UW Stout/Sucrose Fermentation SP22 - Revision history

Teagueb: /* Results of knockout experiment */

2022-05-04T16:48:36Z

‎Results of knockout experiment

2022-05-03T20:09:07Z

‎Results of knockout experiment

2022-05-03T20:08:21Z

2022-05-03T19:44:47Z

2022-05-03T19:33:34Z

‎Make Fermentation Solution

2022-05-03T19:28:54Z

2022-05-03T19:20:52Z

2022-05-03T19:09:43Z

2022-05-03T19:07:43Z

‎Make Fermentation Solution

2022-05-03T19:05:26Z

‎Make Fermentation Solution

@@ Line 151: / Line 151: @@
 |-
 |YCL049C
+|0.4078
+|0.3052
+|0.1969
 |-
 |YGR079W
+|0.4042
+|0.1589
+|0.0080
 |-
 |YBL113C

@@ Line 105: / Line 105: @@
 # Set up HPLC machine and run using standards that test for ethanol, glucose, and fructose.
-====Results of knockout experiment====
+==Results of knockout experiment==
 All measurements are based off of 500ul of solution
 ===Table 2===
 {| border="1" cellspacing="4" cellpadding="2"

@@ Line 85: / Line 85: @@
 We found through qualitative analysis that the 4% total sucrose in solution produced the most CO2 after 48 hours.
-==Experimental Procedure==
+==Knockout Procedure==
 ====Set up yeast====
@@ Line 104: / Line 104: @@
 # add 500ul of supernatant and 500ul of DI water to HPLC test tubes
 # Set up HPLC machine and run using standards that test for ethanol, glucose, and fructose.

← Older revision		Revision as of 19:44, 3 May 2022
Line 98:		Line 98:
	# Vortex solution for 30 seconds		# Vortex solution for 30 seconds
	# Store in 30°C incubator for 48 hours		# Store in 30°C incubator for 48 hours
		+
		+	====Prepare for HPLC====
		+	# Take 1ml aliquot from fermentation
		+	# Centrifuge for 2 minutes at 15000x gravity
		+	# add 500ul of supernatant and 500ul of DI water to HPLC test tubes
		+	# Set up HPLC machine and run using standards that test for ethanol, glucose, and fructose.

@@ Line 97: / Line 97: @@
 # Follow solution 1 in table 1 for each gene
 # Vortex solution for 30 seconds
-# Store in 30°C incubator
+# Store in 30°C incubator for 48 hours

@@ Line 19: / Line 19: @@
 * Centrifuge
 * Vortex
 ==Pilot Experiment Procedure==
@@ Line 28: / Line 27: @@
 # Remove supernatant
 # Add 1 ml of PBS buffer to yeast pellet
-# Vortex
+# Vortex for 30 seconds
 ====Make Fermentation Solution====
-# Mix the following ingredients for each concentration *See table 1
+# Mix the following ingredients for each solution *See table 1
 # Vortex solution for 30 seconds
 # Store in 30°C incubator
@@ Line 40: / Line 39: @@
 {| border="1" cellspacing="4" cellpadding="2"
+|
-|Concentration 1
+|Solution 1
-|Concentration 2
+|Solution 2
-|Concentration 3
+|Solution 3
-|Concentration 4
+|Solution 4
 |-
 |Yeast
@@ Line 84: / Line 83: @@
 ===Results===
-We found through qualitative analysis that the 4% total sucrose in concentration produced the most CO2 after 48 hours.
+We found through qualitative analysis that the 4% total sucrose in solution produced the most CO2 after 48 hours.
 ==Experimental Procedure==
@@ Line 93: / Line 92: @@
 # Remove supernatant
 # Add 1 ml of PBS buffer to yeast pellet
-# Vortex
+# Vortex for 30 seconds

@@ Line 11: / Line 11: @@
 ** 0.85g YNB, 2.5 Ammonium Sulfate, 0.32g CSM, 5ml 100x Leucine, 100x Histine, 100x Uracil, 435 ml DI H2O
 * 40% Sucrose Solution
 ==Pilot Experiment Procedure==
 ====Set up yeast====
-# Collect 1 ml of yeast solution
+# Collect 1 ml of yeast solution (approximately 1 million cells)
 # Centrifuge for 3 minutes at 1000x gravity
 # Remove supernatant
 # Add 1 ml of PBS buffer to yeast pellet
 ====Make Fermentation Solution====
@@ Line 74: / Line 84: @@
 ===Results===
-We found that the 4% total sucrose in concentration produced the most CO2 after 48 hours.
+We found through qualitative analysis that the 4% total sucrose in concentration produced the most CO2 after 48 hours.

← Older revision		Revision as of 19:09, 3 May 2022
Line 71:		Line 71:
	\|0.5%		\|0.5%
	\|}		\|}
		+
		+	===Results===
		+
		+	We found that the 4% total sucrose in concentration produced the most CO2 after 48 hours.

@@ Line 68: / Line 68: @@
 # Vortex solution for 30 seconds
-# Store in 30C incubator
+# Store in 30°C incubator
 # Sample solution (for ethanol production) in 24 and 48 hours.