Difference between revisions of "UW-Stout/Hydrogen Peroxide SP22"

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(Equipment)
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==Introduction==
 
==Introduction==
 
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Hydrogen Peroxide (H2O2) is a very strong oxidizer and antiseptic that is often used in dilute forms. The overall compound is found in biological systems but is in short lived reactions due to it's high toxicity over time. The goal of our experiment was to test how multiple yeast strains with knockout genes would respond in the presence of varying concentrations of hydrogen peroxide.
 
 
  
  
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*Wild Type and Knockout Yeast Strains
 
*Wild Type and Knockout Yeast Strains
  
*96 Well Plate
 
 
*10 PCR tubes containing Hydrogen Peroxide, Wild Type and Knockout Yeast Strains
 
 
*Sterile Water
 
  
 
==Equipment==
 
==Equipment==
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*P-20 Micropipette
 
*P-20 Micropipette
 
*P-10 Micropipette
 
*P-10 Micropipette
*Micropipette tips
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*Centrifuge Tubes
*Safety gloves
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*Yeast Test Well
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==Calibration Protocol==

Revision as of 11:03, 3 May 2022

Introduction

Hydrogen Peroxide (H2O2) is a very strong oxidizer and antiseptic that is often used in dilute forms. The overall compound is found in biological systems but is in short lived reactions due to it's high toxicity over time. The goal of our experiment was to test how multiple yeast strains with knockout genes would respond in the presence of varying concentrations of hydrogen peroxide.


Materials

  • Hydrogen Peroxide Solution 3% Concentration
  • Distilled water for dilutions
  • Wild Type and Knockout Yeast Strains


Equipment

  • P-200 Micropipette
  • P-20 Micropipette
  • P-10 Micropipette
  • Centrifuge Tubes
  • Yeast Test Well

Calibration Protocol