UW-Stout/Formamide SP22 - Revision history

Teagueb: /* Results */

2022-05-10T03:49:11Z

‎Results

2022-05-10T03:48:52Z

‎Results

2022-05-10T03:46:42Z

‎Results

2022-05-10T03:46:26Z

‎Results

2022-05-10T03:45:59Z

‎Results

2022-05-10T03:44:04Z

‎Materials / Equipment

2022-05-10T03:43:26Z

‎Materials / Equipment

2022-05-10T03:06:30Z

‎Materials / Equipment

2022-05-10T03:05:38Z

‎Procedure

2022-05-10T03:03:40Z

‎Materials / Equipment

@@ Line 98: / Line 98: @@
 [[File:trial11.jpg|300px|]] [[File:trial22.jpg|300px|]] [[File:trial3.jpg|300px|]]
-* The x-axis is in time (min) and the y-axis is growth by concentration
+* The x-axis is in time (min) and the y-axis is optical density at 600
 *Ran three trials, trial one through three is posted up above in picture form as a line graph
 *The dark line in the middle of trail 2 and 3 is a scanning error from the Molecular Devices SpectraMax Plus 384 Microplate Reader
 *Computed doubling times from the average times of the three trials for further analyzation

@@ Line 50: / Line 50: @@
 [[File:pilot1.jpg|300px|]]
-* The x-axis is in time (min) and the y-axis is growth by concentration
+* The x-axis is in time (min) and the y-axis is optical density at 600
 *The x-axis corresponds to the concentrations listed up above under the title "formamide concentrations"
 *0% is an experimental error- since it has a concentration of 0% formamide anyway, it was left in the final graph
@@ Line 58: / Line 58: @@
-* The x-axis is in time (min) and the y-axis is growth by concentration
+* The x-axis is in time (min) and the y-axis is optical density at 600
 *The 3% formamide used in the pilot experiment and represented as the light blue color (in the top-most picture) was chosen to be used on the transformed yeast cells
 **This was deduced due to the position of the yeast cells under 3% formamide stress

@@ Line 98: / Line 98: @@
 [[File:trial11.jpg|300px|]] [[File:trial22.jpg|300px|]] [[File:trial3.jpg|300px|]]
+* The x-axis is in time (min) and the y-axis is growth by concentration
 *Ran three trials, trial one through three is posted up above in picture form as a line graph
 *The dark line in the middle of trail 2 and 3 is a scanning error from the Molecular Devices SpectraMax Plus 384 Microplate Reader
 *Computed doubling times from the average times of the three trials for further analyzation

@@ Line 50: / Line 50: @@
 [[File:pilot1.jpg|300px|]]
+* The x-axis is in time (min) and the y-axis is growth by concentration
 *The x-axis corresponds to the concentrations listed up above under the title "formamide concentrations"
 *0% is an experimental error- since it has a concentration of 0% formamide anyway, it was left in the final graph

← Older revision		Revision as of 03:45, 10 May 2022
Line 58:		Line 58:


		+	* The x-axis is in time (min) and the y-axis is growth by concentration
	*The 3% formamide used in the pilot experiment and represented as the light blue color (in the top-most picture) was chosen to be used on the transformed yeast cells		*The 3% formamide used in the pilot experiment and represented as the light blue color (in the top-most picture) was chosen to be used on the transformed yeast cells
	**This was deduced due to the position of the yeast cells under 3% formamide stress		**This was deduced due to the position of the yeast cells under 3% formamide stress

@@ Line 71: / Line 71: @@
 *Knock out yeast cells
 *Molecular Devices SpectraMax Plus 384 Microplate Reader
-*Micropipet 100ul
+*Micropipet (100ul)
 *Micropipet tips

@@ Line 63: / Line 63: @@
 ===Materials / Equipment===
-*Neat formamide, 18ul
+*Neat formamide, '''18ul'''
-*Sterile water, 423ul
+*Sterile water, '''423ul'''
-*0.2ml flat cap PCR tube 10
+*0.2ml flat cap PCR tube, '''10 tubes'''
 *Well plate
 *Knock out yeast cells

@@ Line 31: / Line 31: @@
 # Obtain the neat formamide (refrigerated) and sterile water
 # Obtain and label 12 PCR tubes 1-12
-# Obtain one more PCR tube to transfer 33ul of neat formamide inside, can put excess in
+# Obtain one more PCR tube to transfer 33ul of neat formamide inside, can put excess formamide in
 # Using the 13th PCR tube, pipet appropriate amount of formamide and sterile water into the labeled PCR tubes according to number
-# In a sterile environment, pipet the liquid (50ul) from the PCR tube into the well cell
+# In a sterile environment, pipet the solution (50ul) from the PCR tube into the well cell
-# Vortex the yeast culture briefly to resuspend the yeast cells, and then pipet 50ul of the wild yeast cells into each well in addition to the formamide
+# Vortex the yeast culture briefly to resuspend the yeast cells, and then pipet 50ul of the wild yeast cells into each well in addition to the formamide-water solution
 # Set up the plate reader as follows:
 ## Temperature: 30 degrees Celsius

@@ Line 5: / Line 5: @@
 ===Materials / Equipment===
-*Neat Formamide, """33ul"""
+*Neat Formamide, '''33ul'''
-*Sterile water, """567ul"""
+*Sterile water, '''567ul'''
-*0.2ml flat cap PCR tube, """13 tubes"""
+*0.2ml flat cap PCR tube, '''13 tubes'''
 *Corning COSTAR 96-well clear flat-bottom assay plate
-*Wild-type yeast in 2x synthetic complete media at an OD600 of 0.1-0.2, """600ul"""
+*Wild-type yeast in 2x synthetic complete media at an OD600 of 0.1-0.2, '''600ul'''
 *Molecular Devices SpectraMax Plus 384 Microplate Reader
 *Micropipet 100ul