Reagents

• Antibodies made to S. cerevisiae proteins

• Custom Oligos and Primers - Several companies provide custom oligo synthesis, including but not limited to, those listed here:
  • GeneWorks
  • Integrated DNA Technologies
  • Invitrogen
  • Find other companies by searching Google

• MORF collection is a moveable ORF library which enables protein expression and purification for more than 4900 S. cerevisiae genes, as described in Gelperin DM, et al. (2005).
  • Comments on MORF collection

• pAG Vectors is a collection of 288 yeast Gateway expression vectors. These "Advanced Gateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have made all of these vectors available through the non-profit plasmid repository Addgene. Clones were created by the Lindquist Lab at the Whitehead Institute. "A suite of Gateway vectors for high-throughput genetic analysis in Saccharomyces cerevisiae". Simon Alberti, Aaron D. Gitler, and Susan Lindquist." 2007. Yeast. In Press.

• The PlasmID repository distributes over 5,200 fully sequence-verified S. cerevisiae genes in Gateway entry vectors and expression vectors. The PlasmID repository is part of the DF/HCC DNA Resource Core at the Harvard Institute of Proteomics at Harvard Medical School.

• Tritech Research maintains a list of links to S. cerevisiae information and protocols, and sells reagents and equipment for S. cerevisiae research.

• VectorDB, collected sequences of cloning vectors for use in a variety of organisms

• Microarray methods for profiling the Yeast Knockout (YKO) Collection