UW-Stout/G418 SP23 - Revision history

Teagueb at 21:46, 5 May 2023

2023-05-05T21:46:59Z

2023-05-04T17:10:12Z

2023-05-04T03:25:42Z

2023-05-04T03:17:42Z

2023-05-04T03:17:15Z

2023-05-04T03:16:14Z

2023-04-20T19:26:56Z

‎G418 Genticin

2023-04-20T19:26:03Z

‎G418 Genticin

2023-04-20T19:25:09Z

‎G418 Genticin

2023-04-20T19:23:41Z

‎G418 Genticin

← Older revision		Revision as of 21:46, 5 May 2023
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	the data collected from the experiment indicated that strain 2&3 were more impacted by geneticin with their respective gene knockout than strain 1 and wild type.		the data collected from the experiment indicated that strain 2&3 were more impacted by geneticin with their respective gene knockout than strain 1 and wild type.
−
−
−	~~'''''Gene:'''''~~
−
−	the gene of focus in this protocol is the YMR144W gene. The gene has no known outcome on phenotype for yeast, through the experiment this was further proven. the use of geneticin on the yeast strain without YMR144W had a similar effect on wild type yeast. In looking into the data for the light capture, wild type yeast and the YMR144W knockout strain had similar growth at the end of the time period given for growth, as seen in figure 1.

← Older revision		Revision as of 17:10, 4 May 2023
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	##Shake before read: 30 seconds		##Shake before read: 30 seconds
	#Transfer the assay plate to the reader and read for 24 hours.		#Transfer the assay plate to the reader and read for 24 hours.
		+
		+	'''''Results:'''''
		+
		+	the data collected from the experiment indicated that strain 2&3 were more impacted by geneticin with their respective gene knockout than strain 1 and wild type.

← Older revision		Revision as of 03:25, 4 May 2023
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	'''''Gene:'''''		'''''Gene:'''''

−	the gene of focus in this protocol is the YMR144W gene. The gene has no known outcome on phenotype for yeast, through the experiment this was further proven. the use of geneticin on the yeast strain without YMR144W had a similar effect on wild type yeast. In looking into the data for the light capture, wild type yeast and the YMR144W knockout strain had similar growth at the end of the time period given for growth, as seen in figure 1.0.	+	the gene of focus in this protocol is the YMR144W gene. The gene has no known outcome on phenotype for yeast, through the experiment this was further proven. the use of geneticin on the yeast strain without YMR144W had a similar effect on wild type yeast. In looking into the data for the light capture, wild type yeast and the YMR144W knockout strain had similar growth at the end of the time period given for growth, as seen in figure 1.

← Older revision		Revision as of 03:17, 4 May 2023
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	'''''Gene:'''''		'''''Gene:'''''
		+
	the gene of focus in this protocol is the YMR144W gene. The gene has no known outcome on phenotype for yeast, through the experiment this was further proven. the use of geneticin on the yeast strain without YMR144W had a similar effect on wild type yeast. In looking into the data for the light capture, wild type yeast and the YMR144W knockout strain had similar growth at the end of the time period given for growth, as seen in figure 1.0.		the gene of focus in this protocol is the YMR144W gene. The gene has no known outcome on phenotype for yeast, through the experiment this was further proven. the use of geneticin on the yeast strain without YMR144W had a similar effect on wild type yeast. In looking into the data for the light capture, wild type yeast and the YMR144W knockout strain had similar growth at the end of the time period given for growth, as seen in figure 1.0.

← Older revision		Revision as of 03:17, 4 May 2023
Line 47:		Line 47:


−	'''''Gene'''''	+	'''''Gene:'''''
	the gene of focus in this protocol is the YMR144W gene. The gene has no known outcome on phenotype for yeast, through the experiment this was further proven. the use of geneticin on the yeast strain without YMR144W had a similar effect on wild type yeast. In looking into the data for the light capture, wild type yeast and the YMR144W knockout strain had similar growth at the end of the time period given for growth, as seen in figure 1.0.		the gene of focus in this protocol is the YMR144W gene. The gene has no known outcome on phenotype for yeast, through the experiment this was further proven. the use of geneticin on the yeast strain without YMR144W had a similar effect on wild type yeast. In looking into the data for the light capture, wild type yeast and the YMR144W knockout strain had similar growth at the end of the time period given for growth, as seen in figure 1.0.

← Older revision		Revision as of 03:16, 4 May 2023
Line 45:		Line 45:
	##Shake before read: 30 seconds		##Shake before read: 30 seconds
	#Transfer the assay plate to the reader and read for 24 hours.		#Transfer the assay plate to the reader and read for 24 hours.
		+
		+
		+	'''''Gene'''''
		+	the gene of focus in this protocol is the YMR144W gene. The gene has no known outcome on phenotype for yeast, through the experiment this was further proven. the use of geneticin on the yeast strain without YMR144W had a similar effect on wild type yeast. In looking into the data for the light capture, wild type yeast and the YMR144W knockout strain had similar growth at the end of the time period given for growth, as seen in figure 1.0.

@@ Line 4: / Line 4: @@
 [[UW-Stout/Knockout_Protocol|knocked out]] three [https://yeastmine.yeastgenome.org/yeastmine/bagDetails.do?scope=all&bagName=Uncharacterized_ORFs genes of unknown function] in a
 [https://www.atcc.org/en/Products/All/200897.aspx BY4735 strain]
-background. The genes are [[YMR144W]], [[SSK1]], and [[YKL162C]]. This page is specifically about screening these knockout strains for phenotypes using G418 Genticin.
+background. This page is specifically about screening these knockout strains for phenotypes using G418 Genticin.
 '''''Materials:'''''
 #'''Wild type yeast''' tube in 2x synthetic complete media at an OD600 of 0.1-0.2
-#Tubes with each modified yeast strain
+#Tubes with each modified yeast strain ([[YMR144W]], [[SSK1]], and [[YKL162C]])
 #100µL of 100mg/mL '''G418 geneticin''' (100 mM solution in water)
 #2mL '''sterile H2O3'''

@@ Line 1: / Line 1: @@
 ===G418 Genticin===
-In the spring of 2023, the Intro Cell and Molecular Biology class at the
+In the spring of 2023, at the
-[https://uwstout.edu University of Wisconsin -- Stout]
+[https://uwstout.edu University of Wisconsin -- Stout], the Intro Cell and Molecular Biology class
 [[UW-Stout/Knockout_Protocol|knocked out]] three [https://yeastmine.yeastgenome.org/yeastmine/bagDetails.do?scope=all&bagName=Uncharacterized_ORFs genes of unknown function] in a
 [https://www.atcc.org/en/Products/All/200897.aspx BY4735 strain]