YOR387C - Revision history

Teagueb: /* Doubling Time at 0.25 concentration */

2021-05-05T14:49:04Z

‎Doubling Time at 0.25 concentration

Teagueb: /* Caffeine Group 2 */

2021-05-05T12:24:48Z

‎Caffeine Group 2

Teagueb: /* Doubling Time at 0.25 concentration */

2021-05-05T12:23:18Z

‎Doubling Time at 0.25 concentration

Teagueb: /* Caffeine Group 2 */

2021-05-05T06:48:21Z

‎Caffeine Group 2

Teagueb: /* Caffeine Group 2 */

2021-05-05T06:47:27Z

‎Caffeine Group 2

Teagueb: /* Hydroxyurea */

2021-05-03T17:20:03Z

‎Hydroxyurea

Teagueb: /* Ethanol Sensitivity */

2021-05-02T03:01:23Z

‎Ethanol Sensitivity

Teagueb: /* Ethanol Sensitivity */

2021-05-01T06:52:26Z

‎Ethanol Sensitivity

Teagueb: /* Hydroxyurea */

2021-05-01T03:43:14Z

‎Hydroxyurea

Teagueb: /* Ethanol Sensitivity */

2021-05-01T03:41:27Z

‎Ethanol Sensitivity

@@ Line 132: / Line 132: @@
 [[File:YOR387C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YOR387C Td = (199-1) x ln(2)/ln(0.25/0.1) = 150 minutes  These doubling times show that the 0.25 mM concentration in YOR387C slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
+Wild Type w/ caffeine Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; Wild Type no caffeine Td= (113-13) x ln(2)/ln(.5/.1)= 34 minutes; YOR387C w/ caffeine Td = (199-1) x ln(2)/ln(0.25/0.1) = 150 minutes ; YOR387C no caffeine Td= (121-25) x ln(2)/ln(.6/.1)= 37 minutes; These doubling times show that the 0.25 mM concentration in YOR387C slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
 ==[[UW-Stout/Heat Shock SP21|Heat Shock]]==

@@ Line 132: / Line 132: @@
 [[File:YOR387C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YOR387C Td = (199-1) x ln(2)/ln(0.25/0.1) = 150 minutes The caffeine was able to slow down the growth rate of YOR387C. These doubling times show that the 0.25 mM concentration in YBR225W slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
+Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YOR387C Td = (199-1) x ln(2)/ln(0.25/0.1) = 150 minutes  These doubling times show that the 0.25 mM concentration in YOR387C slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
 ==[[UW-Stout/Heat Shock SP21|Heat Shock]]==

@@ Line 132: / Line 132: @@
 [[File:YOR387C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YOR387C Td = (199-1) x ln(2)/ln(0.25/0.1) = 150 minutes
+Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YOR387C Td = (199-1) x ln(2)/ln(0.25/0.1) = 150 minutes The caffeine was able to slow down the growth rate of YOR387C. These doubling times show that the 0.25 mM concentration in YBR225W slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
 ==[[UW-Stout/Heat Shock SP21|Heat Shock]]==

@@ Line 130: / Line 130: @@
 ----
 ===[[UW-Stout/Caffeine 2 SP21|Caffeine Group 2]]===
-[[File:Example.jpg]]
+[[File:YOR387C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
 Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YOR387C Td = (199-1) x ln(2)/ln(0.25/0.1) = 150 minutes

@@ Line 129: / Line 129: @@
 From this data we can determine that there was a moderate growth inhibition on this strain of yeast. The modified yeast had a doubling rate that was eight minutes longer than the wild type on average, and when compared to the effect on other yeast strains this is a small difference, but not insignificant.
 ----
-===[[UW-Stout/Caffeine_SP21|Caffeine Group 2]]===
+===[[UW-Stout/Caffeine 2 SP21|Caffeine Group 2]]===
-[[File:YOR387C-caffeine.png|700px]]
+[[File:Example.jpg]]
 ====Doubling Time at 0.25 concentration====
 Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YOR387C Td = (199-1) x ln(2)/ln(0.25/0.1) = 150 minutes

← Older revision		Revision as of 17:20, 3 May 2021
Line 151:		Line 151:

	The YOR387C strain was to have an average doubling time of 183 minutes, which was slightly higher than the doubling time of the BY4735 strain, with 182 minutes. The doubling time of the BY4735 strain when exposed to hydroxyurea was 289 and a half minutes and the doubling time of the YOR387C strain 269 minutes. Hydroxyurea seems to impact these strains slightly in terms of being more sensitive to this stress, but overall not a drastic impact.		The YOR387C strain was to have an average doubling time of 183 minutes, which was slightly higher than the doubling time of the BY4735 strain, with 182 minutes. The doubling time of the BY4735 strain when exposed to hydroxyurea was 289 and a half minutes and the doubling time of the YOR387C strain 269 minutes. Hydroxyurea seems to impact these strains slightly in terms of being more sensitive to this stress, but overall not a drastic impact.
		+	[https://wiki.yeastgenome.org/index.php/UW-Stout/Hydroxyurea_SP21]

	==References==		==References==

← Older revision		Revision as of 03:01, 2 May 2021
Line 144:		Line 144:

	The YOR387C yeast strain was determined to have an average doubling time of 216 minutes, which is only slightly higher to the doubling time of the BY4735 strain, at 182 minutes. The doubling time of the BY4735 strain when exposed to 9ethanol is 218 minutes, and the doubling time of the YOR387C strain is 290 minutes when exposed to 9% concentration of ethanol. Ethanol does not seem to impact either of these strains too drastically in terms of one being more or less sensitive to this stress.		The YOR387C yeast strain was determined to have an average doubling time of 216 minutes, which is only slightly higher to the doubling time of the BY4735 strain, at 182 minutes. The doubling time of the BY4735 strain when exposed to 9ethanol is 218 minutes, and the doubling time of the YOR387C strain is 290 minutes when exposed to 9% concentration of ethanol. Ethanol does not seem to impact either of these strains too drastically in terms of one being more or less sensitive to this stress.
		+
		+	[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]

	== Hydroxyurea ==		== Hydroxyurea ==

@@ Line 143: / Line 143: @@
 [[File:YOR387C Ethanol.png]]
-The YOR387C yeast strain was determined to have an average doubling time of 216 minutes, which is only slightly higher to the doubling time of the BY4735 strain, at 182 minutes. The doubling time of the BY4735 strain when exposed to ethanol is 218 minutes, and the doubling time of the YOR387C strain is 290 minutes.  Ethanol does not seem to impact either of these strains too drastically in terms of one being more or less sensitive to this stress.
+The YOR387C yeast strain was determined to have an average doubling time of 216 minutes, which is only slightly higher to the doubling time of the BY4735 strain, at 182 minutes. The doubling time of the BY4735 strain when exposed to 9ethanol is 218 minutes, and the doubling time of the YOR387C strain is 290 minutes when exposed to 9% concentration of ethanol.  Ethanol does not seem to impact either of these strains too drastically in terms of one being more or less sensitive to this stress.
 == Hydroxyurea ==

@@ Line 147: / Line 147: @@
 == Hydroxyurea ==
-[[File:growthratestress.png]]
+[[File:growthratestress.png|center|thumb|1000px|]]
 The YOR387C strain was to have an average doubling time of 183 minutes, which was slightly higher than the doubling time of the BY4735 strain, with 182 minutes. The doubling time of the BY4735 strain when exposed to hydroxyurea was 289 and a half minutes and the doubling time of the YOR387C strain 269 minutes. Hydroxyurea seems to impact these strains slightly in terms of being more sensitive to this stress, but overall not a drastic impact.

← Older revision		Revision as of 03:41, 1 May 2021
Line 144:		Line 144:

	The YOR387C yeast strain was determined to have an average doubling time of 216 minutes, which is only slightly higher to the doubling time of the BY4735 strain, at 182 minutes. The doubling time of the BY4735 strain when exposed to ethanol is 218 minutes, and the doubling time of the YOR387C strain is 290 minutes. Ethanol does not seem to impact either of these strains too drastically in terms of one being more or less sensitive to this stress.		The YOR387C yeast strain was determined to have an average doubling time of 216 minutes, which is only slightly higher to the doubling time of the BY4735 strain, at 182 minutes. The doubling time of the BY4735 strain when exposed to ethanol is 218 minutes, and the doubling time of the YOR387C strain is 290 minutes. Ethanol does not seem to impact either of these strains too drastically in terms of one being more or less sensitive to this stress.
		+
		+
		+	== Hydroxyurea ==
		+	[[File:growthratestress.png]]
		+
		+	The YOR387C strain was to have an average doubling time of 183 minutes, which was slightly higher than the doubling time of the BY4735 strain, with 182 minutes. The doubling time of the BY4735 strain when exposed to hydroxyurea was 289 and a half minutes and the doubling time of the YOR387C strain 269 minutes. Hydroxyurea seems to impact these strains slightly in terms of being more sensitive to this stress, but overall not a drastic impact.

	==References==		==References==