Difference between revisions of "YCR095C"

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===Analysis/Conclusion===
 
===Analysis/Conclusion===

Revision as of 15:25, 16 December 2022

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Systematic name YCR095C
Gene name OCA4
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr III:289258..288170
Primary SGDID S000000691


Description of YCR095C: Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts[1][2]




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Ethanol

Caffeine

Control

Trial 1

Trial 2

Interpretation

The average doubling time for the YCR095C (C6) gene in standard conditions was 99 minutes. Under caffeine stress, the average doubling time was 344 minutes. That is a 237% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.



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References

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  1. Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
  2. Kushner DB, et al. (2003) Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus. Proc Natl Acad Sci U S A 100(26):15764-9 SGD PMID 14671320

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UW Stout/FA 2022 Glycerol Media

Introduction

Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.

Results

Knockout 5 Trial 1.png Negative Control KO5 Trial 1.png Knockout 5 Trial 2.png

Analysis/Conclusion