UW-Stout/Nonionic Detergent SP22 - Revision history

Teagueb at 20:29, 5 May 2022

2022-05-05T20:29:02Z

2022-05-05T20:28:19Z

2022-05-05T20:27:53Z

2022-05-05T18:31:35Z

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@@ Line 69: / Line 69: @@
 Temperature: 30°C
 Mode: Kinetic
 Wavelength: 600 nm
 Interval: 5 minutes
 Total run time: 24 hours
 Shake before read: 30 seconds
@@ Line 186: / Line 191: @@
 Temperature: 30°C
 Mode: Kinetic
 Wavelength: 600 nm
 Interval: 5 minutes
 Total run time: 24 hours
 Shake before read: 30 seconds

@@ Line 18: / Line 18: @@
 .	Make a 0.1% solution of NLS by putting 1µl of a 10% solution of NLS in 99 mL of water.
 This is going to give you a 100 mL solution with a 0.1 concentration of NLS.
 .	Use the 0.1% solution of NLS to make 7 different concentrations of N-Lauroylsarcosine (0%, 0.001%, 0.002%, 0.005%, 0.01%, 0.02%, 0.05%). To increase solubility, temperature can be increased to 25-30°C.
 .	Set up 8 wells according to following table.

@@ Line 128: / Line 128: @@
 '''4. Procedure:'''
 .	Make a 0,1% solution of NLS by putting 1µl of a 10% NLS solution in 99µl of DI water.
 .	Mix the 0,1% solution with enough water to dilute the concentration to 0.001% while having a 50µl solution. Then, mix it with 9 different KO yeast cell to make nine different 100µl solution.
 .	Set up 9 wells according to following table. Do two experiments of this.

← Older revision		Revision as of 18:31, 5 May 2022
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	[[File:Growth rate of KO yeast cell at 0.001% NLS (Logarithmic).png\|500px]]		[[File:Growth rate of KO yeast cell at 0.001% NLS (Logarithmic).png\|500px]]
		+
		+	'''Doubling time of Well 1-9:'''
		+
		+	Td (Well 1): 580 minutes
		+	Td (Well 2): 481 minutes
		+	Td (Well 3): 412 minutes
		+	Td (Well 4): 448.1 minutes
		+	Td (Well 5): 537.8 minutes
		+	Td (Well 6): 570.6 minutes
		+	Td (Well 7): 423 minutes
		+	Td (Well 8): 1766 minutes
		+	Td (Well 9): 1209 minutes

← Older revision		Revision as of 18:30, 5 May 2022
Line 196:		Line 196:

	-Under is a graph with a Y axis scale set to logarithmic so we can find the doubling-time of the KO yeast cell when mixed with a 0.001% solution of NLS:		-Under is a graph with a Y axis scale set to logarithmic so we can find the doubling-time of the KO yeast cell when mixed with a 0.001% solution of NLS:
		+
		+	[[File:Growth rate of KO yeast cell at 0.001% NLS (Logarithmic).png\|500px]]

@@ Line 191: / Line 191: @@
 . Collect the data and create a graph to compare results.
--Here is the graph obtained after following the procedure above and putting it in the plate reader for 24 hours:
+-Here is the graph for experiment 1 obtained after following the procedure above and putting it in the plate reader for 24 hours:
 [[File:Growth rate of different KO yeast cell when mixed with a 0.001% concentration solution of NLS.png|500px]]

← Older revision		Revision as of 18:26, 5 May 2022
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	-Here is the graph obtained after following the procedure above and putting it in the plate reader for 24 hours:		-Here is the graph obtained after following the procedure above and putting it in the plate reader for 24 hours:
		+
		+	[[File:Growth rate of different KO yeast cell when mixed with a 0.001% concentration solution of NLS.png\|500px]]

← Older revision		Revision as of 18:24, 5 May 2022
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	6. Collect the data and create a graph to compare results.		6. Collect the data and create a graph to compare results.
		+
		+	-Here is the graph obtained after following the procedure above and putting it in the plate reader for 24 hours:

@@ Line 2: / Line 2: @@
 == '''Pilot experiment:''' ==
-  Testing NLS effect on WT yeast cells growth
+  NLS effect on WT yeast cells growth
 . '''Introduction:''' In this experiment, we will be testing the effect of N-Lauroylsarcosine on the speed of growth of wild type yeast cells. Our objective is to find the concentration of NLS needed to affect the yeast cell growth without killing them all.

@@ Line 114: / Line 114: @@
 == '''Experiment 2:''' ==
-NLS effect on KO yeast cells growth
+ NLS effect on KO yeast cells growth
 '''1. Introduction:'''