UW-Stout/Hydrogen Peroxide FA22 - Revision history

Teagueb: /* Knockout Experiment */

2022-12-14T16:13:12Z

‎Knockout Experiment

2022-12-14T16:12:50Z

2022-12-14T16:12:11Z

‎Knockout Experiment

2022-12-14T16:07:38Z

‎Knockout Experiment

2022-12-14T16:06:15Z

‎Knockout Experiment

2022-12-14T15:53:38Z

‎Results

2022-12-14T15:52:49Z

‎Results

2022-12-14T15:52:28Z

‎Results

2022-12-14T15:51:53Z

‎Results

2022-12-14T15:48:33Z

‎Results

@@ Line 46: / Line 46: @@
 #Vortex yeast cells briefly to resuspend cells
 #Set up the plate as follows with 50µL of yeast and 50µL of 5000µM hydrogen peroxide
-#Use the following table to set up the wells:                                                        [[Image:KO.jpg|Knockout set up]]
+#Use the following table to set up the wells:                                                                                     [[Image:KO.jpg|Knockout set up]]
 #Set up the plate reader as follows
 ##Temperature: 30°C

@@ Line 46: / Line 46: @@
 #Vortex yeast cells briefly to resuspend cells
 #Set up the plate as follows with 50µL of yeast and 50µL of 5000µM hydrogen peroxide
-#Use the following table to set up the wells:
+#Use the following table to set up the wells:                                                        [[Image:KO.jpg|Knockout set up]]
 #Set up the plate reader as follows
 ##Temperature: 30°C

← Older revision		Revision as of 16:12, 14 December 2022
Line 48:		Line 48:
	#Use the following table to set up the wells:		#Use the following table to set up the wells:
	[[Image:KO.jpg\|Knockout set up]]		[[Image:KO.jpg\|Knockout set up]]
		+	#Set up the plate reader as follows
		+	##Temperature: 30°C
		+	##Mode:Kinetic
		+	##Wavelength: 600 nm
		+	##Interval: 5 minutes
		+	##Total run time: 24 hours
		+	##Shake before read: 30 seconds
		+	#Transfer the assay plate to the read and read for 24 hours

@@ Line 46: / Line 46: @@
 #Vortex yeast cells briefly to resuspend cells
 #Set up the plate as follows with 50µL of yeast and 50µL of 5000µM hydrogen peroxide
-#Use the following table to set up the wells: [[Image:KO.jpg|Knockout set up]]
+#Use the following table to set up the wells:

← Older revision		Revision as of 16:06, 14 December 2022
Line 44:		Line 44:

	==Knockout Experiment==		==Knockout Experiment==
		+	#Vortex yeast cells briefly to resuspend cells
		+	#Set up the plate as follows with 50µL of yeast and 50µL of 5000µM hydrogen peroxide
		+	#Use the following table to set up the wells: [[Image:KO.jpg\|Knockout set up]]

@@ Line 39: / Line 39: @@
 #Transfer the assay plate to the reader and read for 24 hours
 ===Results===
-The following growth curve chart was generated from the data. [[Image:pilot graph.jpg|center|thumb|600px|pilot graph]]
+The following growth curve chart was generated from the data. [[Image:pilot graph.jpg|center|thumb|600px|Pilot experiment graph]]
 The well number corresponds to the concentration as shown in the chart above.
 From the graph, we decided to use the concentration from well A8. This corresponds to the 5000µM hydrogen peroxide solution. This is the concentration we decided to use for the knockout experiment because it demonstrated an effect on the growth of wild type yeast without killing majority of the yeast cells.
 ==Knockout Experiment==