YER186C - Revision history

Teagueb at 01:40, 21 December 2022

2022-12-21T01:40:09Z

Teagueb at 01:39, 21 December 2022

2022-12-21T01:39:52Z

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:41:49Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:26:24Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:22:44Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:13:01Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:12:06Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:11:52Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:11:09Z

‎Hydrogen Peroxide

Teagueb at 20:59, 19 December 2022

2022-12-19T20:59:58Z

@@ Line 104: / Line 104: @@
 ==UW Stout/FA 2022 Bud Scars==
 Cells that had the gene YER186C had an average of 2.82 scars per cell. Out of 100 cells counted, 282 bud scars were counted. The wild yeast BY4735 had an average of 2.46 bud scars per cell (100 cells counted, 246 bud scars). This indicates that the knocked-out gene slightly sped up the reproduction rate of these cells.
-[[File:Yeast_52(2)_7_8.png]]
+[[File:Yeast_2(2)_7_8.png]]
 ''picture does not include all cells that were counted and added for a visual of bud scars''

← Older revision		Revision as of 01:39, 21 December 2022
Line 101:		Line 101:
	===Analysis/Conclusion===		===Analysis/Conclusion===
	The results from glycerol torture on the YER186C knockout were quite interesting. All 3 graphs looked ridiculous, but there is one point that is clear: the yeast cells did not grow very successfully in the glycerol media without this gene. Trial 1's data was all around a little scattered and the jump near the end was a bit concerning considering it happened in every single experiment- so we weren't very confident in the results. However, in Trial 2 it continued to be weird... The OD600 reading did not seem to go up at all! This could mean the cells are all dead or not reproducing. This is intriguing data! The negative control graph for this trial even looked normal which leads us to believe that the flatlined data wasn't just a slip up. Trial 3, like trial 1, also seemed like a bust. It was interesting that the data showed a significant decrease in the original cells, but one thing is for certain: the yeast was definitely not reproducing and was most likely dead.		The results from glycerol torture on the YER186C knockout were quite interesting. All 3 graphs looked ridiculous, but there is one point that is clear: the yeast cells did not grow very successfully in the glycerol media without this gene. Trial 1's data was all around a little scattered and the jump near the end was a bit concerning considering it happened in every single experiment- so we weren't very confident in the results. However, in Trial 2 it continued to be weird... The OD600 reading did not seem to go up at all! This could mean the cells are all dead or not reproducing. This is intriguing data! The negative control graph for this trial even looked normal which leads us to believe that the flatlined data wasn't just a slip up. Trial 3, like trial 1, also seemed like a bust. It was interesting that the data showed a significant decrease in the original cells, but one thing is for certain: the yeast was definitely not reproducing and was most likely dead.
		+
		+	==UW Stout/FA 2022 Bud Scars==
		+	Cells that had the gene YER186C had an average of 2.82 scars per cell. Out of 100 cells counted, 282 bud scars were counted. The wild yeast BY4735 had an average of 2.46 bud scars per cell (100 cells counted, 246 bud scars). This indicates that the knocked-out gene slightly sped up the reproduction rate of these cells.
		+	[[File:Yeast_52(2)_7_8.png]]
		+	''picture does not include all cells that were counted and added for a visual of bud scars''

@@ Line 57: / Line 57: @@
 ===Hydrogen Peroxide===
-[[Image:YER186C.png|center|thumb|600px|YER186C]]
+[[Image:YER186C.png|center|thumb|600px|The x-axis is time in minutes and the y-axis is OD600]]
 This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]].

@@ Line 58: / Line 58: @@
 ===Hydrogen Peroxide===
 [[Image:YER186C.png|center|thumb|600px|YER186C]]
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
 <!--

@@ Line 57: / Line 57: @@
 ===Hydrogen Peroxide===
+[[Image:YER186C.png|center|thumb|600px|YER186C]]
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
 <!--

@@ Line 55: / Line 55: @@
 [[Image:Best Ethanol KO graph.png|Graph|center|700px|YJL133C-A]]
 YER186C is our outlier for this Ethanol experiment. The Ethanol dramatically decreased cell growth. The doubling time is 189.60
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->