YGL138C - Revision history

Teagueb: /* Doubling Time at 0.25 concentration */

2021-05-05T15:07:58Z

‎Doubling Time at 0.25 concentration

2021-05-05T12:15:42Z

‎Caffeine Group 2

2021-05-05T06:40:29Z

‎Caffeine Group 2

2021-05-04T23:09:35Z

‎Caffeine Group 2

2021-05-04T23:08:07Z

‎Caffeine Group 2

2021-05-04T23:07:17Z

‎Caffeine Group 2

2021-05-03T17:15:52Z

‎Hyrdoxyurea

2021-05-03T17:15:06Z

‎Ethanol Sensitivity

2021-05-02T03:13:29Z

2021-04-29T04:05:19Z

‎Caffeine Group 2

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 [[File:YGL138C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-YGL138C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes; Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes These doubling times show that the 0.25 mM concentration in YGL138C slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
+YGL138C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes; YGL138C Td= (153-33) x ln(2)/ln(.7/.1)= 42 minutes; Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes These doubling times show that the 0.25 mM concentration in YGL138C slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
 ===[[UW-Stout/Ethanol Sensitivity| Ethanol Sensitivity]]===

@@ Line 134: / Line 134: @@
 [[File:YGL138C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
-YGL138C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes; Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes
+YGL138C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes; Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes These doubling times show that the 0.25 mM concentration in YGL138C slowed down the rate of growth. The caffeine was able to slow down metabolic activity. Caffeine may effect the function of the Rad52 protein that aids in DNA double-stranded breaks and maintaining genome stability. The caffeine may also aid in the process of the cell protecting itself from reacting oxygen species. Since the concentration is small in volume, apoptosis was not induced for cell function.
 ===[[UW-Stout/Ethanol Sensitivity| Ethanol Sensitivity]]===

@@ Line 131: / Line 131: @@
 The yeast strain YGL138C was impacted significantly by the heat shocking of the thermal cycler. As seen in the image, the controlled plate grew a lot of small DNA colonies all throughout the majority of pitri dish. It looks like a very thick sheet of cells. When compared to the two trial heat shocking plates, there are a lot less cells being grown. The trial cells are slightly larger than those of the controlled, but not by much. The quantity of the cells is much smaller, and the quality (size) of the cells is not that much bigger than what they're being compared to. This gene was heat sensitive, but not as much as the YLR426W yeast strain.
 ----
-===[[UW-Stout/Caffeine_SP21|Caffeine Group 2]]===
+===[[UW-Stout/Caffeine 2 SP21|Caffeine Group 2]]===
 [[File:YGL138C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====

@@ Line 132: / Line 132: @@
 ----
 ===[[UW-Stout/Caffeine_SP21|Caffeine Group 2]]===
-[[File:YGL138C.png|700px]]
+[[File:YGL138C graph.png|700px]]
 ====Doubling Time at 0.25 concentration====
 YGL138C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes; Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes

@@ Line 132: / Line 132: @@
 ----
 ===[[UW-Stout/Caffeine_SP21|Caffeine Group 2]]===
-[[File:Example.jpg]]
+[[File:YGL138C.png|700px]]
 ====Doubling Time at 0.25 concentration====
 YGL138C Td = (217-1) x ln(2)/ln(0.2/0.1) = 216 minutes; Wild Type Td = (146-66) x ln(2)/ln(.6/.2) = 88 minutes

@@ Line 148: / Line 148: @@
 [[File:YGL.png|center|1000px|]]
-The YGL138X strain had an average doubling time that was very similar to the growth rate of the BY4735 strain. The stressed YGL138C strain had a doubling time of 286 minutes, while the average doubling time of the stressed BY4735 strain had an average doubling time of 278 minutes. What is similar in both unstressed samples is what is interesting, the unstressed YGL138C strain had an average doubling time of 167 minutes, while the BY4735 unstressed strain had an average doubling time of 182 minutes. This means the stressor had little effect on the way the rate of growth of the strain.
+The YGL138X strain had an average doubling time that was very similar to the growth rate of the BY4735 strain. The stressed YGL138C strain had a doubling time of 286 minutes, while the average doubling time of the stressed BY4735 strain had an average doubling time of 278 minutes. What is similar in both unstressed samples is what is interesting, the unstressed YGL138C strain had an average doubling time of 167 minutes, while the BY4735 unstressed strain had an average doubling time of 182 minutes. This means the stressor had little effect on the rate of growth of the strain.
 ==References==

← Older revision		Revision as of 17:15, 3 May 2021
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	[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]		[[https://wiki.yeastgenome.org/index.php/UW-Stout/Ethanol_SP21]]
		+
		+
		+
		+	== Hyrdoxyurea ==
		+	[[File:YGL.png\|center\|1000px\|]]
		+
		+	The YGL138X strain had an average doubling time that was very similar to the growth rate of the BY4735 strain. The stressed YGL138C strain had a doubling time of 286 minutes, while the average doubling time of the stressed BY4735 strain had an average doubling time of 278 minutes. What is similar in both unstressed samples is what is interesting, the unstressed YGL138C strain had an average doubling time of 167 minutes, while the BY4735 unstressed strain had an average doubling time of 182 minutes. This means the stressor had little effect on the way the rate of growth of the strain.

	==References==		==References==