YDR307W - Revision history

Teagueb: /* Community Commentary */

2022-05-12T03:36:10Z

‎Community Commentary

Teagueb: /* Results */

2022-05-12T03:30:51Z

‎Results

Teagueb: /* Interpretation */

2022-05-12T02:48:54Z

‎Interpretation

Teagueb: /* Community Commentary */

2022-05-12T02:29:25Z

‎Community Commentary

Teagueb: /* UW Stout/Nystatin SP22 */

2022-05-09T04:52:14Z

‎UW Stout/Nystatin SP22

Teagueb: /* Community Commentary */

2022-05-09T03:58:41Z

‎Community Commentary

Teagueb at 17:40, 8 May 2022

2022-05-08T17:40:34Z

Teagueb at 00:29, 7 May 2022

2022-05-07T00:29:47Z

Teagueb at 18:55, 6 May 2022

2022-05-06T18:55:14Z

Teagueb at 18:41, 6 May 2022

2022-05-06T18:41:20Z

← Older revision		Revision as of 03:36, 12 May 2022
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	[[File:YDR-T2.jpg]]		[[File:YDR-T2.jpg]]
	The growth rates of the wild type yeast cells and the YDR307W strain are very similar. The YDR307W strain is not consistently different from the wild type yeast under Nystatin treatment, indicating that the removal of the YDR307W gene does not effect its resistance to Nystatin.		The growth rates of the wild type yeast cells and the YDR307W strain are very similar. The YDR307W strain is not consistently different from the wild type yeast under Nystatin treatment, indicating that the removal of the YDR307W gene does not effect its resistance to Nystatin.
		+
		+
		+	===[[UW-Stout/Formamide SP22]]===

@@ Line 180: / Line 180: @@
 ===Results===
-[[File:wildyeast.jpg|400px|]] [[File:trial222.jpg|400px|]] [[File:doublingtimestable.jpg|400px|]]
+[[File:wildyeast.jpg|400px|]] [[File:yeast.jpg|400px|]] [[File:doublingtimestable.jpg|400px|]]
@@ Line 186: / Line 186: @@
 *Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
 *Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)
 ===Interpretation===

← Older revision		Revision as of 02:48, 12 May 2022
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	===Interpretation===		===Interpretation===
		+	The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YDR307W strain. The YDR307W strain had doubling times significantly slower than the wild yeast cell, with a doubling time of 2156.61 min. It can be concluded that the transformed yeast cell, YDR307W, had a unique response to the formamide stress test used in the experiment.

	==References==		==References==

@@ Line 177: / Line 177: @@
 [[File:YDR-T2.jpg]]
 The growth rates of the wild type yeast cells and the YDR307W strain are very similar. The YDR307W strain is not consistently different from the wild type yeast under Nystatin treatment, indicating that the removal of the YDR307W gene does not effect its resistance to Nystatin.
 ==References==

@@ Line 176: / Line 176: @@
 [[File:YDR-T1.jpg]]
 [[File:YDR-T2.jpg]]
 ==References==

← Older revision		Revision as of 03:58, 9 May 2022
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	YDR307W displayed mild sensitivity to hydrogen peroxide within the growth curve, but it's inhibition range was slightly smaller than wild type cells treated with the same solution. This could be a difference in growth celling or that the knockout has caused a slightly lessened sensitivity to the stress factor.		YDR307W displayed mild sensitivity to hydrogen peroxide within the growth curve, but it's inhibition range was slightly smaller than wild type cells treated with the same solution. This could be a difference in growth celling or that the knockout has caused a slightly lessened sensitivity to the stress factor.
		+	===[[UW Stout/Nystatin SP22]]===
		+	As part of the University of Wisconsin Stout [[UW-Stout/About\|Orphan Gene Project]] this gene was tested by exposing the cells to 1µg/ml Nystatin solution.
		+	====Results====
		+	[[File:YDR-T1.jpg]]
		+	[[File:YDR-T2.jpg]]

	==References==		==References==

← Older revision		Revision as of 17:40, 8 May 2022
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	-According to the graph above, we can see that the KO yeast cell YDR307W follow a smaller sensitivity to the stress than the Wild type strain.		-According to the graph above, we can see that the KO yeast cell YDR307W follow a smaller sensitivity to the stress than the Wild type strain.
		+	-We can then conclude that the knocked-out gene in YDR307W doesn't have a significant impact on the growth of the yeast cell.
		+
		+
		+
		+
		+	'''[[UW-Stout/pH Acid SP22]]'''
		+
		+	[[File:Strain5.jpg\|350px\|]]
		+	Knocked out gene seems to have very little effect on cell growth in pH 7 environment based on the flattening of the growth curve. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve. Please see protocol for specific quantitative doubling time results.
		+

−	-~~We can then conclude that~~ the knocked-out gene ~~in YDR307W doesn't have~~ a ~~significant impact~~ on the growth of the ~~yeast cell~~.	+	[[File:Wild (1).jpg\|350px\|]]
		+	The wild-type strain was used as a control in this experiment; no genes were knocked out, it just represents how the experiment ran on the knocked-out gene strains affects a "typical" cell growth curve. Inclusion of this data on each knock-out strain growth curve graph made for difficult interpretation, so it has been separated. Please refer to this graph as a control when viewing the knock-out strain growth curve graph. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve.

← Older revision		Revision as of 00:29, 7 May 2022
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	'''[[UW-Stout/Nonionic Detergent SP22]]'''		'''[[UW-Stout/Nonionic Detergent SP22]]'''
		+
		+	As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 0.001% concentrated NLS. (Nonionic detergent)

	[[File:YDR307W.png\|500px]]		[[File:YDR307W.png\|500px]]

@@ Line 180: / Line 180: @@
 [[File:YDR307W.png|500px]]
 -According to the graph above, we can see that the KO yeast cell YDR307W follow a smaller sensitivity to the stress than the Wild type strain.
 -We can then conclude that the knocked-out gene in YDR307W doesn't have a significant impact on the growth of the yeast cell.

← Older revision		Revision as of 18:41, 6 May 2022
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	[[File:YDR307W.png\|500px]]		[[File:YDR307W.png\|500px]]
		+
		+	-According to the graph above, we can see that the KO yeast cell YDR307W follow a smaller sensitivity to the stress than the Wild type strain.
		+
		+	-We can then conclude that the knocked-out gene in YDR307W doesn't have a significant impact on the growth of the yeast cell.