Difference between revisions of "YBR101C"
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'''Allele''': deltafes1<br> | '''Allele''': deltafes1<br> | ||
| − | '''Strain Background''': W303, RSY801<br> | + | '''Strain Background''': W303 (''see [[CommunityW303.html|detailed notes]] from Rodney Rothstein and Stephan Bartsch for the W303 strain used in the study''), RSY801<br> |
'''Phenotype(s)''': Temperature(Heat) Sensitive [[Category:Phenotype:Temperature(Heat) Sensitive]] | '''Phenotype(s)''': Temperature(Heat) Sensitive [[Category:Phenotype:Temperature(Heat) Sensitive]] | ||
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| − | '''Strain Background''': W303<br> | + | '''Strain Background''': W303 (''see [[CommunityW303.html|detailed notes]] from Rodney Rothstein and Stephan Bartsch for the W303 strain used in the study'') |
| + | <br> | ||
'''Mutation type(s)''': deletion, point mutation (ydj1-151) | '''Mutation type(s)''': deletion, point mutation (ydj1-151) | ||
Revision as of 06:24, 19 June 2009
Share your knowledge...Edit this entry! <protect>
| Systematic name | YBR101C |
| Gene name | FES1 |
| Aliases | |
| Feature type | ORF, Verified |
| Coordinates | Chr II:444687..443815 |
| Primary SGDID | S000000305 |
Description of YBR101C: Hsp70 (Ssa1p) nucleotide exchange factor, cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum[1]
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Contents
Community Commentary
About Community Commentary. Please share your knowledge!
Alleles, Strains, and Phenotypes
Complete Deletion
Allele: deltafes1
Strain Background: W303 (see detailed notes from Rodney Rothstein and Stephan Bartsch for the W303 strain used in the study), RSY801
Phenotype(s): Temperature(Heat) Sensitive
The deletion of the FES1 gene leads to a strong thermosensitive phenotype as well as cycloheximide sensitivity [1] [2]
Interactions
Genetic
YBR101C is a suppressor of YDJ1
Strain Background: W303 (see detailed notes from Rodney Rothstein and Stephan Bartsch for the W303 strain used in the study)
Mutation type(s): deletion, point mutation (ydj1-151)
The thermosensitivity and cycloheximide sensitivity of the single deltafes1 and ydj1-151 mutants is partly suppressed in the double mutant. [1] [2]
Physical
Physical interaction with SSA1
Purified GST-Fes1p interacts with the ADP-bound form of (His6)-Ssa1p in GST pull down assays [1] [2]
Protein Details
Protein Function/Process
Fes1p is a nucleotide exchange factor that binds to the Hsp70 chaperone Ssa1p and inhibits its ATPase activity. Fes1p does not play an obvious role in protein folding, transport or degradation, but is involved in protein translation (presumably at the initiation step) [1] [2]
Protein Localization
Fes1p is a cytosolic protein (as shown by immunofluorescence and cell fractionation assays using a GFP-Fes1p construct) [1] [2]
Techniques and Reagents
Protein Purification
Fes1p was purified as a GST fusion protein from E. coli [1] [2]
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References
See Help:References on how to add references
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Kabani M, et al. (2002) Nucleotide exchange factor for the yeast Hsp70 molecular chaperone Ssa1p. Mol Cell Biol 22(13):4677-89
SGD PMID 12052876 Cite error: Invalid
<ref>tag; name "S000072758" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072758" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072758" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072758" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072758" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072758" defined multiple times with different content - ↑ 2.0 2.1 2.2 2.3 2.4 2.5 submitted by Mehdi Kabani on 2003-03-31
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
- Pages with reference errors
- ORF
- ORF, Verified
- Topic:Alleles, Strains, and Phenotypes
- Topic:Alleles, Strains, and Phenotypes:Complete Deletion
- Phenotype:Temperature(Heat) Sensitive
- Topic:Interactions
- Topic:Interactions:Genetic
- Genetic Interaction with YDJ1
- Topic:Interactions:Physical
- Physical Interactions with SSA1
- Topic:Protein Details
- Topic:Protein Details:Protein Function/Process
- Topic:Protein Details:Protein Localization
- Topic:Techniques and Reagents
- Topic:Techniques and Reagents:Protein Purification
