | | ||
+ | 0.6 kb deletion, EcoRI-HindIII (-102 to 513) | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000044428 Sikorski and Hieter 1989] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/trp1 <em>trp1-1</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | amber mutation | ||
+ | | | ||
+ | GAG-to-TAG amber nonsense change at codon 83 | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000041995 McDonald et al. 1997] | ||
+ | |- | ||
+ | |||
+ | |||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/trp1 <em>trp1-1</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | cold sensitive<sup>b</sup> | ||
+ | | | ||
+ | C to T at residue 403 of the coding sequence, changing residue 135 from glutamine to an amber stop codon | ||
+ | | | ||
+ | Brian Green and Joachim Li, unpublished results | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/trp1 <em>trp1delta</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | In the S288C background, the trp1 null mutation confers sensitivity to acetic acid. | ||
+ | | | ||
+ | complete deletion | ||
+ | | | ||
+ | [http://www.yeastgenome.org/reference/23828602 Ding et al. 2013] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/trp1 <em>trp1-289</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | Reverts at low frequency (about 10(E-7) revertants in a culture) and causes tryptophan to be required for growth. | ||
+ | | | ||
+ | Allows the selection of vectors carrying the TRP1 gene. | ||
+ | | | ||
+ | [http://www.yeastgenome.org/reference/395030 Botstein et al. 1979] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/trp5 <em>trp5delta</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | In the S288C background, the trp5 null mutation confers sensitivity to acetic acid. | ||
+ | | | ||
+ | complete deletion | ||
+ | | | ||
+ | [http://www.yeastgenome.org/reference/23828602 Ding et al. 2013] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/ura3 <em>ura3-52</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | |||
+ | | | ||
+ | Ty1 insertion (transcribing left to right) at pos. 121 | ||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000043686 Rose and Winston 1984] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/ura3 <em>ura3-1</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | |||
+ | | | ||
+ | G to A transition at residue 701 of the coding sequence, changing residue 234 from glycine to glutamate | ||
+ | | | ||
+ | Yan Li, Glenn Manthey, and Adam Bailis, unpublished results | ||
+ | |||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/ura3 <em>ura3delta</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | In the S288C background, the ura3 null mutation confers sensitivity to acetic acid. | ||
+ | | | ||
+ | complete deletion | ||
+ | | | ||
+ | [http://www.yeastgenome.org/reference/23828602 Ding et al. 2013] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/ura4 <em>ura4delta</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | In the S288C background, the ura4 null mutation confers sensitivity to acetic acid. | ||
+ | | | ||
+ | complete deletion | ||
+ | | | ||
+ | [http://www.yeastgenome.org/reference/23828602 Ding et al. 2013] | ||
+ | |- | ||
+ | |||
+ | | | ||
+ | |} | ||
+ | |||
+ | The alleles listed below are described in [https://www.yeastgenome.org/reference/S000041186 Brachmann et al. 1998]. | ||
+ | |||
+ | |||
+ | {| | ||
+ | |- | ||
+ | !|Allele!!Deleted ORF?!!Reverts?!!Notes !!Molecular Description<sup>a</sup>!!Reference | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/ade2 <em>ade2delta::hisG</em>] | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | |||
+ | | | ||
+ | |||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000048958 (Aparicio et al. 1991)] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/leu2 <em>leu2delta0</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | designer deletion | ||
+ | | | ||
+ | |||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000041186 Brachmann et al. 1998] | ||
+ | |- | ||
+ | |||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/lys2 <em>lys2delta0</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | designer deletion | ||
+ | | | ||
+ | |||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000041186 Brachmann et al. 1998] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/met15 <em>met15delta0</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | designer deletion | ||
+ | | | ||
+ | |||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000041186 Brachmann et al. 1998] | ||
+ | |- | ||
+ | | | ||
+ | [http://www.yeastgenome.org/locus/ura3 <em>ura3delta0</em>] | ||
+ | | | ||
+ | yes | ||
+ | | | ||
+ | no | ||
+ | | | ||
+ | designer deletion | ||
+ | | | ||
+ | |||
+ | | | ||
+ | [https://www.yeastgenome.org/reference/S000041186 Brachmann et al. 1998] | ||
+ | |||
+ | |- | ||
+ | | | ||
+ | |} | ||
+ | |||
+ | |||
+ | |||
+ | <sup>a</sup>The sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1. | ||
− | + | <sup>b</sup>All trp- strains are cold sensitive ([https://www.yeastgenome.org/reference/S000057172 Singh and Manney 1974]). |
Latest revision as of 12:34, 11 July 2019
This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in Brachmann et al. (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you would like to suggest additions or modifications.
Allele | Deleted ORF? | Reverts? | Notes | Molecular Descriptiona | Reference |
---|---|---|---|---|---|
no |
yes |
red colonies |
TGG-to-TGA nonsense change at codon 244; GGA-to-GAA missense change at codon 185 |
||
no |
simple point mutation causing a requirement for adenine |
||||
no |
yes |
ochre mutation |
TTA-to-TTG silent change at codon 9, GAA-to-TAA ochre nonsense change at codon 64, AGA-to-GGA missense change at codon 101, GTT-to-GTC silent change at codon 124, ACG-to-ACA silent change at codon 539 |
Rodney Rothstein, Personal communication to SGD | |
no |
yes |
ochre mutation, red colonies |
G to T transversion at nucleotide 190, changing codon 64 from a Glu to a Stop |
||
no |
no |
red colonies |
frameshift (BglII site filled in at position 592) |
||
no |
yes |
ochre mutation |
AAA-to-TAA ochre nonsense change at codon 47 |
Rodney Rothstein, Personal communication to SGD | |
yes |
no |
Cold sensitive; high frequency of petite formation, especially during transformation. Note that this deletion damages the PET56 promoter. See Zhang et al. (2003) for a discussion of this issue. |
1 kb deletion (-205 to 835) |
||
partial |
no |
chemically constructed deletion; reversion is undetectable (< 10(E-9) revertants in a culture). Causes histidine to be required for growth; allows selection of vectors carrying the HIS3 gene. |
187 bp HindIII-HindIII internal deletion (305 to 492) |
||
no |
no |
double mutant |
G deletion at nucleotide 208, G deletion at nucleotide 319 |
Rodney Rothstein, Personal communication to SGD | |
yes |
no |
In the S288C background, the his3 null mutation confers sensitivity to acetic acid. |
complete deletion |
||
partial |
no |
EcoRI-ClaI internal deletion (163 to 649, 0.6 kb) |
|||
no |
no |
double mutant; leu2-3 and leu2-112 are each frameshift mutations; the double mutants revert very infrequently (less than 10(E-8) revertants in a culture) and cause leucine to be required for growth. This allows the selection of vectors carrying the LEU2 gene. |
GTC-to-GTT silent change at codon 56, GTT-to-GCT missense change at codon 69, G insertion at nucleotide 249, G insertion at nucleotide 792, GTT-to-GTC silent change at codon 299, GAC-to-AAC missense change at codon 300 |
Hinnen et al. 1978; Gaber and Culbertson 1982; Meira LB et al., 1995; Rodney Rothstein, Personal communication to SGD Botstein et al. 1979 | |
yes |
no |
In the S288C background, the leu2 null mutation confers sensitivity to acetic acid. |
complete deletion |
||
no |
yes |
amber mutation |
|||
partial |
no |
XhoI-HpaI internal deletion (1813 to 2864, 1.0 kb) |
|||
yes |
no |
In the S288C background, the lys2 null mutation confers sensitivity to acetic acid. |
complete deletion |
||
yes |
no |
cold sensitiveb, weak galactose inducer (deletes GAL3 UAS), removes ARS1, also called trp1-901 |
1.45 kb deletion, EcoRI-EcoRI (-102 to 1352) |
||
partial |
no |
cold sensitiveb |
0.6 kb deletion, EcoRI-HindIII (-102 to 513) |
||
no |
yes |
amber mutation |
GAG-to-TAG amber nonsense change at codon 83 |
||
no |
yes |
cold sensitiveb |
C to T at residue 403 of the coding sequence, changing residue 135 from glutamine to an amber stop codon |
Brian Green and Joachim Li, unpublished results | |
yes |
no |
In the S288C background, the trp1 null mutation confers sensitivity to acetic acid. |
complete deletion |
||
no |
yes |
Reverts at low frequency (about 10(E-7) revertants in a culture) and causes tryptophan to be required for growth. |
Allows the selection of vectors carrying the TRP1 gene. |
||
yes |
no |
In the S288C background, the trp5 null mutation confers sensitivity to acetic acid. |
complete deletion |
||
no |
no |
Ty1 insertion (transcribing left to right) at pos. 121 |
|||
no |
yes |
G to A transition at residue 701 of the coding sequence, changing residue 234 from glycine to glutamate |
Yan Li, Glenn Manthey, and Adam Bailis, unpublished results | ||
yes |
no |
In the S288C background, the ura3 null mutation confers sensitivity to acetic acid. |
complete deletion |
||
yes |
no |
In the S288C background, the ura4 null mutation confers sensitivity to acetic acid. |
complete deletion |
||
The alleles listed below are described in Brachmann et al. 1998.
Allele | Deleted ORF? | Reverts? | Notes | Molecular Descriptiona | Reference |
---|---|---|---|---|---|
no |
no |
||||
yes |
no |
designer deletion |
|||
yes |
no |
designer deletion |
|||
yes |
no |
designer deletion |
|||
yes |
no |
designer deletion |
|||
aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.
bAll trp- strains are cold sensitive (Singh and Manney 1974).