YBL081W - Revision history

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YBL081W */

2019-12-18T16:43:58Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YBL081W

Teagueb at 08:50, 18 December 2019

2019-12-18T08:50:27Z

Teagueb at 07:26, 18 December 2019

2019-12-18T07:26:11Z

Teagueb at 06:19, 18 December 2019

2019-12-18T06:19:51Z

Teagueb at 04:40, 18 December 2019

2019-12-18T04:40:27Z

Teagueb: /* Growth in Proline and Ammonium Sulfate */

2019-12-18T02:53:08Z

‎Growth in Proline and Ammonium Sulfate

Teagueb: /* Growth in Proline and Ammonium Sulfate */

2019-12-18T02:52:57Z

‎Growth in Proline and Ammonium Sulfate

Teagueb: /* Growth in Proline and Ammonium Sulfate */

2019-12-18T02:39:41Z

‎Growth in Proline and Ammonium Sulfate

Teagueb: /* Community Commentary */

2019-12-18T02:20:45Z

‎Community Commentary

Teagueb: /* Zone of Inhibition of 30% Hydrogen Peroxide on YKR005C */

2019-12-17T21:36:22Z

‎Zone of Inhibition of 30% Hydrogen Peroxide on YKR005C

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 [[File:JJ3.PNG]]
 [[File:JaredJ3.PNG]]
 <protect>

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 ==[https://wiki.yeastgenome.org/index.php?title=UW-Stout/Hydroxyurea_FA19&oldid=401696 Hydroxyurea]==
 [[File:AvaYBL081W.png]]
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

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 The Wild Type BY4735 has data located at the Nitrogen Starvation Protocol.
 ==[[UW-Stout/Nitrogen_starvation_FA19|Nitrogen starvation]]==
 <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

← Older revision		Revision as of 06:19, 18 December 2019
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	[[File:Nitrogen_Starvation_YBL081W.jpg\|463 x 599\|Caption]]		[[File:Nitrogen_Starvation_YBL081W.jpg\|463 x 599\|Caption]]
		+
		+	The Wild Type BY4735 has data located at the Nitrogen Starvation Protocol.
	==[[UW-Stout/Nitrogen_starvation_FA19\|Nitrogen starvation]]==		==[[UW-Stout/Nitrogen_starvation_FA19\|Nitrogen starvation]]==

← Older revision		Revision as of 04:40, 18 December 2019
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	The graph above shows how dilutions P2 (30 µg/ml) and E2 (10% ethanol solution) impacted the growth rate of the knockout yeast strain YBL081W over 24 hours. The concentration did not double for test 3 of P2 for this experiment so by taking only the first two test results, the doubling rate averages to around 1,120 minutes for only ethanol. As for the Prednisone with ethanol, the doubling rate averages to around 938 minutes. Overall, the Prednisone increased the rate of growth by almost 182 minutes. These results conclude that Prednisone promotes the growth of yeast cells without the YBL081W gene.		The graph above shows how dilutions P2 (30 µg/ml) and E2 (10% ethanol solution) impacted the growth rate of the knockout yeast strain YBL081W over 24 hours. The concentration did not double for test 3 of P2 for this experiment so by taking only the first two test results, the doubling rate averages to around 1,120 minutes for only ethanol. As for the Prednisone with ethanol, the doubling rate averages to around 938 minutes. Overall, the Prednisone increased the rate of growth by almost 182 minutes. These results conclude that Prednisone promotes the growth of yeast cells without the YBL081W gene.
		+
		+	[[File:Nitrogen_Starvation_YBL081W.jpg\|463 x 599\|Caption]]
		+	==[[UW-Stout/Nitrogen_starvation_FA19\|Nitrogen starvation]]==

	<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->		<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

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 ===[[UW Stout/Proline FA19|Growth in Proline and Ammonium Sulfate]]===
 [[File:KO3.png|650 px]]
 This strand of yeast grew the fastest in ammonium sulfate media at 36.57 minutes as the average doubling rate. The average doubling rate in proline was 40.67 minutes, a whole 4.1 minutes slower than the average doubling rate in ammonium sulfate. The wild type also grew faster in ammonium sulfate with an average doubling rate of 46.1 minutes. In proline the average doubling rate of the wild type was 49 minutes.

← Older revision		Revision as of 02:39, 18 December 2019
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−	===Growth in Proline and Ammonium Sulfate===	+	===[[UW Stout/Proline FA19\|Growth in Proline and Ammonium Sulfate]]===
	[[File:KO3.png\|650 px]]		[[File:KO3.png\|650 px]]

← Older revision		Revision as of 02:20, 18 December 2019
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	This graph shows the growth curve of the knockout yeast strain YBL081W and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YBL081W with 1% formamide was 2,003.40 minutes, while the unstressed strain was 3,230.61 minutes. This concluded that the stressed out strain is not sensitive to 1% formamide, and grows at a faster pace in this environment. Our stressed wild types doubling time was 2,799.18 minutes, and the unstressed was 1,823.83 minutes. Proving that the addition of 1% formamide makes this strain more sensitive to it's surroundings and leads to slower growth.		This graph shows the growth curve of the knockout yeast strain YBL081W and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YBL081W with 1% formamide was 2,003.40 minutes, while the unstressed strain was 3,230.61 minutes. This concluded that the stressed out strain is not sensitive to 1% formamide, and grows at a faster pace in this environment. Our stressed wild types doubling time was 2,799.18 minutes, and the unstressed was 1,823.83 minutes. Proving that the addition of 1% formamide makes this strain more sensitive to it's surroundings and leads to slower growth.
		+
		+
		+
		+	===Growth in Proline and Ammonium Sulfate===
		+	[[File:KO3.png\|650 px]]

	==Ethanol Tolerance in YBL081W==		==Ethanol Tolerance in YBL081W==

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 J Biol Chem 278(5):3265-74</ref>
 -->
-===Zone of Inhibition of 30% [https://wiki.yeastgenome.org/index.php/UW-Stout/Hydrogen_peroxide_FA19 Hydrogen Peroxide] on YKR005C===
+===Zone of Inhibition of 30% [https://wiki.yeastgenome.org/index.php/UW-Stout/Hydrogen_peroxide_FA19 Hydrogen Peroxide] on YBL081W===
 [[File:JJ3.PNG]]
 [[File:JaredJ3.PNG]]