YHL029C - Revision history

Teagueb: /* Community Commentary */

2022-05-12T03:35:12Z

‎Community Commentary

2022-05-12T03:29:49Z

‎Results

2022-05-12T03:11:13Z

‎Interpretation

2022-05-12T02:56:39Z

‎Interpretation

2022-05-12T02:30:58Z

‎Community Commentary

2022-05-09T22:30:50Z

‎UW Stout/Nystatin SP22

2022-05-09T22:28:25Z

‎Results

2022-05-09T17:11:01Z

‎UW-Stout/Nystatin SP22

2022-05-09T03:37:37Z

‎Community Commentary

2022-05-08T17:39:03Z

← Older revision		Revision as of 03:35, 12 May 2022
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	[[File:029C-T2.jpg]]		[[File:029C-T2.jpg]]
	From the growth curve, we can see that the YHL029C control grows at a higher rate than the wild type control and experimental, but the treated YHL029C grows at a lower rate than the wild type yeast, meaning that the removal of the YHL029C gene lowers resistance to Nystatin.		From the growth curve, we can see that the YHL029C control grows at a higher rate than the wild type control and experimental, but the treated YHL029C grows at a lower rate than the wild type yeast, meaning that the removal of the YHL029C gene lowers resistance to Nystatin.
		+
		+
		+	===[[UW-Stout/Formamide SP22]]===

@@ Line 174: / Line 174: @@
 ===Results===
-[[File:wildyeast.jpg|400px|]] [[File:trial222.jpg|400px|]] [[File:doublingtimestable.jpg|400px|]]
+[[File:wildyeast.jpg|400px|]] [[File:yeast.jpg|400px|]] [[File:doublingtimestable.jpg|400px|]]
@@ Line 180: / Line 180: @@
 *Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
 *Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)
 ===Interpretation===

@@ Line 183: / Line 183: @@
 ===Interpretation===
-The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YHL029C strain. The YHL029C strain had doubling times significantly faster than the wild yeast cell, with a doubling time of 637.92 min. It can be concluded that the transformed yeast cell, YHL029C, had a unique response to the formamide stress test used in the experiment.
+The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YHL029C strain. The YHL029C strain had doubling times significantly faster than the wild yeast cell, with the fastest doubling time of 566.06 min. It can be concluded that the transformed yeast cell, YHL029C, had a unique response to the formamide stress test used in the experiment.
 ==References==

← Older revision		Revision as of 02:56, 12 May 2022
Line 183:		Line 183:

	===Interpretation===		===Interpretation===
		+	The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YHL029C strain. The YHL029C strain had doubling times significantly faster than the wild yeast cell, with a doubling time of 637.92 min. It can be concluded that the transformed yeast cell, YHL029C, had a unique response to the formamide stress test used in the experiment.

	==References==		==References==

@@ Line 170: / Line 170: @@
 [[File:029C-T2.jpg]]
 From the growth curve, we can see that the YHL029C control grows at a higher rate than the wild type control and experimental, but the treated YHL029C grows at a lower rate than the wild type yeast, meaning that the removal of the YHL029C gene lowers resistance to Nystatin.
 ==References==

@@ Line 168: / Line 168: @@
 ====Results====
 [[File:YHL-T1.jpg]]
-[[File:YHL-T2.jpg]]
+[[File:029C-T2.jpg]]
 ==References==

@@ Line 164: / Line 164: @@
 Based on the growth curve, YHL029C displayed inhibition extremely identical to wild type yeast cells, and the range for both controls had a strong correlation. This suggests that the knockout may have had very little affect on oxidative stress response.
-===[[UW-Stout/Nystatin SP22]]===
+===[[UW Stout/Nystatin SP22]]===
 As part of the University of Wisconsin Stout [[UW-Stout/About|Orphan Gene Project]] this gene was tested by exposing the cells to 1µg/ml Nystatin solution.
 ====Results====

← Older revision		Revision as of 03:37, 9 May 2022
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	Based on the growth curve, YHL029C displayed inhibition extremely identical to wild type yeast cells, and the range for both controls had a strong correlation. This suggests that the knockout may have had very little affect on oxidative stress response.		Based on the growth curve, YHL029C displayed inhibition extremely identical to wild type yeast cells, and the range for both controls had a strong correlation. This suggests that the knockout may have had very little affect on oxidative stress response.
		+	===[[UW-Stout/Nystatin SP22]]===
		+	As part of the University of Wisconsin Stout [[UW-Stout/About\|Orphan Gene Project]] this gene was tested by exposing the cells to 1µg/ml Nystatin solution.
		+	====Results====
		+	[[File:YHL-T1.jpg]]
		+	[[File:YHL-T2.jpg]]

	==References==		==References==

← Older revision		Revision as of 17:39, 8 May 2022
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	-We can then conclude that the knocked-out gene in YHL029C doesn't have a significant impact on the growth of the yeast cell.		-We can then conclude that the knocked-out gene in YHL029C doesn't have a significant impact on the growth of the yeast cell.
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		+
		+	'''[[UW-Stout/pH Acid SP22]]'''
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		+	[[File:Strain4.jpg\|350px\|]]
		+	Knocked out gene seems to have some effect on cell growth in pH 7 environment based on the flattening of the growth curve. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve. Please see protocol for specific quantitative doubling time results.
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		+
		+	[[File:Wild (1).jpg\|350px\|]]
		+	The wild-type strain was used as a control in this experiment; no genes were knocked out, it just represents how the experiment ran on the knocked-out gene strains affects a "typical" cell growth curve. Inclusion of this data on each knock-out strain growth curve graph made for difficult interpretation, so it has been separated. Please refer to this graph as a control when viewing the knock-out strain growth curve graph. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve.