Difference between revisions of "Reagents"
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*[http://www.addgene.org/yeast_gateway/ pAG Vectors] is a collection of 288 yeast Gateway expression vectors. These "<u>A</u>dvanced <u>G</u>ateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have made all of these vectors available through the non-profit plasmid repository [http://www.addgene.org/ Addgene]. Clones were created by the [http://web.wi.mit.edu/lindquist/pub/ Lindquist Lab] at the [http://www.wi.mit.edu/ Whitehead Institute]. "A suite of Gateway vectors for high-throughput genetic analysis in <i>Saccharomyces cerevisiae</i>". Simon Alberti, Aaron D. Gitler, and Susan Lindquist." 2007. <i>Yeast</i>. In Press. | *[http://www.addgene.org/yeast_gateway/ pAG Vectors] is a collection of 288 yeast Gateway expression vectors. These "<u>A</u>dvanced <u>G</u>ateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have made all of these vectors available through the non-profit plasmid repository [http://www.addgene.org/ Addgene]. Clones were created by the [http://web.wi.mit.edu/lindquist/pub/ Lindquist Lab] at the [http://www.wi.mit.edu/ Whitehead Institute]. "A suite of Gateway vectors for high-throughput genetic analysis in <i>Saccharomyces cerevisiae</i>". Simon Alberti, Aaron D. Gitler, and Susan Lindquist." 2007. <i>Yeast</i>. In Press. | ||
| − | *[http://plasmid.med.harvard.edu/PLASMID/ PlasmID], | + | *The [http://plasmid.med.harvard.edu/PLASMID/ PlasmID] repository distributes over 5,200 fully sequence-verified <i>S. cerevisiae</i> genes in Gateway entry vectors and expression vectors. The PlasmID repository is part of the DF/HCC DNA Resource Core at the [http://www.hip.harvard.edu Harvard Institute of Proteomics] at [http://hms.harvard.edu Harvard Medical School]. |
*[http://www.tritechresearch.com/cgi-bin/shop/yeast.html Tritech Research] maintains a list of links to <i>S. cerevisiae</i> information and protocols, and sells reagents and equipment for <i>S. cerevisiae</i> research. | *[http://www.tritechresearch.com/cgi-bin/shop/yeast.html Tritech Research] maintains a list of links to <i>S. cerevisiae</i> information and protocols, and sells reagents and equipment for <i>S. cerevisiae</i> research. | ||
*[http://seq.yeastgenome.org/vectordb/ VectorDB], collected sequences of cloning vectors for use in a variety of organisms | *[http://seq.yeastgenome.org/vectordb/ VectorDB], collected sequences of cloning vectors for use in a variety of organisms | ||
Revision as of 07:27, 23 September 2008
- Antibodies made to S. cerevisiae proteins
- Custom Oligos and Primers - Several companies provide custom oligo synthesis, including but not limited to, those listed here:
- GeneWorks
- Integrated DNA Technologies
- Invitrogen
- Find other companies by searching Google
- MORF collection is a moveable ORF library which enables protein expression and purification for more than 4900 S. cerevisiae genes, as described in Gelperin DM, et al. (2005).
- pAG Vectors is a collection of 288 yeast Gateway expression vectors. These "Advanced Gateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have made all of these vectors available through the non-profit plasmid repository Addgene. Clones were created by the Lindquist Lab at the Whitehead Institute. "A suite of Gateway vectors for high-throughput genetic analysis in Saccharomyces cerevisiae". Simon Alberti, Aaron D. Gitler, and Susan Lindquist." 2007. Yeast. In Press.
- The PlasmID repository distributes over 5,200 fully sequence-verified S. cerevisiae genes in Gateway entry vectors and expression vectors. The PlasmID repository is part of the DF/HCC DNA Resource Core at the Harvard Institute of Proteomics at Harvard Medical School.
- Tritech Research maintains a list of links to S. cerevisiae information and protocols, and sells reagents and equipment for S. cerevisiae research.
- VectorDB, collected sequences of cloning vectors for use in a variety of organisms
