YMR144W - Revision history

Teagueb: /* Caffeine */

2023-05-06T19:05:48Z

‎Caffeine

Teagueb: /* Caffeine */

2023-05-06T19:03:53Z

‎Caffeine

Teagueb at 04:11, 6 May 2023

2023-05-06T04:11:32Z

Teagueb at 22:08, 5 May 2023

2023-05-05T22:08:57Z

Teagueb at 22:00, 5 May 2023

2023-05-05T22:00:38Z

Teagueb at 22:00, 5 May 2023

2023-05-05T22:00:06Z

Teagueb at 21:59, 5 May 2023

2023-05-05T21:59:00Z

Teagueb at 21:48, 5 May 2023

2023-05-05T21:48:06Z

Teagueb at 21:45, 5 May 2023

2023-05-05T21:45:25Z

Teagueb: /* Electrical Shock */

2023-05-04T01:19:47Z

‎Electrical Shock

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 '''Analysis'''
-The average doubling time for the YMR144W gene in standard conditions was 194.2 minutes. Under the stress of the caffeine stress the average doubling time was 84 minutes. Under the stress of caffeine there was more growth that happened. This is interesting to think about because usually under some sort of stress there would be less growth. We are also able to see that there is staggered growth among the caffeine stress and that could be a cause of such high amounts of caffeine concentration. We also see that the lesser the concentration of caffeine the slower it grew as for the 6.8mL of caffeine concentration. We see that with more caffeine the knockout strain was still able to grow quite rapidly but with less caffeine stress it grew more slowly.
+The average doubling time for the YMR144W gene in standard conditions was 194.2 minutes. Under the stress of the caffeine stress the average doubling time was 84 minutes. Under the stress of caffeine there was more growth that happened. This is interesting to think about because usually under some sort of stress there would be less growth. We are also able to see that there is staggered growth among the caffeine stress and that could be a cause of such high amounts of caffeine concentration. We also see that the lesser the concentration of caffeine the slower it grew as for the 6.8mL of caffeine concentration. We see that with more caffeine the knockout strain was still able to grow quite rapidly but with less caffeine stress it grew more slowly. We also see the spiked regions in our graphs indicating that there was a lot of growth during that time frame.
 ==Isopropanol==

← Older revision		Revision as of 19:03, 6 May 2023
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	'''Analysis'''		'''Analysis'''
		+	The average doubling time for the YMR144W gene in standard conditions was 194.2 minutes. Under the stress of the caffeine stress the average doubling time was 84 minutes. Under the stress of caffeine there was more growth that happened. This is interesting to think about because usually under some sort of stress there would be less growth. We are also able to see that there is staggered growth among the caffeine stress and that could be a cause of such high amounts of caffeine concentration. We also see that the lesser the concentration of caffeine the slower it grew as for the 6.8mL of caffeine concentration. We see that with more caffeine the knockout strain was still able to grow quite rapidly but with less caffeine stress it grew more slowly.

	==Isopropanol==		==Isopropanol==

← Older revision		Revision as of 04:11, 6 May 2023
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	[[File:chart(this one).jpg]]		[[File:chart(this one).jpg]]

		+
		+	===analysis===
		+
		+	the results were pretty consistent as there were 4 similar looking results as can be seen from the graph. during the first trial, the growth was much more than expected which through off the final average however test 2-4 had similar looking results. in trial 2-4 the levels of growth matched pretty closely, YMR144W always had more growth than knockout strain 2 and 3, and was close to wild type.

← Older revision		Revision as of 22:08, 5 May 2023
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	[https://wiki.yeastgenome.org/index.php/UW-Stout/G418_SP23]		[https://wiki.yeastgenome.org/index.php/UW-Stout/G418_SP23]

−	[[File:chart.~~png\|200px\|thumb\|left\|alt text~~]]	+	[[File:chart(this one).jpg]]
		+

← Older revision		Revision as of 22:00, 5 May 2023
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	[[File:chart.png\|200px\|thumb\|left\|alt text]]		[[File:chart.png\|200px\|thumb\|left\|alt text]]
		+

	==Glucose Sensitivity==		==Glucose Sensitivity==

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 [https://wiki.yeastgenome.org/index.php/UW-Stout/G418_SP23]
-[[File:chart.png]]
+[[File:chart.png|200px|thumb|left|alt text]]
 ==Glucose Sensitivity==

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 ===Analysis===
 These results were not consistent as there are 3 very different results. The first trial was way off what it was supposed to be and therefore could have been contaminated or been an error in the pipetting process. The second trial was the most successful and gave the results that were expected. The third trial was good except for the region from about 8:00-11:20 where it spiked up and down. The last 2 had more growth than the control which could mean that the cell without the gene that was knocked out is able to withstand more alcohol.
 ==Glucose Sensitivity==

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 '''Data'''
 [[Image:BT11.jpg]]
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 '''Analysis'''
-These two pictures are showing how the knockout strains react with either no electrical shock or an electrical shock of 2.5 kV. These were the same experiments but on different days to make sure things looked like each other. With this first knockout strains we see that these acted just like our wild type cells. They show a good number of cells on our negative control while when we shocked them at 2.5kV some died but not all of them. With out second trial we noticed that we had contamination in all of our plates. While our cells did grow so did other things.
+These two pictures show how the knockout strain reacts with either no electrical shock or an electrical shock of 2.5 kV. These were the same experiments but on different days to make sure things looked like each other. With this first knockout strain, we see that these acted just like our wild type cells. They show a good number of cells on our negative control, but when we shocked them at 2.5kV, many died. With our second trial we noticed that we had contamination in all of our plates. While our cells did grow so did other things.
 ==References==