YFL064C - Revision history

Teagueb: /* Community Commentary */

2019-05-10T02:50:33Z

‎Community Commentary

2019-05-10T00:58:29Z

‎Cold Shock Sensitivity

2019-05-10T00:33:43Z

‎Cold Shock Sensitivity

2019-05-10T00:20:39Z

‎Community Commentary

2019-05-09T21:09:14Z

2019-05-07T15:45:37Z

‎Heat Shock

2019-05-06T21:20:05Z

‎Caffeine Sensitivity

2019-05-06T21:18:23Z

2019-05-06T17:44:49Z

‎Community Commentary

2019-05-06T00:49:22Z

‎Community Commentary

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+YFL064C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YFL064C.
 ==References==
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← Older revision		Revision as of 00:58, 10 May 2019
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	===Cold Shock Sensitivity===		===Cold Shock Sensitivity===
	[[File:YFL064CBB.png]]		[[File:YFL064CBB.png]]
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		+	Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock Cold Shock]
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 ===Cold Shock Sensitivity===
 [[File:YFL064CBB.png]]
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 [[File:pH-YFL064C.PNG]]
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 [[File:YFL064C.jpg|YFL064C VS BY4735]]
   Protocol above, results show very different initial reactions, pace of growth very different, but ended at about the same pace.
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← Older revision		Revision as of 15:45, 7 May 2019
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	[[File:YFL064C Heat Shock.PNG]]		[[File:YFL064C Heat Shock.PNG]]

		+	Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.

	===Stressing with Hydroxyurea===		===Stressing with Hydroxyurea===

← Older revision		Revision as of 17:44, 6 May 2019
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	[[File:YFL064C_sensitivity_Methanol.png]]		[[File:YFL064C_sensitivity_Methanol.png]]
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		+	===Heat Shock===
		+	[[File:YFL064C Heat Shock.PNG]]
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	===Stressing with Hydroxyurea===		===Stressing with Hydroxyurea===

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 In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase.  In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes.  However, the knock-out strain did reach a substantially higher OD600 before saturating.
-===G-418 Stress===
+===[[UW-Stout/G-418 | G-418 Stress]]===
 [[File:image007.png]]