YOR111W - Revision history

Teagueb: /* Community Commentary */

2022-05-12T03:33:30Z

‎Community Commentary

Teagueb: /* Results */

2022-05-12T03:28:30Z

‎Results

Teagueb: /* Interpretation */

2022-05-12T03:04:18Z

‎Interpretation

Teagueb: /* Interpretation */

2022-05-12T03:03:18Z

‎Interpretation

Teagueb: /* Interpretation */

2022-05-12T03:00:37Z

‎Interpretation

Teagueb: /* Community Commentary */

2022-05-12T02:32:12Z

‎Community Commentary

Teagueb: /* Results */

2022-05-09T05:06:26Z

‎Results

Teagueb: /* Community Commentary */

2022-05-09T03:21:33Z

‎Community Commentary

Teagueb at 17:36, 8 May 2022

2022-05-08T17:36:22Z

Teagueb at 00:48, 7 May 2022

2022-05-07T00:48:31Z

← Older revision		Revision as of 03:33, 12 May 2022
Line 168:		Line 168:

	Based on the growth curves, we can see that the YOR111W control is growing at a faster rate than both the wild type control and the stressed wild type. However, the YOR111W being treated with Nystatin is growing at a slower rate than both wild type control and stressed wild type. This indicates that the removal of the YOR111W gene lessens the cells resistance to Nystatin.		Based on the growth curves, we can see that the YOR111W control is growing at a faster rate than both the wild type control and the stressed wild type. However, the YOR111W being treated with Nystatin is growing at a slower rate than both wild type control and stressed wild type. This indicates that the removal of the YOR111W gene lessens the cells resistance to Nystatin.
		+
		+
		+	===[[UW-Stout/Formamide SP22]]===

@@ Line 172: / Line 172: @@
 ===Results===
-[[File:wildyeast.jpg|400px|]] [[File:trial222.jpg|400px|]] [[File:doublingtimestable.jpg|400px|]]
+[[File:wildyeast.jpg|400px|]] [[File:yeast.jpg|400px|]] [[File:doublingtimestable.jpg|400px|]]
@@ Line 178: / Line 178: @@
 *Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
 *Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)
 ===Interpretation===

@@ Line 181: / Line 181: @@
 ===Interpretation===
-The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YOR11W strain. The YOR11W strain had doubling times significantly faster than the wild yeast cell, with a doubling time of 637.92 min. It can be concluded that the transformed yeast cell, YOR11W, had a unique response to the formamide stress test used in the experiment.
+The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YOR111W strain. The YOR111W strain had doubling times significantly faster than the wild yeast cell, with a doubling time of 637.92 min. It can be concluded that the transformed yeast cell, YOR111W, had a unique response to the formamide stress test used in the experiment.
 ==References==

@@ Line 181: / Line 181: @@
 ===Interpretation===
-The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. Where some of the transformed yeast cells were heavily effected by the formamide, the YOR11W strain had doubling times just slightly slower than the wild yeast cell, with a doubling time of 844.12 min. It can be concluded that the transformed yeast cell, YOR11W, did not have a unique response to the formamide stress test used in the experiment.
+The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YOR11W strain. The YOR11W strain had doubling times significantly faster than the wild yeast cell, with a doubling time of 637.92 min. It can be concluded that the transformed yeast cell, YOR11W, had a unique response to the formamide stress test used in the experiment.
 ==References==

← Older revision		Revision as of 03:00, 12 May 2022
Line 181:		Line 181:

	===Interpretation===		===Interpretation===
		+	The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. Where some of the transformed yeast cells were heavily effected by the formamide, the YOR11W strain had doubling times just slightly slower than the wild yeast cell, with a doubling time of 844.12 min. It can be concluded that the transformed yeast cell, YOR11W, did not have a unique response to the formamide stress test used in the experiment.

	==References==		==References==

← Older revision		Revision as of 02:32, 12 May 2022
Line 168:		Line 168:

	Based on the growth curves, we can see that the YOR111W control is growing at a faster rate than both the wild type control and the stressed wild type. However, the YOR111W being treated with Nystatin is growing at a slower rate than both wild type control and stressed wild type. This indicates that the removal of the YOR111W gene lessens the cells resistance to Nystatin.		Based on the growth curves, we can see that the YOR111W control is growing at a faster rate than both the wild type control and the stressed wild type. However, the YOR111W being treated with Nystatin is growing at a slower rate than both wild type control and stressed wild type. This indicates that the removal of the YOR111W gene lessens the cells resistance to Nystatin.
		+
		+
		+
		+	===Results===
		+	[[File:wildyeast.jpg\|400px\|]] [[File:trial222.jpg\|400px\|]] [[File:doublingtimestable.jpg\|400px\|]]
		+
		+
		+	*3% formamide line graph: x-axis= growing time (min); y-axis= optical density 600
		+	*Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
		+	*Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)
		+
		+
		+	===Interpretation===

	==References==		==References==

@@ Line 166: / Line 166: @@
 [[File:YOR-T1.jpg]]
 [[File:YOR-T2.jpg]]
 ==References==

@@ Line 160: / Line 160: @@
 ====Interpretation====
 The growth curve suggests that YOR111W may have some level of decreased sensitivity to oxidative stress. Both the knockout and control exhibited more growth than wild type cells in the same scenarios. While the difference in range for the cells treated with hydrogen peroxide is not large, it is at least notable as the knockout stayed above wild type consistently.
 ==References==

← Older revision		Revision as of 17:36, 8 May 2022
Line 179:		Line 179:

	-We can then conclude that the knocked-out gene in YOR111W doesn't have a significant impact on the growth of the yeast cell.		-We can then conclude that the knocked-out gene in YOR111W doesn't have a significant impact on the growth of the yeast cell.
		+
		+
		+	'''[[UW-Stout/pH Acid SP22]]'''
		+
		+	[[File:Strain3.jpg\|350px\|]]
		+	Knocked out gene seems to have some effect on cell growth in pH 7 environment based on the flattening of the growth curve. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve. Please see protocol for specific quantitative doubling time results.
		+
		+
		+	[[File:Wild (1).jpg\|350px\|]]
		+	The wild-type strain was used as a control in this experiment; no genes were knocked out, it just represents how the experiment ran on the knocked-out gene strains affects a "typical" cell growth curve. Inclusion of this data on each knock-out strain growth curve graph made for difficult interpretation, so it has been separated. Please refer to this graph as a control when viewing the knock-out strain growth curve graph. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve.

← Older revision		Revision as of 00:48, 7 May 2022
Line 165:		Line 165:
	{{RefHelp}}		{{RefHelp}}
	</protect>		</protect>
		+
		+	'''[[UW-Stout/Nonionic Detergent SP22]]'''
		+
		+	As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 0.001% concentrated NLS. (Nonionic detergent)
		+
		+	[[File:YOR111W.png\|500px]]
		+
		+	'''Interpretations:'''
		+
		+	-According to the graph above, we can see that the KO yeast cell YOR111W follow a smaller sensitivity to the stress than the Wild type strain.
		+
		+	-The KO with NLS peaks a lot later than the KO w/o NLS
		+
		+	-We can then conclude that the knocked-out gene in YOR111W doesn't have a significant impact on the growth of the yeast cell.