UW-Stout/Bud Scars FA22 - Revision history

Teagueb at 02:15, 21 December 2022

2022-12-21T02:15:18Z

Teagueb at 02:13, 21 December 2022

2022-12-21T02:13:58Z

Teagueb at 00:55, 21 December 2022

2022-12-21T00:55:22Z

Teagueb at 00:54, 21 December 2022

2022-12-21T00:54:23Z

Teagueb at 00:36, 21 December 2022

2022-12-21T00:36:10Z

Teagueb at 00:23, 21 December 2022

2022-12-21T00:23:35Z

Teagueb at 00:20, 21 December 2022

2022-12-21T00:20:51Z

Teagueb at 00:13, 21 December 2022

2022-12-21T00:13:21Z

Teagueb at 00:11, 21 December 2022

2022-12-21T00:11:32Z

Teagueb at 00:09, 21 December 2022

2022-12-21T00:09:14Z

@@ Line 21: / Line 21: @@
 #Pellet, remove liquid and suspend in 10 ul of calcofluor
 #Incubate at room temperature for 5 minutes
-#Centrifuge (13k for 60 seconds) remove liquid
+#Centrifuge (13k for 60 seconds) and then remove liquid
 #Add 1 ml of water, vortex briefly, centrifuge (13k for 60 seconds), remove liquid
 #Resuspend pellet in 50 ul of water

@@ Line 6: / Line 6: @@
 *Knockout yeast culture
 *1.7ml microcentrifuge tube (1 for each culture)
-*Calcofluor White Stain Kit Reagent B- Calcofluor White (1.0g), Evans Blue Dye (0.4g), and demineralized Water (1000.0ml) (10 ul for each culture)
+*Calcofluor White Stain Kit Reagent B- Calcofluor White (1.0g), Evans Blue Dye (0.4g), and demineralized Water (1000.0ml), (10 ul for each culture)
 *Glass Microscope Slide
 *Coverslip

@@ Line 29: / Line 29: @@
 -For this experiment we saved pictures of the yeast and counted the yeast until we had counted 100 cells and we recorded the amount of bud scars present. We had two people count the cells and if the numbers matched then we recorded that data and if the numbers did not match then we recounted the cells. No cells that were hard to see or oddly shaped were counted due to them possibly adding inaccuracies to the data
 -We ended up getting an average of 2.46 bud scars per cell with the wild yeast strain (BY4735).
 [[File:Yeast_wild(2)_1_9.png]]

@@ Line 1: / Line 1: @@
+'''Introduction'''
 '''Materials'''
@@ Line 28: / Line 29: @@
 -For this experiment we saved pictures of the yeast and counted the yeast until we had counted 100 cells and we recorded the amount of bud scars present. We had two people count the cells and if the numbers matched then we recorded that data and if the numbers did not match then we recounted the cells. No cells that were hard to see or oddly shaped were counted due to them possibly adding inaccuracies to the data
 [[File:Yeast_wild(2)_1_9.png]]
 [[File:Yeast_wild(2)_2_9.png]]

@@ Line 29: / Line 29: @@
 -For this experiment we saved pictures of the yeast and counted the yeast until we had counted 100 cells and we recorded the amount of bud scars present. We had two people count the cells and if the numbers matched then we recorded that data and if the numbers did not match then we recounted the cells. No cells that were hard to see or oddly shaped were counted due to them possibly adding inaccuracies to the data
 [[File:Yeast_wild(2)_1_9.png]]
+[[File:Yeast_wild(2)_2_9.png]]
+[[File:Yeast_wild(2)_3_11.png]]
+[[File:Yeast_wild(2)_4_6.png]]
-On average, yeast 1 (YJL133C-A) had 1.06 bud scars per yeast cell. While on average, wild yeast (BY4735) had 2.46 bud scars per yeast cell. This particular knockout is more resistant than our wild-type yeast.
+[[File:Yeast_wild(2)_5_13.png]]
+[[File:Yeast_wild(2)_6_7.png]]
-On average, yeast 2 (YER186C) had 2.82 bud scars per yeast cell. While on average, wild yeast (BY4735) had 2.46 bud scars per yeast cell. This particular knockout is slightly more sensitive than our wild-type yeast.
+[[File:Yeast_wild(2)_7_15.png]]
+[[File:Yeast_wild(2)_8_7.png]]
-On average, yeast 3 (YER076C) had 1.46 bud scars per yeast cell. While on average, wild yeast (BY4735) had 2.46 bud scars per yeast cell. This particular knockout is more resistant than our wild-type yeast.
+[[File:Yeast_wild(2)_9_9.png]]
+[[File:Yeast_wild(2)_10_7.png]]
-On average, yeast 4 (YHR033W) had 1.03 bud scars per yeast cell. While on average, wild yeast (BY4735) had 2.46 bud scars per yeast cell. This particular knockout is more resistant than our wild-type yeast.
+[[File:Yeast_wild(2)_11_13.png]]

← Older revision		Revision as of 00:23, 21 December 2022
Line 29:		Line 29:
	-For this experiment we saved pictures of the yeast and counted the yeast until we had counted 100 cells and we recorded the amount of bud scars present. We had two people count the cells and if the numbers matched then we recorded that data and if the numbers did not match then we recounted the cells. No cells that were hard to see or oddly shaped were counted due to them possibly adding inaccuracies to the data		-For this experiment we saved pictures of the yeast and counted the yeast until we had counted 100 cells and we recorded the amount of bud scars present. We had two people count the cells and if the numbers matched then we recorded that data and if the numbers did not match then we recounted the cells. No cells that were hard to see or oddly shaped were counted due to them possibly adding inaccuracies to the data
	[[File:Yeast_wild(2)_1_9.png]]		[[File:Yeast_wild(2)_1_9.png]]
		+
		+
		+
		+	On average, yeast 1 (YJL133C-A) had 1.06 bud scars per yeast cell. While on average, wild yeast (BY4735) had 2.46 bud scars per yeast cell. This particular knockout is more resistant than our wild-type yeast.
		+
		+	On average, yeast 2 (YER186C) had 2.82 bud scars per yeast cell. While on average, wild yeast (BY4735) had 2.46 bud scars per yeast cell. This particular knockout is slightly more sensitive than our wild-type yeast.
		+
		+	On average, yeast 3 (YER076C) had 1.46 bud scars per yeast cell. While on average, wild yeast (BY4735) had 2.46 bud scars per yeast cell. This particular knockout is more resistant than our wild-type yeast.
		+
		+	On average, yeast 4 (YHR033W) had 1.03 bud scars per yeast cell. While on average, wild yeast (BY4735) had 2.46 bud scars per yeast cell. This particular knockout is more resistant than our wild-type yeast.
		+
		+	On average, yeast 5 (YCR095C) had 2.13 bud scars per yeast cell. While on average, wild yeast (BY4735) had 2.46 bud scars per yeast cell. This particular knockout is more resistant than our wild-type yeast.

@@ Line 26: / Line 26: @@
 #Put a paper towel below and above and cover with a heavy object for 10 minutes
 #Look at the cells under a microscope and observe the number of bud scars on each cell. View 100 different cells
 [[File:Yeast_wild(2)_1_9.png]]

← Older revision		Revision as of 00:11, 21 December 2022
Line 26:		Line 26:
	#Put a paper towel below and above and cover with a heavy object for 10 minutes		#Put a paper towel below and above and cover with a heavy object for 10 minutes
	#Look at the cells under a microscope and observe the number of bud scars on each cell. View 100 different cells		#Look at the cells under a microscope and observe the number of bud scars on each cell. View 100 different cells
		+	[[File:Yeast_wild(2)_1_9]]

@@ Line 9: / Line 9: @@
 *Coverslip
 *Microscope
-*Distilled Water ()
+*Distilled Water (3,050 ul)
 '''Procedure'''