Difference between revisions of "UW-Stout/Ethanol FA22"
| Line 13: | Line 13: | ||
===Procedure=== | ===Procedure=== | ||
| − | #Item 1 | + | #Item 1 Make the ethanol solution by add 942µl of water and 58µl of 100% ethanol |
| − | + | #Item 2 Collect well a clear flat-bottom assay plate | |
| − | 2 | + | #Item 3 Vortex yeast culture for about 5 seconds |
| − | 3 | + | #Item 4 Put 50µl of the yeast culture into all 12 wells. |
| − | 4 | + | #Item 5 In the 1st well add 1µl of your ethanol solution and 49µl of H2O |
| − | 5 | + | #Item 6 In the 2nd well add 2µl of your ethanol solution and 48µl of H2O |
| − | 6 | + | #Item 7 In the 3rd well add 3µl of your ethanol solution and 47µl of H2O |
| − | 7 | + | #Item 8 In the 4th well add 4µl of your ethanol solution and 46µl of H2O |
| − | 8 | + | #Item 9 In the 5th well add 5µl of your ethanol solution and 45µl of H2O |
| − | 9 | + | #Item 10 In the 6th well add 8µl of your ethanol solution and 42µl of H2O |
| − | 10 | + | #Item 11 In the 7th well add 10µl of your ethanol solution and 40µl of H2O |
| − | 11 | + | #Item 12 In the 8th well add 20µl of your ethanol solution and 30µl of H2O |
| − | 12 | + | #Item 13 In the 9th well add 27µl of your ethanol solution and 23µl of H2O |
| − | 13 | + | #Item 14 In the 10th well add 35µl of your ethanol solution and 15µl of H2O |
| − | 14 | + | #Item 15 In the 11th well add 41µl of your ethanol solution and 9µl of H2O |
| − | 15 | + | #Item 16 In the 12th well add 50µl of your ethanol solution and 0µl of H2O |
| − | 16 | + | #Item 17 In E column 12th well add 0µl of your ethanol solution and 50µl of H2O |
| − | 17 | + | #Item 18 Put your well clear flat-bottom assay plate into the incubator and let sit. |
| − | 18 | + | #Item 19 Wait and observe the growth or death |
| − | 19 | ||
Revision as of 12:37, 6 December 2022
Ethanol Yeast Experiment 2022
Introduction
We will add a sample of ethanol solution to a yeast culture and measure the growth afterward. This experiment is to determine what amounts of ethanol solution are best for the tress experiment on our class knockout genes.
Materials
• Corning COSTAR 96-well clear flat-bottom assay plate • Wild-type yeast in 2x synthetic complete media at an OD600 of 0.1-0.2 • Ethanol concertation of 1M (labeled ETH01M)
Procedure
- Item 1 Make the ethanol solution by add 942µl of water and 58µl of 100% ethanol
- Item 2 Collect well a clear flat-bottom assay plate
- Item 3 Vortex yeast culture for about 5 seconds
- Item 4 Put 50µl of the yeast culture into all 12 wells.
- Item 5 In the 1st well add 1µl of your ethanol solution and 49µl of H2O
- Item 6 In the 2nd well add 2µl of your ethanol solution and 48µl of H2O
- Item 7 In the 3rd well add 3µl of your ethanol solution and 47µl of H2O
- Item 8 In the 4th well add 4µl of your ethanol solution and 46µl of H2O
- Item 9 In the 5th well add 5µl of your ethanol solution and 45µl of H2O
- Item 10 In the 6th well add 8µl of your ethanol solution and 42µl of H2O
- Item 11 In the 7th well add 10µl of your ethanol solution and 40µl of H2O
- Item 12 In the 8th well add 20µl of your ethanol solution and 30µl of H2O
- Item 13 In the 9th well add 27µl of your ethanol solution and 23µl of H2O
- Item 14 In the 10th well add 35µl of your ethanol solution and 15µl of H2O
- Item 15 In the 11th well add 41µl of your ethanol solution and 9µl of H2O
- Item 16 In the 12th well add 50µl of your ethanol solution and 0µl of H2O
- Item 17 In E column 12th well add 0µl of your ethanol solution and 50µl of H2O
- Item 18 Put your well clear flat-bottom assay plate into the incubator and let sit.
- Item 19 Wait and observe the growth or death
