YPL068C - Revision history

Teagueb: Fixed some formatting

2019-05-31T19:49:41Z

Fixed some formatting

Teagueb: /* Community Commentary */

2019-05-10T02:57:22Z

‎Community Commentary

Teagueb: /* Cold Shock Sensitivity */

2019-05-10T00:59:36Z

‎Cold Shock Sensitivity

Teagueb: /* Cold Shock Sensitivity */

2019-05-10T00:37:03Z

‎Cold Shock Sensitivity

Teagueb: /* Community Commentary */

2019-05-10T00:35:27Z

‎Community Commentary

Teagueb: /* Community Commentary */

2019-05-10T00:35:06Z

‎Community Commentary

Teagueb at 21:23, 9 May 2019

2019-05-09T21:23:10Z

Teagueb: /* Heat Shock */

2019-05-07T15:47:27Z

‎Heat Shock

Teagueb: /* Caffeine Sensitivity */

2019-05-06T21:28:35Z

‎Caffeine Sensitivity

Teagueb at 21:26, 6 May 2019

2019-05-06T21:26:54Z

@@ Line 28: / Line 28: @@
 {{CommentaryHelp}}
 ===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]===
@@ Line 36: / Line 43: @@
 YPL068C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
 ===[[UW-Stout/G-418 | G-418 Stress]]===
@@ Line 66: / Line 64: @@
 ===Stressing with Hydroxyurea===
 [[UW-Stout/Hydroxyurea|Hydroxyurea Protocol]]
-<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
+[[file:HU_YPL068C.png]]
-<!--
-Specifically higher expression in carbon limited chemostat cultures versus carbon excess.
-<ref>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur.
+The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YPL068C showed about a 30 minute difference in doubling time compared to the wild type strain, indicating that this gene most likely plays some role in DNA replication.
-J Biol Chem 278(5):3265-74</ref>
 ===Fermentation===
-Fermentation protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/Fermentation]
+Fermentation protocol link [[https://wiki.yeastgenome.org/index.php/UW-Stout/Fermentation]]
-- There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.
 [[File:Fer YPL068C.PNG]]
-<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
 ===Caffeine Sensitivity===
-[[File:YPL068C.jpg|YPL068C VS BY4735]] Caffeine PRotocol [[UW-Stout/Caffeine], the genes reacted the same at the beginning, varied in the middle then came back together at the end.
+[[File:YPL068C.jpg|YPL068C VS BY4735]]
 ===Stressing with pH===
 pH stress protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/pH]
@@ Line 105: / Line 100: @@
 ===Cold Shock Sensitivity===
 [[File:YPL068CBB.png]]
 Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock Cold Shock]
+<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
-YPL068C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YPL068C.
+<!--
 ==References==
 <!-- REFERENCES ARE AUTOMATICALLY GENERATED.  PLEASE DON'T EDIT THIS SECTION-->
 {{RefHelp}}

@@ Line 106: / Line 106: @@
 ===Cold Shock Sensitivity===
 [[File:YPL068CBB.png]]
-Cold Shock Protocol [[https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock]]
@@ Line 114: / Line 114: @@
+YPL068C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YPL068C.
 ==References==
 <!-- REFERENCES ARE AUTOMATICALLY GENERATED.  PLEASE DON'T EDIT THIS SECTION-->
 {{RefHelp}}

← Older revision		Revision as of 00:59, 10 May 2019
Line 107:		Line 107:
	[[File:YPL068CBB.png]]		[[File:YPL068CBB.png]]
	Cold Shock Protocol [[https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock]]		Cold Shock Protocol [[https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock]]
		+
		+
		+
		+	Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock Cold Shock]
		+

@@ Line 107: / Line 107: @@
 [[File:YPL068CBB.png]]
 Cold Shock Protocol [[https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock]]
 ==References==
 <!-- REFERENCES ARE AUTOMATICALLY GENERATED.  PLEASE DON'T EDIT THIS SECTION-->
 {{RefHelp}}

@@ Line 105: / Line 105: @@
 ===Cold Shock Sensitivity===
-[[File:YPL068C.png]]
+[[File:YPL068CBB.png]]
 Cold Shock Protocol [[https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock]]

@@ Line 54: / Line 54: @@
 ===[[UW-Stout/Heat Shock|Heat Shock]]===
 [[File:HeatShockforYP.png]]
 ===Methanol Sensitivity===