Difference between revisions of "YKL162C"
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The general trend of our data yielded a negative relationship between time of heat shock. About half of the yeast cells died off in a matter of 135 seconds at 40 degrees Celsius. It can be inferred that with a longer amount of time we the yeast cells could continue being killed off until all of them died. | The general trend of our data yielded a negative relationship between time of heat shock. About half of the yeast cells died off in a matter of 135 seconds at 40 degrees Celsius. It can be inferred that with a longer amount of time we the yeast cells could continue being killed off until all of them died. | ||
Revision as of 11:44, 2 May 2023
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| Systematic name | YKL162C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr XI:148838..147630 |
| Primary SGDID | S000001645 |
Description of YKL162C: Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion[1]
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Community Commentary
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Isopropanol
Analysis
These results were what was expected and all 3 were consistent throughout the trials. Although there was less growth than the control group, it could mean that the cell is more sensitive to the isopropanol with the gene knocked out. In the third trial there was a little bit of spiking towards the end around 15:20-18:00 but other than that it was a good growth curve.
Heat Shock
| Time (sec) | Colonies | |
|---|---|---|
| 0 | 50 | |
| 15 | 41 | |
| 45 | 25 | |
| 75 | 24 | |
| 105 | 13 | |
| 135 | 25 |
| Time (sec) | Colonies | |
|---|---|---|
| 0 | 24 | |
| 15 | 20 | |
| 45 | 17 | |
| 75 | 16 | |
| 105 | 18 | |
| 135 | 14 |
The general trend of our data yielded a negative relationship between time of heat shock. About half of the yeast cells died off in a matter of 135 seconds at 40 degrees Celsius. It can be inferred that with a longer amount of time we the yeast cells could continue being killed off until all of them died.
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Bases
References
See Help:References on how to add references
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