YBR284W - Revision history

Teagueb: /* UW-Stout/Copper FA21 */

2021-12-21T22:25:30Z

‎UW-Stout/Copper FA21

2021-12-21T22:03:42Z

‎UW-Stout/Copper FA21

2021-12-21T21:37:55Z

‎UW-Stout/Copper FA21

2021-12-21T21:35:33Z

‎UW-Stout/Copper FA21

2021-12-21T21:34:34Z

‎UW-Stout/Copper FA21

2021-12-21T21:33:26Z

2021-12-20T23:31:24Z

2021-12-20T23:30:08Z

2021-12-20T22:48:51Z

‎Community Commentary

2021-12-14T21:58:34Z

‎pH

← Older revision		Revision as of 22:25, 21 December 2021
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	[[File:ybr2.jpg]]		[[File:ybr2.jpg]]

−	The average doubling time for YBR284W stressed with our CuSO4 solution was ~~53.9~~, while unstressed was ~~41.34~~. The stressed version grew approximately 23% slower than the unstressed version. The unstressed version appears to have also grown larger overall.	+	The average doubling time for YBR284W stressed with our CuSO4 solution was 239 minutes, while unstressed was 207. The stressed version grew approximately 14% slower than the unstressed version. The unstressed version appears to have also grown larger overall.

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 ===[[UW-Stout/Copper FA21]]===
-[[File:ybr284w.jpg]]
+[[File:ybr2.jpg]]
 The average doubling time for YBR284W stressed with our CuSO4 solution was 53.9, while unstressed was 41.34. The stressed version grew approximately 23% slower than the unstressed version. The unstressed version appears to have also grown larger overall.

← Older revision		Revision as of 21:37, 21 December 2021
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	[[File:ybr284w.jpg]]		[[File:ybr284w.jpg]]
		+
		+	The average doubling time for YBR284W stressed with our CuSO4 solution was 53.9, while unstressed was 41.34. The stressed version grew approximately 23% slower than the unstressed version. The unstressed version appears to have also grown larger overall.

@@ Line 62: / Line 62: @@
 The YBR284W yeast strain doubling time without any stress was 174 minutes which is pretty similar to the wild type with 182 minutes. When both strains were stressed by G418 they YBR284W doubling time was 173 minutes and the wild type was 178 minutes. (These times are averaged between three trials)
 Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/G418_FA21]
 ===[[UW-Stout/Ethanol FA21]]===
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 * The Yeast Strain of YBR284W was not affected in any waty by the 18.5 % Ethanol it still has a steady growth curve
 [[UW-Stout/Ethanol FA21]]

← Older revision		Revision as of 23:30, 20 December 2021
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	Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/G418_FA21]		Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/G418_FA21]

		+	===[[UW-Stout/Ethanol FA21]]===
		+	[[File:Yeast ToutureV2.jpg\|400px]]
		+	* The Yeast Strain of YBR284W was not affected in any waty by the 18.5 % Ethanol it still has a steady growth curve
		+	[[UW-Stout/Ethanol FA21]]

← Older revision		Revision as of 22:48, 20 December 2021
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	Cells grown with this gene (YBR284W) knocked out had an average of 1.18 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 114 YBR284W cells counted, 135 bud scars were seen. This indicated that the YBR284W gene slows reproduction rate and thus bud scarring in yeast cells.		Cells grown with this gene (YBR284W) knocked out had an average of 1.18 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 114 YBR284W cells counted, 135 bud scars were seen. This indicated that the YBR284W gene slows reproduction rate and thus bud scarring in yeast cells.
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		+	===[[UW-Stout/G418 FA21]]===
		+
		+	[[File:YBR284W Vs BY4735 G418.png\|left\|thumb\|400px]]
		+
		+
		+	The YBR284W yeast strain doubling time without any stress was 174 minutes which is pretty similar to the wild type with 182 minutes. When both strains were stressed by G418 they YBR284W doubling time was 173 minutes and the wild type was 178 minutes. (These times are averaged between three trials)
		+	Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/G418_FA21]
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	<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->		<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

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 YBR284W
 *When this strain was exposed to a citric acid buffer with a pH of 2.6, there did not appear to be a significant change in the growth rate of the strain
-  ==protocol==
+  Protocol:
   [[https://wiki.yeastgenome.org/index.php/UW-Stout/pH_FA21]]