YJL133C-A - Revision history

Teagueb at 01:48, 21 December 2022

2022-12-21T01:48:05Z

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:43:00Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:14:25Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:09:39Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:08:52Z

‎Hydrogen Peroxide

Teagueb at 21:02, 19 December 2022

2022-12-19T21:02:12Z

Teagueb: /* Community Commentary */

2022-12-17T16:53:43Z

‎Community Commentary

Teagueb: /* Ethanol */

2022-12-17T16:24:41Z

‎Ethanol

Teagueb: /* Analysis/Conclusion */

2022-12-17T00:21:32Z

‎Analysis/Conclusion

Teagueb: /* Community Commentary */

2022-12-17T00:11:19Z

‎Community Commentary

← Older revision		Revision as of 01:48, 21 December 2022
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	===Analysis/Conclusion===		===Analysis/Conclusion===
	Trial 1 and trial 3 of the glycerol torture experiment performed on the knockout on the YJL133C-A in yeast did not look incredibly promising- it is hard to rely on these results and draw any conclusions. The main pattern that seems to be apparent is that the yeast is not reproducing at a consistent rate when grown in glycerol without this gene functioning. A number of different factors could have gone wrong for these results to turn out this way, so it is hard to tell what happened! Disregarding trial 1 and trial 3, the data in trial 3 seemed promising! It is clear that the yeast was growing at a much slower rate, with less than half of a doubling time than what appeared in the negative control. I would definitely say that growing yeast in a glycerol media without YJL133C-A functioning puts a significant amount of stress on the organism.		Trial 1 and trial 3 of the glycerol torture experiment performed on the knockout on the YJL133C-A in yeast did not look incredibly promising- it is hard to rely on these results and draw any conclusions. The main pattern that seems to be apparent is that the yeast is not reproducing at a consistent rate when grown in glycerol without this gene functioning. A number of different factors could have gone wrong for these results to turn out this way, so it is hard to tell what happened! Disregarding trial 1 and trial 3, the data in trial 3 seemed promising! It is clear that the yeast was growing at a much slower rate, with less than half of a doubling time than what appeared in the negative control. I would definitely say that growing yeast in a glycerol media without YJL133C-A functioning puts a significant amount of stress on the organism.
		+
		+	==UW Stout/FA 2022 Bud Scars==
		+	Cells that had the gene YJL133C-A had an average of 1.06 scars per cell. Out of 100 cells counted, 106 bud scars were counted. The wild yeast BY4735 had an average of 2.46 bud scars per cell (100 cells counted, 246 bud scars). This indicates that the knocked-out gene significantly slowed down the reproduction rate of these cells.
		+	[[File:Yeast_1(2)_2_67.png]]
		+	''picture does not include all cells that were counted and added for visual of bud scars''

@@ Line 66: / Line 66: @@
 ===Hydrogen Peroxide===
-[[Image:1.jpg|center|thumb|600px|YJL133C-A]]
+[[Image:1.jpg|center|thumb|600px|The x-axis is time in minutes and the y-axis is OD600]]
 This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]].

← Older revision		Revision as of 21:14, 19 December 2022
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	===Hydrogen Peroxide===		===Hydrogen Peroxide===
		+	[[Image:1.jpg\|center\|thumb\|600px\|YJL133C-A]]
		+
	This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]].		This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]].

← Older revision		Revision as of 21:09, 19 December 2022
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	===Hydrogen Peroxide===		===Hydrogen Peroxide===
−	This is the link to the protocol page:[[UW-Stout/Hydrogen Peroxide FA22]].	+	This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]].
		+
		+	The doubling time for YJL133C-A under the stress of hydrogen peroxide is 306 minutes whereas without stress it was only 206 minutes. As shown in the chart above, the optical density of the YJL133C-A is very similar to that of the wild type yeast. The BY4375 under stress had a 598 minute doubling time and with no stress there was a 229 minute doubling time. Even though the stress placed on the YJL133C-A yeast, resulted in an increased doubling time, this was consistent with the wild type yeast.

← Older revision		Revision as of 21:08, 19 December 2022
Line 66:		Line 66:

	===Hydrogen Peroxide===		===Hydrogen Peroxide===
		+	This is the link to the protocol page:[[UW-Stout/Hydrogen Peroxide FA22]].

← Older revision		Revision as of 21:02, 19 December 2022
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	As there was no significant difference between the wild-type yeast exposed to UV and the trial strains, we can conclude that the gene is not used in the defense against UV damage nor the repair of UV damage in yeast.		As there was no significant difference between the wild-type yeast exposed to UV and the trial strains, we can conclude that the gene is not used in the defense against UV damage nor the repair of UV damage in yeast.
		+
		+	===Hydrogen Peroxide===

@@ Line 46: / Line 46: @@
 [[Image:Best Ethanol KO graph.png|Graph|center|700px|YJL133C-A]]
-YJL133C-A KO gene
+YJL133C-A's line lies in the middle of the overall growth curve. The doubling time is 67.78. These cells went not affected but the Ethanol.
 ===[[UW-Stout/Ultraviolet_Light_FA22|UV Light]]===

← Older revision		Revision as of 00:21, 17 December 2022
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	===Analysis/Conclusion===		===Analysis/Conclusion===
		+	Trial 1 and trial 3 of the glycerol torture experiment performed on the knockout on the YJL133C-A in yeast did not look incredibly promising- it is hard to rely on these results and draw any conclusions. The main pattern that seems to be apparent is that the yeast is not reproducing at a consistent rate when grown in glycerol without this gene functioning. A number of different factors could have gone wrong for these results to turn out this way, so it is hard to tell what happened! Disregarding trial 1 and trial 3, the data in trial 3 seemed promising! It is clear that the yeast was growing at a much slower rate, with less than half of a doubling time than what appeared in the negative control. I would definitely say that growing yeast in a glycerol media without YJL133C-A functioning puts a significant amount of stress on the organism.

@@ Line 36: / Line 36: @@
 [[Image:Knockout_v._caffeine_trial1_Viv.png|100pxs|Trial 1]]
-[[Image:Knockout_gene_v._Caffeine_trial_2.png|100pxs|Trial 2]]
+[[Image:Knockout_gene_v._Caffeine_trial_2.png|600px|Trial 2]]
 ====Interpretation====