YLR426W - Revision history

Teagueb: /* Caffeine Group 2 */

2021-05-05T15:01:33Z

‎Caffeine Group 2

2021-05-05T12:24:02Z

‎Caffeine Group 2

2021-05-05T12:20:11Z

‎Doubling Time at 0.25 concentration

2021-05-05T06:45:53Z

‎Caffeine Group 2

2021-05-05T06:43:05Z

‎Caffeine Group 2

2021-05-05T06:41:55Z

‎Caffeine Group 2

2021-05-05T06:36:25Z

‎Caffeine Group 2

2021-05-05T06:35:58Z

‎Caffeine Group 2

2021-05-03T16:54:41Z

‎Ethanol Sensitivity

2021-05-02T03:24:27Z

@@ Line 135: / Line 135: @@
 [[File:YLR426W graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YLR426W Td = (217-1) x ln(2)/ln(0.1/0.09) = 1421 minutes These doubling times show that the 0.25 mM concentration in YLR426W slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
+Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; Wild Type no caffeine Td= (113-33) x ln(2)/ln(.5/.1)=34 minutes; YLR426W no caffeine Td= (193-73) x ln(2)/ln(.2/.1)= 120 minutes; YLR426W Td = (217-1) x ln(2)/ln(0.1/0.09) = 1421 minutes These doubling times show that the 0.25 mM concentration in YLR426W slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
 ===[[UW-Stout/Ethanol Sensitivity| Ethanol Sensitivity]]===

@@ Line 135: / Line 135: @@
 [[File:YLR426W graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YLR426W Td = (217-1) x ln(2)/ln(0.1/0.09) = 1421 minutes The caffeine slowed down the growth rate of YLR426W. The caffeine most likely effected the function of the p53 protein, and induced apoptosis.
+Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YLR426W Td = (217-1) x ln(2)/ln(0.1/0.09) = 1421 minutes These doubling times show that the 0.25 mM concentration in YLR426W slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
 ===[[UW-Stout/Ethanol Sensitivity| Ethanol Sensitivity]]===

@@ Line 135: / Line 135: @@
 [[File:YLR426W graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YLR426W Td = (217-1) x ln(2)/ln(0.1/0.09) = 1421 minutes
+Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YLR426W Td = (217-1) x ln(2)/ln(0.1/0.09) = 1421 minutes The caffeine slowed down the growth rate of YLR426W. The caffeine most likely effected the function of the p53 protein, and induced apoptosis.
 ===[[UW-Stout/Ethanol Sensitivity| Ethanol Sensitivity]]===

@@ Line 133: / Line 133: @@
 ----
 ===[[UW-Stout/Caffeine 2 SP21|Caffeine Group 2]]===
-[[File:YLR426W graph.png]]
+[[File:YLR426W graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
 Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YLR426W Td = (217-1) x ln(2)/ln(0.1/0.09) = 1421 minutes

@@ Line 133: / Line 133: @@
 ----
 ===[[UW-Stout/Caffeine 2 SP21|Caffeine Group 2]]===
-[[File:Example.jpg]]
+[[File:YLR426W graph.png]]
 ====Doubling Time at 0.25 concentration====
 Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YLR426W Td = (217-1) x ln(2)/ln(0.1/0.09) = 1421 minutes

@@ Line 132: / Line 132: @@
 The YLR426W yeast strain was effected the most by heat shocking than any other gene tested in this protocol/experiment. The controlled plate for this gene grew a very thick blanket of DNA colonies (comparable to the YBR225W strain). As seen in the picture, the controlled plate (labeled "X") was covered with cells, while the two trial plates had very little growth, if anything at all. This specific yeast strain was very sensitive to any sort of heat. Most other genes had at least a little growth to a decent amount, but this one failed to handle the heat shock.
 ----
-===[[UW-Stout/Caffeine SP21|Caffeine Group 2]]===
+===[[UW-Stout/Caffeine_SP21|Caffeine Group 2]]===
 [[File:YLR426W-caffeine.png|700px]]
 ====Doubling Time at 0.25 concentration====

← Older revision		Revision as of 16:54, 3 May 2021
Line 144:		Line 144:

	[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]		[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]
		+
		+
		+	== Hydroxurea ==
		+	[[File:YLR.png\|center\|1000px\|]]
		+
		+	At first glance, the YLR426W strain seems to have a large difference in growth compared to the BY4735 strain. The YLR426W strain when exposed to hydroxyurea had an average doubling time of 946 minutes, while the BY4735 strain when stressed had a doubling time of 278 minutes. The YLR426W strain without stress had an average doubling time of 710 minutes, while the BY4735 strain unstressed had an average doubling time of 182 minutes. Through analysis, it seems the YLR426W strain just seems to grow slower than other strains.
		+
		+	[https://wiki.yeastgenome.org/index.php/UW-Stout/Hydroxyurea_SP21]

	==References==		==References==