YKL121W - Revision history

Teagueb: /* Caffeine Group 2 */

2021-05-05T15:05:29Z

‎Caffeine Group 2

Teagueb: /* Doubling Time at 0.25 concentration */

2021-05-05T12:18:05Z

‎Doubling Time at 0.25 concentration

Teagueb: /* Caffeine Group 2 */

2021-05-05T06:41:08Z

‎Caffeine Group 2

Teagueb: /* Caffeine Group 2 */

2021-05-04T23:10:57Z

‎Caffeine Group 2

Teagueb: /* Caffeine Group 2 */

2021-05-04T23:10:03Z

‎Caffeine Group 2

Teagueb: /* Hydroxyurea */

2021-05-03T17:18:41Z

‎Hydroxyurea

Teagueb: /* Ethanol Sensitivity */

2021-05-02T20:42:14Z

‎Ethanol Sensitivity

Teagueb: /* Ethanol Sensitivity */

2021-05-02T03:00:40Z

‎Ethanol Sensitivity

Teagueb: /* Ethanol Sensitivity */

2021-05-01T06:51:07Z

‎Ethanol Sensitivity

Teagueb: /* Ethanol Sensitivity */

2021-04-30T20:27:23Z

‎Ethanol Sensitivity

@@ Line 137: / Line 137: @@
 [[File:YKL121W graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; Td = (217-103) x ln(2)/ln(0.12/0.1) = 433 minutes The caffeine sped up the growth rate of YKL121W. The caffeine most likely effected the function of the p53 protein. This induced apoptosis.
+Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; YKL121W no caffeine= (185-73) x ln(2)/ln(.4/.1)= 56; Td = (217-103) x ln(2)/ln(0.12/0.1) = 433 minutes  The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function
 ===[[UW-Stout/Ethanol Sensitivity| Ethanol Sensitivity]]===

@@ Line 137: / Line 137: @@
 [[File:YKL121W graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; Td = (217-103) x ln(2)/ln(0.12/0.1) = 433 minutes
+Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; Td = (217-103) x ln(2)/ln(0.12/0.1) = 433 minutes The caffeine sped up the growth rate of YKL121W. The caffeine most likely effected the function of the p53 protein. This induced apoptosis.
 ===[[UW-Stout/Ethanol Sensitivity| Ethanol Sensitivity]]===

@@ Line 134: / Line 134: @@
 [[Image:IMG_1650.JPG|Pitri Dish YKL121W Yeast Strain]]
 ----
-===[[UW-Stout/Caffeine_SP21|Caffeine Group 2]]===
+===[[UW-Stout/Caffeine 2 SP211|Caffeine Group 2]]===
 [[File:YKL121W graph.png|700px]]
 ====Doubling Time at 0.25 concentration====

@@ Line 135: / Line 135: @@
 ----
 ===[[UW-Stout/Caffeine_SP21|Caffeine Group 2]]===
-[[File:Example.jpg]]
+[[File:YKL121W graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
 Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; Td = (217-103) x ln(2)/ln(0.12/0.1) = 433 minutes

@@ Line 135: / Line 135: @@
 ----
 ===[[UW-Stout/Caffeine_SP21|Caffeine Group 2]]===
-[[File:YKL121W-caffeine.png|700px]]
+[[File:Example.jpg]]
 ====Doubling Time at 0.25 concentration====
 Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes; Td = (217-103) x ln(2)/ln(0.12/0.1) = 433 minutes

← Older revision		Revision as of 17:18, 3 May 2021
Line 151:		Line 151:

	The YKL121W yeast strain had an average doubling time of 256 minutes, while the average doubling time of the BY4735 strain was 182 minutes. After being exposed to the stress hydroxyurea, the YKL121W strain had an average doubling time of 595 minutes, while the average doubling time of the stressed BY4725 strain was 278 minutes. After observing the results in different growth rates, it seems that the YKL121W strain is much more sensitive to hydroxyurea conditions.		The YKL121W yeast strain had an average doubling time of 256 minutes, while the average doubling time of the BY4735 strain was 182 minutes. After being exposed to the stress hydroxyurea, the YKL121W strain had an average doubling time of 595 minutes, while the average doubling time of the stressed BY4725 strain was 278 minutes. After observing the results in different growth rates, it seems that the YKL121W strain is much more sensitive to hydroxyurea conditions.
		+	[https://wiki.yeastgenome.org/index.php/UW-Stout/Hydroxyurea_SP21]

	==References==		==References==

← Older revision		Revision as of 20:42, 2 May 2021
Line 145:		Line 145:

	[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]		[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]
		+
		+
		+	== Hydroxyurea ==
		+	[[File:YKK121W Hydroxyurea.png\|center\|1000px\|]]
		+
		+	The YKL121W yeast strain had an average doubling time of 256 minutes, while the average doubling time of the BY4735 strain was 182 minutes. After being exposed to the stress hydroxyurea, the YKL121W strain had an average doubling time of 595 minutes, while the average doubling time of the stressed BY4725 strain was 278 minutes. After observing the results in different growth rates, it seems that the YKL121W strain is much more sensitive to hydroxyurea conditions.

	==References==		==References==

← Older revision		Revision as of 03:00, 2 May 2021
Line 143:		Line 143:

	The YKL121W yeast gene was determined to have an average doubling time of 257 minutes, which is a bit longer than the BY4735 strain, that has a doubling time of 182 minutes. The wild-type strain (BY4735) had very similar results regardless of whether ethanol was present. While the strain YKL121W without ethanol had greater cell growth compared to when the strain was exposed to 9% concentration of ethanol. Observing the difference in growth of YKL121W under different conditions, it is likely that this strain is more sensitive to ethanol. The doubling time of the BY4735 strain when exposed to ethanol is 218 minutes, and the doubling time of the YKL121W strain is 450 minutes, over double that of the wild-type strain.		The YKL121W yeast gene was determined to have an average doubling time of 257 minutes, which is a bit longer than the BY4735 strain, that has a doubling time of 182 minutes. The wild-type strain (BY4735) had very similar results regardless of whether ethanol was present. While the strain YKL121W without ethanol had greater cell growth compared to when the strain was exposed to 9% concentration of ethanol. Observing the difference in growth of YKL121W under different conditions, it is likely that this strain is more sensitive to ethanol. The doubling time of the BY4735 strain when exposed to ethanol is 218 minutes, and the doubling time of the YKL121W strain is 450 minutes, over double that of the wild-type strain.
		+
		+	[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]

	==References==		==References==

@@ Line 142: / Line 142: @@
 [[File:YKL121W Ethanol.png]]
-The YKL121W yeast gene was determined to have an average doubling time of 257 minutes, which is a bit longer than the BY4735 strain, that has a doubling time of 182 minutes. The wild-type strain (BY4735) had very similar results regardless of whether ethanol was present. While the strain YKL121W without ethanol had greater cell growth compared to when the strain was exposed to ethanol. Observing the difference in growth of YKL121W under different conditions, it is likely that this strain is more sensitive to ethanol. The doubling time of the BY4735 strain when exposed to ethanol is 218 minutes, and the doubling time of the YKL121W strain is 450 minutes, over double that of the wild-type strain.
+The YKL121W yeast gene was determined to have an average doubling time of 257 minutes, which is a bit longer than the BY4735 strain, that has a doubling time of 182 minutes. The wild-type strain (BY4735) had very similar results regardless of whether ethanol was present. While the strain YKL121W without ethanol had greater cell growth compared to when the strain was exposed to 9% concentration of ethanol. Observing the difference in growth of YKL121W under different conditions, it is likely that this strain is more sensitive to ethanol. The doubling time of the BY4735 strain when exposed to ethanol is 218 minutes, and the doubling time of the YKL121W strain is 450 minutes, over double that of the wild-type strain.
 ==References==