YER076C - Revision history

Teagueb at 01:41, 21 December 2022

2022-12-21T01:41:01Z

Teagueb at 01:36, 21 December 2022

2022-12-21T01:36:04Z

Teagueb at 01:34, 21 December 2022

2022-12-21T01:34:39Z

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:40:59Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:30:33Z

‎Hydrogen Peroxide

Teagueb: /* Hydrogen Peroxide */

2022-12-19T21:23:15Z

‎Hydrogen Peroxide

Teagueb at 21:21, 19 December 2022

2022-12-19T21:21:41Z

Teagueb: /* Community Commentary */

2022-12-19T20:58:41Z

‎Community Commentary

Teagueb: /* Ethanol */

2022-12-17T16:49:04Z

‎Ethanol

Teagueb: /* Ethanol */

2022-12-17T16:42:06Z

‎Ethanol

@@ Line 94: / Line 94: @@
 Cells that had the gene YER076C had an average of 1.46 scars per cell. Out of 100 cells counted, 146 bud scars were counted. The wild yeast BY4735 had an average of 2.46 bud scars per cell (100 cells counted, 246 bud scars). This indicates that the knocked-out gene significantly slowed down the reproduction rate of these cells.
-[[File:Yeast_2(2)_7_8.png]]
+[[File:Yeast_3(2)_2_53.png]]
 ''picture does not include all cells that were counted and added for a visual of bud scars''

@@ Line 94: / Line 94: @@
 Cells that had the gene YER076C had an average of 1.46 scars per cell. Out of 100 cells counted, 146 bud scars were counted. The wild yeast BY4735 had an average of 2.46 bud scars per cell (100 cells counted, 246 bud scars). This indicates that the knocked-out gene significantly slowed down the reproduction rate of these cells.
-[[File:Yeast_2(2)_7_8]]
+[[File:Yeast_2(2)_7_8.png]]
 ''picture does not include all cells that were counted and added for a visual of bud scars''

← Older revision		Revision as of 01:34, 21 December 2022
Line 90:		Line 90:
	===Analysis/Conclusion===		===Analysis/Conclusion===
	Our results for our glycerol media is not consistent, the first trial was messed up, and the second and third trial have a large difference. Trials 2 & 3 bring up some issues because trial 2's time is lower than the negative control which would mean that this knockout gene strain of yeast would work better in the glycerol media than the glucose media, but our time for trial 3 is much larger than the negative control, and because our first trial was inconclusive we would need to run another trial to figure out whether or not this strain would truly live better in the glycerol media or not.		Our results for our glycerol media is not consistent, the first trial was messed up, and the second and third trial have a large difference. Trials 2 & 3 bring up some issues because trial 2's time is lower than the negative control which would mean that this knockout gene strain of yeast would work better in the glycerol media than the glucose media, but our time for trial 3 is much larger than the negative control, and because our first trial was inconclusive we would need to run another trial to figure out whether or not this strain would truly live better in the glycerol media or not.
		+
		+	=UW Stout/FA 2022 Bud Scars=
		+	Cells that had the gene YER076C had an average of 1.46 scars per cell. Out of 100 cells counted, 146 bud scars were counted. The wild yeast BY4735 had an average of 2.46 bud scars per cell (100 cells counted, 246 bud scars). This indicates that the knocked-out gene significantly slowed down the reproduction rate of these cells.
		+
		+	[[File:Yeast_2(2)_7_8]]
		+	''picture does not include all cells that were counted and added for a visual of bud scars''

@@ Line 48: / Line 48: @@
 ===Hydrogen Peroxide===
-[[Image:YER076C.png|center|thumb|600px|YER076C]]
+[[Image:YER076C.png|center|thumb|600px|The x-axis is time in minutes and the y-axis is OD600]]
 This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]].

← Older revision		Revision as of 21:30, 19 December 2022
Line 48:		Line 48:

	===Hydrogen Peroxide===		===Hydrogen Peroxide===
		+	[[Image:YER076C.png\|center\|thumb\|600px\|YER076C]]
	This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]].		This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]].

← Older revision		Revision as of 21:21, 19 December 2022
Line 48:		Line 48:

	===Hydrogen Peroxide===		===Hydrogen Peroxide===
		+	This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]].

	<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->		<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

@@ Line 47: / Line 47: @@
 The average doubling time for the YER076C (C4) gene in standard conditions was 87.5 minutes. Under caffeine stress, the average doubling time was 139.5 minutes. That is a 59.4% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.
+===Hydrogen Peroxide===
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

@@ Line 33: / Line 33: @@
 [[Image:Best Ethanol KO graph.png|Graph|center|700px|YJL133C-A]]
-YER076C had the most cell growth. The doubling time was 71.60
+YER076C had the most cell growth. The doubling time was 71.60. Ethanol didn't affect the growth to much.
 ===[[UW-Stout/Caffeine_FA22|Caffeine]]===