YBR220C - Revision history

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YBR220C */

2019-12-18T17:29:44Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YBR220C

Teagueb at 08:51, 18 December 2019

2019-12-18T08:51:44Z

Teagueb at 08:06, 18 December 2019

2019-12-18T08:06:18Z

Teagueb at 06:20, 18 December 2019

2019-12-18T06:20:50Z

Teagueb at 05:50, 18 December 2019

2019-12-18T05:50:23Z

Teagueb: /* Growth in Proline and Ammonium Sulfate */

2019-12-18T02:43:07Z

‎Growth in Proline and Ammonium Sulfate

Teagueb: /* KO 6 Growth in Proline and Ammonium Sulfate */

2019-12-18T02:28:09Z

‎KO 6 Growth in Proline and Ammonium Sulfate

Teagueb: /* KO 6 Growth in Proline and Ammonium Sulfate */

2019-12-18T01:47:06Z

‎KO 6 Growth in Proline and Ammonium Sulfate

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YBR220C */

2019-12-17T23:13:31Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YBR220C

Teagueb: /* Community Commentary */

2019-12-17T23:12:27Z

‎Community Commentary

@@ Line 86: / Line 86: @@
 [[File:JaredJ7.PNG]]
 [[File:JJ7.PNG]]
 ==Ethanol Tolerance in YBR220C==

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 == [https://wiki.yeastgenome.org/index.php?title=UW-Stout/Hydroxyurea_FA19&oldid=401696 Hydroxyurea] ==
 [[File:AvaYBR220C.png]]
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

@@ Line 100: / Line 100: @@
 The Wild Type BY4735 has data located at the Nitrogen Starvation Protocol.
 ==[[UW-Stout/Nitrogen_starvation_FA19|Nitrogen starvation]]==
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

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 The graph above shows how dilutions P2 (30 µg/ml) and E2 (10% ethanol solution) impacted the growth rate of the knockout yeast strain YBR220C over 24 hours. The concentration did not double for test 3 of P2 for this experiment, so by taking only the first two test results, the doubling rate averages to around 1,063 minutes for only ethanol. As for the Prednisone with ethanol, the doubling rate averages to around 923 minutes. Overall, the Prednisone increased the rate of growth by almost 140 minutes. These results conclude that Prednisone slightly promotes the growth of yeast cells without the YBR220C gene.
 [[File:Nitrogen_Starvation_YBR220C.jpg|463 x 599|Caption]]
 ==[[UW-Stout/Nitrogen_starvation_FA19|Nitrogen starvation]]==

← Older revision		Revision as of 05:50, 18 December 2019
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	The graph above shows how dilutions P2 (30 µg/ml) and E2 (10% ethanol solution) impacted the growth rate of the knockout yeast strain YBR220C over 24 hours. The concentration did not double for test 3 of P2 for this experiment, so by taking only the first two test results, the doubling rate averages to around 1,063 minutes for only ethanol. As for the Prednisone with ethanol, the doubling rate averages to around 923 minutes. Overall, the Prednisone increased the rate of growth by almost 140 minutes. These results conclude that Prednisone slightly promotes the growth of yeast cells without the YBR220C gene.		The graph above shows how dilutions P2 (30 µg/ml) and E2 (10% ethanol solution) impacted the growth rate of the knockout yeast strain YBR220C over 24 hours. The concentration did not double for test 3 of P2 for this experiment, so by taking only the first two test results, the doubling rate averages to around 1,063 minutes for only ethanol. As for the Prednisone with ethanol, the doubling rate averages to around 923 minutes. Overall, the Prednisone increased the rate of growth by almost 140 minutes. These results conclude that Prednisone slightly promotes the growth of yeast cells without the YBR220C gene.
		+	[[File:Nitrogen_Starvation_YBR220C.jpg\|463 x 599\|Caption]]
		+	==[[UW-Stout/Nitrogen_starvation_FA19\|Nitrogen starvation]]==

	<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->		<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

← Older revision		Revision as of 02:43, 18 December 2019
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−	===Growth in Proline and Ammonium Sulfate===	+	===[[UW Stout/Proline FA19\|Growth in Proline and Ammonium Sulfate]]===

← Older revision		Revision as of 02:28, 18 December 2019
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−	===~~KO 6~~ Growth in Proline and Ammonium Sulfate===	+	===Growth in Proline and Ammonium Sulfate===

← Older revision		Revision as of 01:47, 18 December 2019
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	[[File:KO_6.png\|650 px]]		[[File:KO_6.png\|650 px]]

		+
		+	For YBR220C, when compared to growth in proline media and ammonium sulfate media, this strand grew faster in ammonium sulfate with an average doubling of 37.67 minutes. The average doubling time in the proline media was 41.87. In the wild type strands though, the yeast grew faster in proline media with an average doubling time of 42.2 minutes compared to 50.2 minutes when grown in ammonium sulfate.

	===1% Formamide Yeast Torture[[https://wiki.yeastgenome.org/index.php/UW_Stout/Formamide_FA19]]===		===1% Formamide Yeast Torture[[https://wiki.yeastgenome.org/index.php/UW_Stout/Formamide_FA19]]===

← Older revision		Revision as of 23:13, 17 December 2019
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	===Zone of Inhibition of 30% [https://wiki.yeastgenome.org/index.php/UW-Stout/Hydrogen_peroxide_FA19 Hydrogen Peroxide] on YBR220C===		===Zone of Inhibition of 30% [https://wiki.yeastgenome.org/index.php/UW-Stout/Hydrogen_peroxide_FA19 Hydrogen Peroxide] on YBR220C===
		+	[[File:JaredJ7.PNG]]
		+	[[File:JJ7.PNG]]

	==Ethanol Tolerance in YBR220C==		==Ethanol Tolerance in YBR220C==

← Older revision		Revision as of 23:12, 17 December 2019
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	This graph shows the growth curve of the knockout yeast strain YBR220C and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YBR220C with 1% formamide was 12,783.54 minutes, while the unstressed strain was 3,360.93 minutes. This concluded that the stressed out strain is very sensitive to 1% formamide, and grows at a much slower pace in this environment. Our stressed wild types doubling time was 2,865.53 minutes, and the unstressed was 2,543.42 minutes. The stressed wild type in this experiment proved to show a slight more sensitivity than the unstressed, meaning that addition of 1% formamide for this strain slows down growth.		This graph shows the growth curve of the knockout yeast strain YBR220C and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YBR220C with 1% formamide was 12,783.54 minutes, while the unstressed strain was 3,360.93 minutes. This concluded that the stressed out strain is very sensitive to 1% formamide, and grows at a much slower pace in this environment. Our stressed wild types doubling time was 2,865.53 minutes, and the unstressed was 2,543.42 minutes. The stressed wild type in this experiment proved to show a slight more sensitivity than the unstressed, meaning that addition of 1% formamide for this strain slows down growth.
		+
		+	===Zone of Inhibition of 30% [https://wiki.yeastgenome.org/index.php/UW-Stout/Hydrogen_peroxide_FA19 Hydrogen Peroxide] on YBR220C===

	==Ethanol Tolerance in YBR220C==		==Ethanol Tolerance in YBR220C==