YNL018C - Revision history

Teagueb: /* Caffeine Group 2 */

2021-05-05T14:53:49Z

‎Caffeine Group 2

Teagueb: /* Caffeine Group 2 */

2021-05-05T12:22:00Z

‎Caffeine Group 2

Teagueb: /* Caffeine Group 2 */

2021-05-05T06:46:46Z

‎Caffeine Group 2

Teagueb: /* Caffeine Group 2 */

2021-05-05T06:43:38Z

‎Caffeine Group 2

Teagueb: /* Hydroxyurea */

2021-05-03T17:19:30Z

‎Hydroxyurea

Teagueb: /* Ethanol Sensitivity */

2021-05-02T19:55:05Z

‎Ethanol Sensitivity

Teagueb: /* Ethanol Sensitivity */

2021-05-02T03:01:01Z

‎Ethanol Sensitivity

Teagueb: /* Ethanol Sensitivity */

2021-05-01T05:39:05Z

‎Ethanol Sensitivity

Teagueb: /* Community Commentary */

2021-05-01T05:38:05Z

‎Community Commentary

Teagueb: /* Community Commentary */

2021-04-29T18:38:39Z

‎Community Commentary

@@ Line 136: / Line 136: @@
 [[File:YNL018C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YNL018C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes The caffeine was able to slow down the growth rate of YNL018C without inducing apoptosis. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
+Wild Type caffeine Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; Wild Type no caffeine Td= (113-33) x ln(2)/ln(.5/.1); YNL018C no caffeine Td= (145-25) x ln(2)/ln(.7/.1); YNL018C caffeine Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes The caffeine was able to slow down the growth rate of YNL018C without inducing apoptosis. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
 ===[[UW-Stout/Ethanol Sensitivity| Ethanol Sensitivity]]===

@@ Line 136: / Line 136: @@
 [[File:YNL018C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YNL018C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes
+Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YNL018C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes The caffeine was able to slow down the growth rate of YNL018C without inducing apoptosis. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
 ===[[UW-Stout/Ethanol Sensitivity| Ethanol Sensitivity]]===

@@ Line 134: / Line 134: @@
 ----
 ===[[UW-Stout/Caffeine 2 SP21|Caffeine Group 2]]===
-[[File:Example.jpg]]
+[[File:YNL018C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
 Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YNL018C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes

@@ Line 133: / Line 133: @@
 The top plate is the non-heat shocked sample, the middle and bottom are heat shocked. The results from this experiment showed that the YNLo19C strain of yeast is not heat sensitive. All three of the samples have similar growth and are therefore not heat sensitive.
 ----
-===[[UW-Stout/Caffeine_SP21|Caffeine Group 2]]===
+===[[UW-Stout/Caffeine 2 SP21|Caffeine Group 2]]===
-[[File:YNL018C-caffeine.png|700px]]
+[[File:Example.jpg]]
 ====Doubling Time at 0.25 concentration====
 Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YNL018C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes

← Older revision		Revision as of 17:19, 3 May 2021
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	The YNL018C yeast strain had an average doubling time of 187, while the average doubling time of the BY4735 strain was 182 minutes. After stressing the YNL018C strain with hydroxyurea, the average doubling time was 226 minutes, with the average doubling time of the BY4735 strain was 278 minutes. Therefore, the strain is less sensitive to hydroxyurea as the results from the growth curve show.		The YNL018C yeast strain had an average doubling time of 187, while the average doubling time of the BY4735 strain was 182 minutes. After stressing the YNL018C strain with hydroxyurea, the average doubling time was 226 minutes, with the average doubling time of the BY4735 strain was 278 minutes. Therefore, the strain is less sensitive to hydroxyurea as the results from the growth curve show.
		+	[https://wiki.yeastgenome.org/index.php/UW-Stout/Hydroxyurea_SP21]

	==References==		==References==

← Older revision		Revision as of 19:55, 2 May 2021
Line 144:		Line 144:

	[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]		[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]
		+
		+
		+
		+	== Hydroxyurea ==
		+	[[File:YNL018C Hydroxyurea.png\|center\|1000px\|]]
		+
		+	The YNL018C yeast strain had an average doubling time of 187, while the average doubling time of the BY4735 strain was 182 minutes. After stressing the YNL018C strain with hydroxyurea, the average doubling time was 226 minutes, with the average doubling time of the BY4735 strain was 278 minutes. Therefore, the strain is less sensitive to hydroxyurea as the results from the growth curve show.

	==References==		==References==

← Older revision		Revision as of 03:01, 2 May 2021
Line 142:		Line 142:

	The YNL018C yeast strain was determined to have an average doubling time of 220 minutes while the BY4735 strain, has a doubling time of 182 minutes. After stressing the strain out with 9% concentration of ethanol, the doubling time of the BY4735 strain when exposed to ethanol is 218 minutes, and the doubling time of the YKL121W strain is 213 minutes. Therefore, the strain is sensitive to ethanol as the results from the growth curve end in cell death.		The YNL018C yeast strain was determined to have an average doubling time of 220 minutes while the BY4735 strain, has a doubling time of 182 minutes. After stressing the strain out with 9% concentration of ethanol, the doubling time of the BY4735 strain when exposed to ethanol is 218 minutes, and the doubling time of the YKL121W strain is 213 minutes. Therefore, the strain is sensitive to ethanol as the results from the growth curve end in cell death.
		+
		+	[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]

	==References==		==References==

@@ Line 139: / Line 139: @@
 ===[[UW-Stout/Ethanol Sensitivity| Ethanol Sensitivity]]===
-[[File:YNL018C Ethanol.jpg]]
+[[File:YNL018C Ethanol.png]]
 The YNL018C yeast strain was determined to have an average doubling time of 220 minutes while the BY4735 strain, has a doubling time of 182 minutes. After stressing the strain out with 9% concentration of ethanol, the doubling time of the BY4735 strain when exposed to ethanol is 218 minutes, and the doubling time of the YKL121W strain is 213 minutes. Therefore, the strain is sensitive to ethanol as the results from the growth curve end in cell death.

← Older revision		Revision as of 18:38, 29 April 2021
Line 50:		Line 50:

	The YNL018C yeast gene was determined to have an average doubling time of 151 minutes under the stress of the drug PMSF. This is is nearly 3 times as fast as the BY4735, which had a doubling time of 382 minutes. This was determined to be likely due to the modified yeast strain having a stronger resistance to the drug, making it able to grow faster and with less of an effect. Without the PMSF, the corresponding doubling times were 204 minutes for the YNL018C strain, and 442 minutes for the BY4735 strain.		The YNL018C yeast gene was determined to have an average doubling time of 151 minutes under the stress of the drug PMSF. This is is nearly 3 times as fast as the BY4735, which had a doubling time of 382 minutes. This was determined to be likely due to the modified yeast strain having a stronger resistance to the drug, making it able to grow faster and with less of an effect. Without the PMSF, the corresponding doubling times were 204 minutes for the YNL018C strain, and 442 minutes for the BY4735 strain.
		+
		+	===[[UW-Stout/Sodium Hydroxide SP21\|Sodium Hydroxide]]===
		+	[[File:YNL vs WT.jpg]]
		+
		+	The YNL018C yeast gene was determined to have an average doubling time of 312 minutes under the stress of the base NaOH. This is is nearly twice as fast as the BY4735, which had a doubling time of 545 minutes. This was determined to be likely due to the modified yeast strain having a stronger resistance to NaOH, making it able to grow faster and with less of an effect. With just water, the corresponding doubling times were 785 minutes for the YNL018C strain, and 809 minutes for the BY4735 strain.

	<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->		<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->