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− | === Alleles, Strains, and Phenotypes ===
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− | [[Category:Topic:Alleles, Strains, and Phenotypes]]
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− | ==== Complete Deletion ====
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− | [[Category:Topic:Alleles, Strains, and Phenotypes:Complete Deletion]]
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− | '''Allele''': reg1::URA3<br>
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4569<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | reg1 mutants are consitutively filamentous (filamentous even in the presence of glucose, unlike wild-type, which filaments on media lacking glucose). <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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− |
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− | {{ShortCenteredHR}}
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− | '''Allele''': reg1::URA3<br>
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4569<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | reg1 mutants display a constitutive invasive phenotype (they invade better than wild-type on medium containing high glucose (2%), but about the same as wild-type in limiting glucose (0.1%)). <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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− |
| |
− | {{ShortCenteredHR}}
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− | '''Allele''': reg1::URA3<br>
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4569<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | reg1 mutants are consitutively filamentous (filamentous even in the presence of glucose, unlike wild-type, which filaments on media lacking glucose). <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− | '''Allele''': reg1::URA3<br>
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4569<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | reg1 mutants display a constitutive invasive phenotype (they invade better than wild-type on medium containing high glucose (2%), but about the same as wild-type in limiting glucose (0.1%)). <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− | '''Allele''': reg1::URA3<br>
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4462<br>
| |
− | '''Together with''': SNF1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | reg1 mutants invade better than wild-type. <ref name='S000070028'>Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 {{SGDpaper|S000070028}} PMID 12024013</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− | ==== Multiple Knockout Strains ====
| |
− | [[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4469<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | Deletion of FLO11/MUC1 suppresses reg1's constitutive invasion phenotype, but not its constitutive filamentous phenotype. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4571<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4633<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4571<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4469<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | Deletion of FLO11/MUC1 suppresses reg1's constitutive invasion phenotype, but not its constitutive filamentous phenotype. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4633<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4618<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]], FLO11/MUC1 promoter substituted with S.p.Adh+ promoter [[Category:Phenotype:FLO11/MUC1 promoter substituted with S.p.Adh+ promoter]]
| |
− |
| |
− | Deletion of REG1 in cells where FLO11/MUC1 expression is clamped (by substitution of the FLO11 promoter with the S.pombe Adh+promoter) causes an increase in invasion. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− |
| |
− | '''Strain Background''': Sigma1278b<br>
| |
− | '''Genotype''': MCY4461<br>
| |
− | '''Together with''': SNF1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | Deletion of SNF1 suppresses the hyperinvasive phenotype of reg1. <ref name='S000070028'>Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 {{SGDpaper|S000070028}} PMID 12024013</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4618<br>
| |
− | '''Together with''': SIP1, GAL83, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]], FLO11/MUC1 promoter substituted with S.p.Adh+ promoter [[Category:Phenotype:FLO11/MUC1 promoter substituted with S.p.Adh+ promoter]]
| |
− |
| |
− | Deletion of REG1 in cells where FLO11/MUC1 expression is clamped (by substitution of the FLO11 promoter with the S.pombe Adh+promoter) causes an increase in invasion. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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− |
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− | {{ShortCenteredHR}}
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| __TOC__ | | __TOC__ |
| ==Community Commentary== | | ==Community Commentary== |
Share your knowledge...Edit this entry!
Systematic name |
YDR028C
|
Gene name |
REG1
|
Aliases |
HEX2, PZF240, SPP43, SRN1
|
Feature type |
ORF, Verified
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Coordinates
|
Chr IV:500876..497832
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Don't edit this box! It's automatically regenerated, and edits will be lost when the update script runs.
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Description of YDR028C: Regulatory subunit of type 1 protein phosphatase Glc7p, involved in negative regulation of glucose-repressible genes[1][2][3]
About Community Commentary. Please share your knowledge!
Alleles, Strains, and Phenotypes
Complete Deletion
Allele: reg1::URA3
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4569
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)
reg1 mutants are consitutively filamentous (filamentous even in the presence of glucose, unlike wild-type, which filaments on media lacking glucose). [4] [5]
Allele: reg1::URA3
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4569
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)
reg1 mutants display a constitutive invasive phenotype (they invade better than wild-type on medium containing high glucose (2%), but about the same as wild-type in limiting glucose (0.1%)). [4] [5]
Allele: reg1::URA3
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4569
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)
reg1 mutants are consitutively filamentous (filamentous even in the presence of glucose, unlike wild-type, which filaments on media lacking glucose). [4] [5]
Allele: reg1::URA3
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4569
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)
reg1 mutants display a constitutive invasive phenotype (they invade better than wild-type on medium containing high glucose (2%), but about the same as wild-type in limiting glucose (0.1%)). [4] [5]
Allele: reg1::URA3
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4462
Together with: SNF1
Phenotype(s): Loss of function (Null)
reg1 mutants invade better than wild-type. [6] [5]
Multiple Knockout Strains
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4469
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of FLO11/MUC1 suppresses reg1's constitutive invasion phenotype, but not its constitutive filamentous phenotype. [4] [5]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4571
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). [4] [5]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4633
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. [4] [5]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4571
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). [4] [5]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4469
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of FLO11/MUC1 suppresses reg1's constitutive invasion phenotype, but not its constitutive filamentous phenotype. [4] [5]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4633
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. [4] [5]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4618
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null), FLO11/MUC1 promoter substituted with S.p.Adh+ promoter
Deletion of REG1 in cells where FLO11/MUC1 expression is clamped (by substitution of the FLO11 promoter with the S.pombe Adh+promoter) causes an increase in invasion. [4] [5]
Strain Background: Sigma1278b
Genotype: MCY4461
Together with: SNF1
Phenotype(s): Loss of function (Null)
Deletion of SNF1 suppresses the hyperinvasive phenotype of reg1. [6] [5]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4618
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null), FLO11/MUC1 promoter substituted with S.p.Adh+ promoter
Deletion of REG1 in cells where FLO11/MUC1 expression is clamped (by substitution of the FLO11 promoter with the S.pombe Adh+promoter) causes an increase in invasion. [4] [5]
References
See Help:References on how to add references
- ↑ Frederick DL and Tatchell K (1996) The REG2 gene of Saccharomyces cerevisiae encodes a type 1 protein phosphatase-binding protein that functions with Reg1p and the Snf1 protein kinase to regulate growth. Mol Cell Biol 16(6):2922-31 SGD PMID 8649403
- ↑ Tu J and Carlson M (1995) REG1 binds to protein phosphatase type 1 and regulates glucose repression in Saccharomyces cerevisiae. EMBO J 14(23):5939-46 SGD PMID 8846786
- ↑ Niederacher D and Entian KD (1987) Isolation and characterization of the regulatory HEX2 gene necessary for glucose repression in yeast. Mol Gen Genet 206(3):505-9
SGD PMID 3035346
- ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
- ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 submitted by Valmik K. Vyas on 2003-03-27
- ↑ 6.0 6.1 Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 SGD PMID 12024013
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