YBR033W - Revision history

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YBR033W */

2019-12-18T16:47:53Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YBR033W

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YBR033W */

2019-12-18T16:47:34Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YBR033W

Teagueb at 08:50, 18 December 2019

2019-12-18T08:50:52Z

Teagueb at 07:45, 18 December 2019

2019-12-18T07:45:50Z

Teagueb at 06:20, 18 December 2019

2019-12-18T06:20:04Z

Teagueb at 04:51, 18 December 2019

2019-12-18T04:51:25Z

Teagueb: /* Growth in Proline and Ammonium Sulfate */

2019-12-18T02:41:13Z

‎Growth in Proline and Ammonium Sulfate

Teagueb: /* Community Commentary */

2019-12-18T02:34:12Z

‎Community Commentary

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YKR005C */

2019-12-17T21:36:52Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YKR005C

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YKR005C */

2019-12-17T21:29:28Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YKR005C

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 [[File:JJ5.PNG]]
 [[File:JaredJ5.PNG]]
 The bar graph represents the zone of inhibition of the knockout strain versus the wild type. Our data collected shows that the knockout strain saw a larger zone of inhibition (cells were not able to grow there). The error bar on the knockout strain represents the standard deviation of the experiment and how recreatable our results were with these results. Using these observations I can say that the yeast strain YBR033W undergoes more stress from the oxidative properties of 30 percent hydrogen peroxide. The zone of inhibition is larger because the yeast cells are not able to grow as well as the wild type can in these conditions. For the strain YBR033W our plate values were as follows (in cm) 2.4, 2.3 and 2.4 compared to our wild type plate which stayed constant 2.1, 2.1 and 2.2 cm
 <protect>

@@ Line 113: / Line 113: @@
 [[File:JJ5.PNG]]
 [[File:JaredJ5.PNG]]
+The bar graph represents the zone of inhibition of the knockout strain versus the wild type. Our data collected shows that the knockout strain saw a larger zone of inhibition (cells were not able to grow there). The error bar on the knockout strain represents the standard deviation of the experiment and how recreatable our results were with these results. Using these observations I can say that the yeast strain YBR033W undergoes more stress from the oxidative properties of 30 percent hydrogen peroxide. The zone of inhibition is larger because the yeast cells are not able to grow as well as the wild type can in these conditions. For the strain YBR033W our plate values were as follows (in cm) 2.4, 2.3 and 2.4 compared to our wild type plate which stayed constant 2.1, 2.1 and 2.2 cm
 <protect>

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 == [https://wiki.yeastgenome.org/index.php?title=UW-Stout/Hydroxyurea_FA19&oldid=401696 Hydroxyurea] ==
 [[File:AvaYBR033W.png]]
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

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+== [https://wiki.yeastgenome.org/index.php?title=UW-Stout/Hydroxyurea_FA19&oldid=401696 Hydroxyurea] ==
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

← Older revision		Revision as of 06:20, 18 December 2019
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	[[File:Nitrogen_Starvation_YBR033W.jpg\|463 x 599\|Caption]]		[[File:Nitrogen_Starvation_YBR033W.jpg\|463 x 599\|Caption]]
		+
		+	The Wild Type BY4735 has data located at the Nitrogen Starvation Protocol.
	==[[UW-Stout/Nitrogen_starvation_FA19\|Nitrogen starvation]]==		==[[UW-Stout/Nitrogen_starvation_FA19\|Nitrogen starvation]]==

← Older revision		Revision as of 04:51, 18 December 2019
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	This graph shows the growth curve of the knockout yeast strain YBR033W and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YBR033W with 1% formamide was 3,771.50 minutes, while the unstressed strain was 3,181.55 minutes. This concluded that the stressed out strain is slightly sensitive to 1% formamide, and grows as a slower pace in this environment. Our stressed wild types doubling time was 3,459.91 minutes, and the unstressed was 2,468.04 minutes. Again, proving that the addition of 1% formamide constrains growth.		This graph shows the growth curve of the knockout yeast strain YBR033W and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YBR033W with 1% formamide was 3,771.50 minutes, while the unstressed strain was 3,181.55 minutes. This concluded that the stressed out strain is slightly sensitive to 1% formamide, and grows as a slower pace in this environment. Our stressed wild types doubling time was 3,459.91 minutes, and the unstressed was 2,468.04 minutes. Again, proving that the addition of 1% formamide constrains growth.

		+	[[File:Nitrogen_Starvation_YBR033W.jpg\|463 x 599\|Caption]]
		+	==[[UW-Stout/Nitrogen_starvation_FA19\|Nitrogen starvation]]==

	<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->		<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

← Older revision		Revision as of 02:41, 18 December 2019
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−	===Growth in Proline and Ammonium Sulfate===	+	===[[UW Stout/Proline FA19\|Growth in Proline and Ammonium Sulfate]]===

	[[File:KO4.png\|650 px]]		[[File:KO4.png\|650 px]]

← Older revision		Revision as of 02:34, 18 December 2019
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	The graph above shows the effect of 3% DMSO on YBR033W. From the data represented the average doubling rate for the YBR033W knockout strain without DMSO was 634 minutes. When DMSO is added the average doubling time increased to 1292 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YBR033W with DMSO we can see that without the YBR033W gene the yeast was able to grow more quickly under DMSO induced stress.		The graph above shows the effect of 3% DMSO on YBR033W. From the data represented the average doubling rate for the YBR033W knockout strain without DMSO was 634 minutes. When DMSO is added the average doubling time increased to 1292 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YBR033W with DMSO we can see that without the YBR033W gene the yeast was able to grow more quickly under DMSO induced stress.
		+
		+
		+
		+	===Growth in Proline and Ammonium Sulfate===
		+
		+	[[File:KO4.png\|650 px]]
		+
		+	The YBR033W strand had similar average doubling rates in both medias. In proline media, the average doubling rate was 35.97 minutes, while it was 36.5 minutes. The wild type also had similar average growth rates also. They were 49.33 and 49.27 minutes in proline and ammonium sulfate media respectively.

	==Ethanol Tolerance in YBR033W==		==Ethanol Tolerance in YBR033W==

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-===Zone of Inhibition of 30% [https://wiki.yeastgenome.org/index.php/UW-Stout/Hydrogen_peroxide_FA19 Hydrogen Peroxide] on YKR005C===
+===Zone of Inhibition of 30% [https://wiki.yeastgenome.org/index.php/UW-Stout/Hydrogen_peroxide_FA19 Hydrogen Peroxide] on YBR033W===
 [[File:JJ5.PNG]]