Difference between revisions of "YDR343C"

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=== Alleles, Strains, and Phenotypes ===
 
[[Category:Topic:Alleles, Strains, and Phenotypes]]
 
==== Multiple Knockout Strains ====
 
[[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
 
 
'''Strain Background''': CEN.PK2-1C<br>
 
'''Genotype''': EBY4000 (hxt)<br>
 
'''Together with''': HXT15, MPH2, HXT7, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11<br>
 
'''Phenotype(s)''': Viable [[Category:Phenotype:Viable]]
 
, Conditionally inviable when on glucose medium
 
 
The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. <ref name='S000056317'>Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 {{SGDpaper|S000056317}} PMID 10618490</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
 
 
{{ShortCenteredHR}}
 
=== DNA and RNA Details ===
 
[[Category:Topic:DNA and RNA Details]]
 
==== Other DNA and RNA Details ====
 
[[Category:Topic:DNA and RNA Details:Other DNA and RNA Details]]
 
'''Other Topic''': expression [[Category:Topic:expression]]
 
 
Specifically lower expression in sulfur limited chemostat cultures versus sulfur excess. <ref name='S000073646'>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 {{SGDpaper|S000073646}} PMID 12414795</ref> <ref name = 'CAset9153-2003-07-25'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=9153 Viktor Boer] on 2003-07-25</ref>
 
 
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==Community Commentary==
 
==Community Commentary==

Revision as of 10:51, 24 January 2007

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Systematic name YDR343C
Gene name HXT6
Aliases
Feature type ORF, Verified
Coordinates Chr IV:1161314..1159602
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Description of YDR343C: High-affinity glucose transporter of the major facilitator superfamily, nearly identical to Hxt7p, expressed at high basal levels relative to other HXTs, repression of expression by high glucose requires SNF3[1][2][3]



Community Commentary

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: CEN.PK2-1C
Genotype: EBY4000 (hxt)
Together with: HXT15, MPH2, HXT7, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11
Phenotype(s): Viable , Conditionally inviable when on glucose medium

The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. [4] [5]


DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically lower expression in sulfur limited chemostat cultures versus sulfur excess. [6] [7]


References

See Help:References on how to add references

  1. Liang H and Gaber RF (1996) A novel signal transduction pathway in Saccharomyces cerevisiae defined by Snf3-regulated expression of HXT6. Mol Biol Cell 7(12):1953-66 SGD PMID 8970157
  2. Reifenberger E, et al. (1997) Kinetic characterization of individual hexose transporters of Saccharomyces cerevisiae and their relation to the triggering mechanisms of glucose repression. Eur J Biochem 245(2):324-33 SGD PMID 9151960
  3. Ozcan S and Johnston M (1999) Function and regulation of yeast hexose transporters. Microbiol Mol Biol Rev 63(3):554-69 SGD PMID 10477308
  4. Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 SGD PMID 10618490
  5. submitted by Eckhard Boles on 2003-03-17
  6. Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  7. submitted by Viktor Boer on 2003-07-25

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