UW-Stout/Ethanol FA22 - Revision history

Teagueb: /* Materials */

2022-12-17T16:33:56Z

‎Materials

2022-12-17T16:32:08Z

‎Materials

2022-12-13T21:18:17Z

‎Analysis

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‎Procedure

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‎Analysis

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‎Analysis

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‎Analysis

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‎Analysis

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‎Analysis

2022-12-13T20:53:12Z

‎Analysis

@@ Line 53: / Line 53: @@
 *Sterile water
 '''*[[BY-BY4735]] (wild type)'''
-*[[YJL133C-A]] yeast knockout strand(well 2)
+*[[YJL133C-A]] yeast knockout strand (well 2)
-*[[YER186C]] yeast knockout strand(well 3)
+*[[YER186C]] yeast knockout strand (well 3)
-*[[YER076C]] yeast knockout strand(well 4)
+*[[YER076C]] yeast knockout strand (well 4)
-*[[YHR033W]] yeast knockout strand(well 5)
+*[[YHR033W]] yeast knockout strand (well 5)
-*[[YCR095C]] yeast knockout strand(well 6)
+*[[YCR095C]] yeast knockout strand (well 6)
 ===Procedure===

@@ Line 53: / Line 53: @@
 *Sterile water
 '''*[[BY-BY4735]] (wild type)'''
-*[[YJL133C-A]] yeast knockout strand
+*[[YJL133C-A]] yeast knockout strand(well 2)
-*[[YER186C]] yeast knockout strand
+*[[YER186C]] yeast knockout strand(well 3)
-*[[YER076C]] yeast knockout strand
+*[[YER076C]] yeast knockout strand(well 4)
-*[[YHR033W]] yeast knockout strand
+*[[YHR033W]] yeast knockout strand(well 5)
-*[[YCR095C]] yeast knockout strand
+*[[YCR095C]] yeast knockout strand(well 6)
 ===Procedure===

@@ Line 41: / Line 41: @@
 Well 11 had a slow growth and the curve plateaued out just under 0.4 OD600.
 Well 12 also had a slow growth and the curve is concave down parabola shape that peaks at 0.37 OD600.
-Well 11 and 12 were our outliers because it did not fit into our expected curve.
+Well 11 and 12 were our outliers because it did not fit into our expected curve. Overall we observed how Ethanol affected yeast. We found a concentration of Ethanol that made the yeast cells stressed out while still growing. We concluded that the appropriate concentration of Ethanol is 1M.
 ==Knockout Yeast Ethanol Stress Experiment==

@@ Line 67: / Line 67: @@
 #Vortex yeast culture for about 5 seconds before adding to the wells
 #Add 50 µl of wild yeast culture and 50 ul of ethanol solution into well 1
-#Add 50 µl of knockout strain yeast culture and 50 ul of ethanol solution #1 into well 2
+#Add 50 µl of knockout strain yeast culture and 50 µlof ethanol solution #1 into well 2
-#Add 50 µl of knockout strain yeast culture and 50 ul of ethanol solution #2 into well 3
+#Add 50 µl of knockout strain yeast culture and 50 µlof ethanol solution #2 into well 3
-#Add 50 µl of knockout strain yeast culture and 50 ul of ethanol solution #3 into well 4
+#Add 50 µl of knockout strain yeast culture and 50 µl of ethanol solution #3 into well 4
-#Add 50 µl of knockout strain yeast culture and 50 ul of ethanol solution #4 into well 5
+#Add 50 µl of knockout strain yeast culture and 50 µl of ethanol solution #4 into well 5
-#Add 50 µl of knockout strain yeast culture and 50 ul of ethanol solution #5 into well 6
+#Add 50 µl of knockout strain yeast culture and 50 µl of ethanol solution #5 into well 6
 #Put your well clear flat-bottom assay plate into the incubator and let sit
 #Wait and observe the growth

@@ Line 82: / Line 82: @@
 -Well 2 line lies on the middle of the overall growth curve. Doubling time is 67.78
--Well 3 is our outlier. We believe that this knockout strand had a slower growth because the missing gene affect that growth of the cell. We think Ethanol did not cause the large decrease in growth that we observed. The doubling time is 189.60
+-Well 3 is the outlier. The Ethanol dramatically decreased cell growth. The doubling time is 189.60
 -Well 4 had the largest cell growth. Doubling time is 71.60
@@ Line 89: / Line 89: @@
 -Well 6 cell growth started off at a higher growth rate but decreased at an earlier rate. The doubling time is 60.40

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 [[Image:Best Ethanol KO graph.png|Graph|center|1000px]]
 ===Analysis===
-*Well 1 with the wild type had a steady growth throughout the experiment. Doubling time is 71.76
+-Well 1 with the wild type had a steady growth throughout the experiment. Doubling time is 71.76
-*Well 2 line lies on the middle of the overall growth curve.
+-Well 2 line lies on the middle of the overall growth curve.
-*Well 3 is our outlier. We believe that this knockout strand had a slower growth because the missing gene affect that growth of the cell. We think Ethanol did not cause the large decrease in growth that we observed.
+-Well 3 is our outlier. We believe that this knockout strand had a slower growth because the missing gene affect that growth of the cell. We think Ethanol did not cause the large decrease in growth that we observed.
-*Well 4 had the largest cell growth.
+-Well 4 had the largest cell growth.
-*Well 5 is slightly above well 2 but has a similar curve.
+-Well 5 is slightly above well 2 but has a similar curve.
 -Well 6 cell growth started off at a higher growth rate but decreased at an earlier rate.

← Older revision		Revision as of 20:53, 13 December 2022
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	[[Image:Best Ethanol KO graph.png\|Graph\|center\|1000px]]		[[Image:Best Ethanol KO graph.png\|Graph\|center\|1000px]]
	===Analysis===		===Analysis===
		+	Well 1 with the wild type had a steady growth throughout the experiment.
		+	Well 2 line lies on the middle of the overall growth curve.
		+	Well 3 is our outlier. We believe that this knockout strand had a slower growth because the missing gene affect that growth of the cell. We think Ethanol did not cause the large decrease in growth that we observed.
		+	Well 4 had the largest cell growth.
		+	Well 5 is slightly above well 2 but has a similar curve.
		+	Well 6 cell growth started off at a higher growth rate but decreased at an earlier rate.