YGL146C - Revision history

Teagueb: /* Community Commentary */

2022-05-12T03:35:51Z

‎Community Commentary

2022-05-12T03:30:30Z

‎Results

2022-05-12T03:14:22Z

‎Interpretation

2022-05-12T02:52:33Z

‎Interpretation

2022-05-12T02:29:58Z

‎Community Commentary

2022-05-10T01:35:29Z

‎Results

2022-05-10T00:28:09Z

‎UW Stout/Nystatin SP22

2022-05-09T17:11:54Z

‎UW-Stout/Nystatin SP22

2022-05-09T03:51:33Z

‎UW-Stout/Nystatin

2022-05-09T03:49:35Z

‎Community Commentary

← Older revision		Revision as of 03:35, 12 May 2022
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	The growth rates of the experimental and control YGL146C strain, when compared to that of the wild type yeast, were similar enough not to indicate a difference in Nystatin tolerance when the YGL146C gene was knocked out.		The growth rates of the experimental and control YGL146C strain, when compared to that of the wild type yeast, were similar enough not to indicate a difference in Nystatin tolerance when the YGL146C gene was knocked out.
		+
		+
		+	===[[UW-Stout/Formamide SP22]]===

@@ Line 187: / Line 187: @@
 ===Results===
-[[File:wildyeast.jpg|400px|]] [[File:trial222.jpg|400px|]] [[File:doublingtimestable.jpg|400px|]]
+[[File:wildyeast.jpg|400px|]] [[File:yeast.jpg|400px|]] [[File:doublingtimestable.jpg|400px|]]
@@ Line 193: / Line 193: @@
 *Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
 *Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)
 ===Interpretation===

@@ Line 196: / Line 196: @@
 ===Interpretation===
-The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YGL146C strain. The YDR307W strain had doubling times significantly faster than the wild yeast cell, with a doubling time of 599.04 min. It can be concluded that the transformed yeast cell, YGL146C, had a unique response to the formamide stress test used in the experiment.
+The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YGL146C strain. The YGL146C strain had doubling times significantly faster than the wild yeast cell, with a doubling time of 599.04 min. It can be concluded that the transformed yeast cell, YGL146C, had a unique response to the formamide stress test used in the experiment.
 ==References==

← Older revision		Revision as of 02:52, 12 May 2022
Line 196:		Line 196:

	===Interpretation===		===Interpretation===
		+	The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YGL146C strain. The YDR307W strain had doubling times significantly faster than the wild yeast cell, with a doubling time of 599.04 min. It can be concluded that the transformed yeast cell, YGL146C, had a unique response to the formamide stress test used in the experiment.

	==References==		==References==

← Older revision		Revision as of 02:29, 12 May 2022
Line 183:		Line 183:

	The growth rates of the experimental and control YGL146C strain, when compared to that of the wild type yeast, were similar enough not to indicate a difference in Nystatin tolerance when the YGL146C gene was knocked out.		The growth rates of the experimental and control YGL146C strain, when compared to that of the wild type yeast, were similar enough not to indicate a difference in Nystatin tolerance when the YGL146C gene was knocked out.
		+
		+
		+
		+	===Results===
		+	[[File:wildyeast.jpg\|400px\|]] [[File:trial222.jpg\|400px\|]] [[File:doublingtimestable.jpg\|400px\|]]
		+
		+
		+	*3% formamide line graph: x-axis= growing time (min); y-axis= optical density 600
		+	*Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
		+	*Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)
		+
		+
		+	===Interpretation===

	==References==		==References==

← Older revision		Revision as of 01:35, 10 May 2022
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	[[File:YGL-T2.jpg]]		[[File:YGL-T2.jpg]]
		+
		+	The growth rates of the experimental and control YGL146C strain, when compared to that of the wild type yeast, were similar enough not to indicate a difference in Nystatin tolerance when the YGL146C gene was knocked out.

	==References==		==References==

@@ Line 177: / Line 177: @@
 As part of the University of Wisconsin Stout [[UW-Stout/About|Orphan Gene Project]] this gene was tested by exposing the cells to 1µg/ml Nystatin solution.
 ====Results====
 ==References==

@@ Line 174: / Line 174: @@
 [[File:Wild.jpg|350px|]]
 The wild-type strain was used as a control in this experiment; no genes were knocked out, it just represents how the experiment ran on the knocked-out gene strains affects a "typical" cell growth curve. Inclusion of this data on each knock-out strain growth curve graph made for difficult interpretation, so it has been separated. Please refer to this graph as a control when viewing the knock-out strain growth curve graph. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve.
-===[[UW-Stout/Nystatin SP22]]===
+===[[UW Stout/Nystatin SP22]]===
 As part of the University of Wisconsin Stout [[UW-Stout/About|Orphan Gene Project]] this gene was tested by exposing the cells to 1µg/ml Nystatin solution.
 ====Results====