YGR079W - Revision history

Teagueb: /* Community Commentary */

2022-05-12T03:35:32Z

‎Community Commentary

Teagueb: /* Results */

2022-05-12T03:30:11Z

‎Results

Teagueb: /* Interpretation */

2022-05-12T03:13:10Z

‎Interpretation

Teagueb: /* Interpretation */

2022-05-12T02:54:47Z

‎Interpretation

Teagueb: /* Community Commentary */

2022-05-12T02:30:34Z

‎Community Commentary

Teagueb: /* UW-Stout/Nystatin SP22 */

2022-05-09T17:11:27Z

‎UW-Stout/Nystatin SP22

Teagueb: /* UW-Stout/Nystatin SP22 */

2022-05-09T04:58:17Z

‎UW-Stout/Nystatin SP22

Teagueb: /* [UW-Stout/Nystatin SP22]] */

2022-05-09T04:12:06Z

‎[UW-Stout/Nystatin SP22]]

Teagueb: /* Community Commentary */

2022-05-09T03:39:49Z

‎Community Commentary

Teagueb at 17:44, 8 May 2022

2022-05-08T17:44:15Z

← Older revision		Revision as of 03:35, 12 May 2022
Line 180:		Line 180:

	Based on the growth curve, we can see that the YGR079W strain is more slowly growing than the wild type yeast. The difference between the control and experimental in the wild type yeast and the YGR079W strain yeast is very similar. This indicates that the removal of the YGR079W gene has no effect on the cells resistance to Nystatin.		Based on the growth curve, we can see that the YGR079W strain is more slowly growing than the wild type yeast. The difference between the control and experimental in the wild type yeast and the YGR079W strain yeast is very similar. This indicates that the removal of the YGR079W gene has no effect on the cells resistance to Nystatin.
		+
		+
		+	===[[UW-Stout/Formamide SP22]]===

@@ Line 184: / Line 184: @@
 ===Results===
-[[File:wildyeast.jpg|400px|]] [[File:trial222.jpg|400px|]] [[File:doublingtimestable.jpg|400px|]]
+[[File:wildyeast.jpg|400px|]] [[File:yeast.jpg|400px|]] [[File:doublingtimestable.jpg|400px|]]
@@ Line 190: / Line 190: @@
 *Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
 *Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)
 ===Interpretation===

@@ Line 193: / Line 193: @@
 ===Interpretation===
-The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YGR079W strain. The YDR307W strain had doubling times significantly slower than the wild yeast cell, with a doubling time of 2756.8 min. It can be concluded that the transformed yeast cell, YGR079W, had a unique response to the formamide stress test used in the experiment.
+The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YGR079W strain. The YGr079W strain had doubling times significantly slower than the wild yeast cell, with a doubling time of 2756.8 min. It can be concluded that the transformed yeast cell, YGR079W, had a unique response to the formamide stress test used in the experiment.
 ==References==

← Older revision		Revision as of 02:54, 12 May 2022
Line 193:		Line 193:

	===Interpretation===		===Interpretation===
		+	The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YGR079W strain. The YDR307W strain had doubling times significantly slower than the wild yeast cell, with a doubling time of 2756.8 min. It can be concluded that the transformed yeast cell, YGR079W, had a unique response to the formamide stress test used in the experiment.

	==References==		==References==

← Older revision		Revision as of 02:30, 12 May 2022
Line 180:		Line 180:

	Based on the growth curve, we can see that the YGR079W strain is more slowly growing than the wild type yeast. The difference between the control and experimental in the wild type yeast and the YGR079W strain yeast is very similar. This indicates that the removal of the YGR079W gene has no effect on the cells resistance to Nystatin.		Based on the growth curve, we can see that the YGR079W strain is more slowly growing than the wild type yeast. The difference between the control and experimental in the wild type yeast and the YGR079W strain yeast is very similar. This indicates that the removal of the YGR079W gene has no effect on the cells resistance to Nystatin.
		+
		+
		+
		+	===Results===
		+	[[File:wildyeast.jpg\|400px\|]] [[File:trial222.jpg\|400px\|]] [[File:doublingtimestable.jpg\|400px\|]]
		+
		+
		+	*3% formamide line graph: x-axis= growing time (min); y-axis= optical density 600
		+	*Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
		+	*Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)
		+
		+
		+	===Interpretation===

	==References==		==References==

@@ Line 173: / Line 173: @@
 Based on the growth curve, YGR079W demonstrated close to standard inhibition under the stress of the hydrogen peroxide solution when compared to wild type cells. It's control exhibited slow growth but ended around the same value as wild type cells.
-===[[UW-Stout/Nystatin SP22]]===
+===[[UW Stout/Nystatin SP22]]===
 As part of the University of Wisconsin Stout [[UW-Stout/About|Orphan Gene Project]] this gene was tested by exposing the cells to 1µg/ml Nystatin solution.
 ====Results====

@@ Line 178: / Line 178: @@
 [[File:YGR-T1.jpg]]
 [[File:YGR-T2.jpg]]
 ==References==

← Older revision		Revision as of 03:39, 9 May 2022
Line 173:		Line 173:

	Based on the growth curve, YGR079W demonstrated close to standard inhibition under the stress of the hydrogen peroxide solution when compared to wild type cells. It's control exhibited slow growth but ended around the same value as wild type cells.		Based on the growth curve, YGR079W demonstrated close to standard inhibition under the stress of the hydrogen peroxide solution when compared to wild type cells. It's control exhibited slow growth but ended around the same value as wild type cells.
		+	===[UW-Stout/Nystatin SP22]]===
		+	As part of the University of Wisconsin Stout [[UW-Stout/About\|Orphan Gene Project]] this gene was tested by exposing the cells to 1µg/ml Nystatin solution.
		+	====Results====
		+	[[File:YGR-T1.jpg]]
		+	[[File:YGR-T2.jpg]]

	==References==		==References==

← Older revision		Revision as of 17:44, 8 May 2022
Line 190:		Line 190:

	-We can then conclude that the knocked-out gene in YGR079W doesn't have a significant impact on the growth of the yeast cell.		-We can then conclude that the knocked-out gene in YGR079W doesn't have a significant impact on the growth of the yeast cell.
		+
		+
		+	'''[[UW-Stout/pH Acid SP22]]'''
		+
		+	[[File:Strain8.jpg\|350px\|]]
		+	Knocked out gene seems to have no effect on cell growth in pH 7 environment based on the nearly identical natural and stressed growth curves. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve. Please see protocol for specific quantitative doubling time results.
		+
		+
		+	[[File:Wild (1).jpg\|350px\|]]
		+	The wild-type strain was used as a control in this experiment; no genes were knocked out, it just represents how the experiment ran on the knocked-out gene strains affects a "typical" cell growth curve. Inclusion of this data on each knock-out strain growth curve graph made for difficult interpretation, so it has been separated. Please refer to this graph as a control when viewing the knock-out strain growth curve graph. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve.