Difference between revisions of "YDR342C"

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=== Alleles, Strains, and Phenotypes ===
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[[Category:Topic:Alleles, Strains, and Phenotypes]]
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==== Multiple Knockout Strains ====
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[[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
  
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'''Strain Background''': CEN.PK2-1C<br>
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'''Genotype''': EBY4000 (hxt)<br>
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'''Together with''': HXT15, MPH2, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11<br>
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'''Phenotype(s)''': Viable [[Category:Phenotype:Viable]]
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, Conditionally inviable when on glucose medium
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The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. <ref name='S000056317'>Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 {{SGDpaper|S000056317}} PMID 10618490</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
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{{ShortCenteredHR}}
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=== DNA and RNA Details ===
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[[Category:Topic:DNA and RNA Details]]
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==== Other DNA and RNA Details ====
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[[Category:Topic:DNA and RNA Details:Other DNA and RNA Details]]
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'''Other Topic''': expression [[Category:Topic:expression]]
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Specifically lower expression in sulfur limited chemostat cultures versus sulfur excess. <ref name='S000073646'>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 {{SGDpaper|S000073646}} PMID 12414795</ref> <ref name = 'CAset9153-2003-07-25'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=9153 Viktor Boer] on 2003-07-25</ref>
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{{ShortCenteredHR}}
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=== Protein Details ===
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[[Category:Topic:Protein Details]]
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==== Protein Function/Process ====
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[[Category:Topic:Protein Details:Protein Function/Process]]
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The Hxt7p glucose transporter is one of four Hxt transporters (Hxt4p, Hxt5p, Hxt7p, Gal2p) able to take up xylose <ref name='S000071422'>Hamacher T, et al. (2002) Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization. Microbiology 148(Pt 9):2783-8 {{SGDpaper|S000071422}} PMID 12213924</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
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{{ShortCenteredHR}}
 
__TOC__
 
__TOC__
 
==Community Commentary==
 
==Community Commentary==
 
{{CommentaryHelp}}
 
{{CommentaryHelp}}
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=== Alleles, Strains, and Phenotypes ===
 +
[[Category:Topic:Alleles, Strains, and Phenotypes]]
 +
==== Multiple Knockout Strains ====
 +
[[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
 +
 +
'''Strain Background''': CEN.PK2-1C<br>
 +
'''Genotype''': EBY4000 (hxt)<br>
 +
'''Together with''': HXT15, MPH2, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11<br>
 +
'''Phenotype(s)''': Viable [[Category:Phenotype:Viable]]
 +
, Conditionally inviable when on glucose medium
 +
 +
The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. <ref name='S000056317'>Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 {{SGDpaper|S000056317}} PMID 10618490</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
 +
 +
{{ShortCenteredHR}}
 +
=== DNA and RNA Details ===
 +
[[Category:Topic:DNA and RNA Details]]
 +
==== Other DNA and RNA Details ====
 +
[[Category:Topic:DNA and RNA Details:Other DNA and RNA Details]]
 +
'''Other Topic''': expression [[Category:Topic:expression]]
 +
 +
Specifically lower expression in sulfur limited chemostat cultures versus sulfur excess. <ref name='S000073646'>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 {{SGDpaper|S000073646}} PMID 12414795</ref> <ref name = 'CAset9153-2003-07-25'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=9153 Viktor Boer] on 2003-07-25</ref>
 +
 +
{{ShortCenteredHR}}
 +
=== Protein Details ===
 +
[[Category:Topic:Protein Details]]
 +
==== Protein Function/Process ====
 +
[[Category:Topic:Protein Details:Protein Function/Process]]
 +
 +
The Hxt7p glucose transporter is one of four Hxt transporters (Hxt4p, Hxt5p, Hxt7p, Gal2p) able to take up xylose <ref name='S000071422'>Hamacher T, et al. (2002) Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization. Microbiology 148(Pt 9):2783-8 {{SGDpaper|S000071422}} PMID 12213924</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
  
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{{ShortCenteredHR}}
 
==References==
 
==References==
 
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Revision as of 16:05, 23 January 2007

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Systematic name YDR342C
Gene name HXT7
Aliases
Feature type ORF, Verified
Coordinates Chr IV:1155922..1154210
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Description of YDR342C: High-affinity glucose transporter of the major facilitator superfamily, nearly identical to Hxt6p, expressed at high basal levels relative to other HXTs, expression repressed by high glucose levels[1][2]



Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: CEN.PK2-1C
Genotype: EBY4000 (hxt)
Together with: HXT15, MPH2, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11
Phenotype(s): Viable , Conditionally inviable when on glucose medium

The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. [3] [4]


DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically lower expression in sulfur limited chemostat cultures versus sulfur excess. [5] [6]


Protein Details

Protein Function/Process

The Hxt7p glucose transporter is one of four Hxt transporters (Hxt4p, Hxt5p, Hxt7p, Gal2p) able to take up xylose [7] [4]


Community Commentary

About Community Commentary. Please share your knowledge!

Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: CEN.PK2-1C
Genotype: EBY4000 (hxt)
Together with: HXT15, MPH2, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11
Phenotype(s): Viable , Conditionally inviable when on glucose medium

The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. [3] [4]


DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically lower expression in sulfur limited chemostat cultures versus sulfur excess. [5] [6]


Protein Details

Protein Function/Process

The Hxt7p glucose transporter is one of four Hxt transporters (Hxt4p, Hxt5p, Hxt7p, Gal2p) able to take up xylose [7] [4]


References

See Help:References on how to add references

  1. Reifenberger E, et al. (1997) Kinetic characterization of individual hexose transporters of Saccharomyces cerevisiae and their relation to the triggering mechanisms of glucose repression. Eur J Biochem 245(2):324-33 SGD PMID 9151960
  2. Ozcan S and Johnston M (1999) Function and regulation of yeast hexose transporters. Microbiol Mol Biol Rev 63(3):554-69 SGD PMID 10477308
  3. 3.0 3.1 Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 SGD PMID 10618490
  4. 4.0 4.1 4.2 4.3 submitted by Eckhard Boles on 2003-03-17
  5. 5.0 5.1 Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  6. 6.0 6.1 submitted by Viktor Boer on 2003-07-25
  7. 7.0 7.1 Hamacher T, et al. (2002) Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization. Microbiology 148(Pt 9):2783-8 SGD PMID 12213924

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