UW-Stout/Acids SP23 - Revision history

Teagueb: /* Results */

2023-05-05T18:51:15Z

‎Results

2023-05-05T18:50:20Z

2023-05-05T18:46:54Z

2023-05-05T18:35:20Z

2023-05-05T17:01:12Z

2023-05-05T16:54:10Z

2023-05-03T02:38:00Z

2023-05-03T02:36:45Z

2023-05-03T02:36:30Z

2023-05-03T02:36:06Z

@@ Line 38: / Line 38: @@
 [[Image:AceticAcidGraph2.jpg]]
 ===Results===
-Our results show that acid became more diluted, the yeast had more successful growth curve. Since we were looking for an acid concentration that would stress yeast growth, but still allow there to be growth, we decided to go with a concentration in the middle. We settled on concentration 6 for our final experiment.
+Our results show that acid became more diluted, the yeast had a more successful growth curve. Since we were looking for an acid concentration that would stress yeast growth, but still allow there to be growth, we decided to go with a concentration in the middle. We settled on concentration 6 for our final experiment.
 =='''Final Experiment'''==
 ===Introduction===

@@ Line 38: / Line 38: @@
 [[Image:AceticAcidGraph2.jpg]]
 ===Results===
-Our results show that acid became more diluted, the yeast had more successful growth curve.
+Our results show that acid became more diluted, the yeast had more successful growth curve. Since we were looking for an acid concentration that would stress yeast growth, but still allow there to be growth, we decided to go with a concentration in the middle. We settled on concentration 6 for our final experiment.
 =='''Final Experiment'''==
 ===Introduction===

@@ Line 36: / Line 36: @@
 #Transfer assay plate to reader and read for 24 hours
 ===Data===
-[[Image:AceticAcidGraph.jpg]]
+[[Image:AceticAcidGraph2.jpg]]
 ===Results===
 =='''Final Experiment'''==
 ===Introduction===

@@ Line 36: / Line 36: @@
 #Transfer assay plate to reader and read for 24 hours
 ===Data===
 ===Results===
 =='''Final Experiment'''==

@@ Line 48: / Line 48: @@
 *Acetic acid concentration 6
 *Corning COSTAR 96-well clear flat-bottom assay plate
 ===Procedure===

@@ Line 2: / Line 2: @@
 ===Introduction===
 We are looking to find a pH that puts yeast under stress. Our end goal is to be able to test different knockout yeast strains against an acidic medium, but to do this we first need to find a pH that allows the yeast to grow, but puts some strain on the growth curve. In this protocol, we are testing wild yeast strains against different concentration of acetic acid. By analyzing the growth curves, we will be able to find a concentration that will stress the yeast growth.
-=='''Materials'''==
+===Materials===
 *Wild-type yeast in 2x synthetic complete media at an OD600 of 0.1-0.2
 *17.4 M Glacial Acetic Acid
@@ Line 11: / Line 11: @@
 *1.7 mL microcentrifuge tubes
 *Disposable Gloves
-=='''Procedure'''==
+===Procedure===
 #Using 17.4 M Glacial Acetic Acid, create a diluted stock solution
 #:*Use gloves when working with concentrated acid
@@ Line 35: / Line 35: @@
 #:*Shake Before Read - 30 seconds
 #Transfer assay plate to reader and read for 24 hours
-=='''Data'''==
+===Data===

@@ Line 1: / Line 1: @@
 =='''Pilot Experiment'''==
-=='''Introduction'''==
+==='''Introduction'''===
 We are looking to find a pH that puts yeast under stress. Our end goal is to be able to test different knockout yeast strains against an acidic medium, but to do this we first need to find a pH that allows the yeast to grow, but puts some strain on the growth curve. In this protocol, we are testing wild yeast strains against different concentration of acetic acid. By analyzing the growth curves, we will be able to find a concentration that will stress the yeast growth.
 =='''Materials'''==