YBR138C - Revision history

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YBR138C */

2019-12-18T17:14:19Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YBR138C

Teagueb at 08:51, 18 December 2019

2019-12-18T08:51:19Z

Teagueb at 08:02, 18 December 2019

2019-12-18T08:02:35Z

Teagueb at 06:20, 18 December 2019

2019-12-18T06:20:35Z

Teagueb at 04:56, 18 December 2019

2019-12-18T04:56:59Z

Teagueb at 04:56, 18 December 2019

2019-12-18T04:56:21Z

Teagueb: /* Community Commentary */

2019-12-18T02:52:43Z

‎Community Commentary

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YKR005C */

2019-12-17T21:35:41Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YKR005C

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YKR005C */

2019-12-17T21:35:24Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YKR005C

Teagueb at 21:29, 17 December 2019

2019-12-17T21:29:50Z

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 [[File:JJ6.PNG]]
 [[File:JaredJ6.PNG]]
 <protect>

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 == [https://wiki.yeastgenome.org/index.php?title=UW-Stout/Hydroxyurea_FA19&oldid=401696 Hydroxyurea] ==
 [[File:AvaYBR138C.png]]
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

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 The Wild Type BY4735 has data located at the Nitrogen Starvation Protocol.
 ==[[UW-Stout/Nitrogen_starvation_FA19|Nitrogen starvation]]==
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

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 This graph shows the growth curve of the knockout yeast strain YBR138C and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YBR138C with 1% formamide was 2,710.55 minutes, while the unstressed strain was 3,131.33 minutes. This concluded that the unstressed strain is slightly sensitive to 1% formamide, and grows at a slower pace in this environment. Our stressed wild types doubling time was 3,459.91 minutes, and the unstressed was 2,468.04 minutes. This shows that the addition of 1% formamide constrains growth for the stressed wild type.
 [[File:Nitrogen_Starvation_YBR138C.jpg|463 x 599|Caption]]
 ==[[UW-Stout/Nitrogen_starvation_FA19|Nitrogen starvation]]==

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 This graph shows the growth curve of the knockout yeast strain YBR138C and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YBR138C with 1% formamide was 2,710.55 minutes, while the unstressed strain was 3,131.33 minutes. This concluded that the unstressed strain is slightly sensitive to 1% formamide, and grows at a slower pace in this environment. Our stressed wild types doubling time was 3,459.91 minutes, and the unstressed was 2,468.04 minutes. This shows that the addition of 1% formamide constrains growth for the stressed wild type.
-[[File:Nitrogen_Starvation_YBR128C.jpg|463 x 599|Caption]]
+[[File:Nitrogen_Starvation_YBR138C.jpg|463 x 599|Caption]]
 ==[[UW-Stout/Nitrogen_starvation_FA19|Nitrogen starvation]]==

← Older revision		Revision as of 02:52, 18 December 2019
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	The graph above shows the effect of 3% DMSO on YBR138C. From the data represented the average doubling rate for the YBR138C knockout strain without DMSO was 570 minutes. When DMSO is added the average doubling time increased to 835 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YBR138C with DMSO we can see that without the YBR138C gene the yeast was able to grow more quickly under DMSO induced stress, so much so that it almost halves the doubling time. Given both controls it also appears that the YBR138C gene has very little effect on the growth of the yeast in optimal conditions.		The graph above shows the effect of 3% DMSO on YBR138C. From the data represented the average doubling rate for the YBR138C knockout strain without DMSO was 570 minutes. When DMSO is added the average doubling time increased to 835 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YBR138C with DMSO we can see that without the YBR138C gene the yeast was able to grow more quickly under DMSO induced stress, so much so that it almost halves the doubling time. Given both controls it also appears that the YBR138C gene has very little effect on the growth of the yeast in optimal conditions.
		+
		+
		+
		+	===[[UW Stout/Proline FA19\|Growth in Proline and Ammonium Sulfate]]===
		+	[[File:KO5.png\|650 px]]
		+
		+	This strand had an average doubling rate of 41.43 minutes in proline media and 42.23 minutes in ammonium sulfate media. There is not much of a difference, but it grew faster in proline media. For the wild types, the fastest average doubling rate was in ammonium sulfate media at 47.3 minutes. The average doubling time in proline was 50.63 minutes.

	==Ethanol Tolerance in YBR138C==		==Ethanol Tolerance in YBR138C==

← Older revision		Revision as of 21:29, 17 December 2019
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		+	===Zone of Inhibition of 30% [https://wiki.yeastgenome.org/index.php/UW-Stout/Hydrogen_peroxide_FA19 Hydrogen Peroxide] on YKR005C===