Commonly used strains - Revision history

Nash: /* Sigma1278b */

2025-05-06T23:06:27Z

‎Sigma1278b

2025-05-06T23:05:51Z

‎Sigma1278b

2025-05-06T23:02:36Z

‎Sigma1278b

2025-05-06T23:02:00Z

‎Sigma1278b

2024-08-13T19:32:49Z

‎W1588-4C

2024-02-14T20:42:31Z

‎BY4743

2023-12-14T23:03:34Z

‎DBY1091

2023-12-14T23:03:14Z

‎JT150

2023-12-14T23:03:02Z

‎TB123

2023-12-14T23:02:54Z

‎TB50

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 Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found in [http://www.yeastgenome.org/reference/S000133862/overview Dowell et al.] (2010).
-The sequence of Ena1p in Σ1278b contains 14 amino acid level changes compared to S288C. 11 are shared with Ena1p of the CEN.PK, SK1 and Y55 strains (i.e., V101, G102, G106, D191, T204, R497, A753, N882, L1045, and I1085). Only C556, F860 and G1070 are unique to Ena1p of Σ1278b. F860 results in non-functionality of the protein that can be restored by replacement with Serine as found in S288C, SK1, CEN.PK, Y55 and RM11 as described in [https://www.yeastgenome.org/reference/S100000488 Engelberg et al.] (2025).
+The sequence of Ena1p in Σ1278b contains 14 amino acid level changes compared to S288C. 11 are shared with Ena1p of CEN.PK, SK1 and Y55 strains (i.e., V101, G102, G106, D191, T204, R497, A753, N882, L1045, and I1085). Only C556, F860 and G1070 are unique to Ena1p of Σ1278b. F860 results in non-functionality of the protein that can be restored by replacement with Serine as found in S288C, SK1, CEN.PK, Y55 and RM11 as described in [https://www.yeastgenome.org/reference/S100000488 Engelberg et al.] (2025).
 =SK1=

@@ Line 226: / Line 226: @@
 Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found in [http://www.yeastgenome.org/reference/S000133862/overview Dowell et al.] (2010).
-The sequence of Ena1p in Σ1278b contains 14 amino acid level changes compared to S288C. 11 are shared with Ena1p of the CEN.PK, SK1 and Y55 strains (i.e., V101, G102, G106, D191, T204, R497, A753, N882, L1045, and I1085). Only C556, F860 and G1070 are unique to Ena1p of Σ1278b. F860 results in non-functionality of the protein that can be restored by replacement with Serine as found in S288C, SK1, CEN.PK, Y55 and RM11 as described in [https://www.yeastgenome.org/reference/SGD:S100000488 Engelberg et al.] (2025).
+The sequence of Ena1p in Σ1278b contains 14 amino acid level changes compared to S288C. 11 are shared with Ena1p of the CEN.PK, SK1 and Y55 strains (i.e., V101, G102, G106, D191, T204, R497, A753, N882, L1045, and I1085). Only C556, F860 and G1070 are unique to Ena1p of Σ1278b. F860 results in non-functionality of the protein that can be restored by replacement with Serine as found in S288C, SK1, CEN.PK, Y55 and RM11 as described in [https://www.yeastgenome.org/reference/S100000488 Engelberg et al.] (2025).
 =SK1=

@@ Line 226: / Line 226: @@
 Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found in [http://www.yeastgenome.org/reference/S000133862/overview Dowell et al.] (2010).
-The sequence of Ena1p in Σ1278b contains 14 amino acid level changes compared to S288C. 11 are shared with Ena1p of the CEN.PK, SK1 and Y55 strains (i.e., V101, G102, G106, D191, T204, R497, A753, N882, L1045, and I1085). Only C556, F860 and G1070 are unique to Ena1p of Σ1278b. F860 results in non-functionality of the protein that can be restored by replacement with Serine as found in S288C, SK1, CEN.PK, Y55 and RM11[https://www.yeastgenome.org/reference/SGD:S100000488 Engelberg et al.] (2025).
+The sequence of Ena1p in Σ1278b contains 14 amino acid level changes compared to S288C. 11 are shared with Ena1p of the CEN.PK, SK1 and Y55 strains (i.e., V101, G102, G106, D191, T204, R497, A753, N882, L1045, and I1085). Only C556, F860 and G1070 are unique to Ena1p of Σ1278b. F860 results in non-functionality of the protein that can be restored by replacement with Serine as found in S288C, SK1, CEN.PK, Y55 and RM11 as described in [https://www.yeastgenome.org/reference/SGD:S100000488 Engelberg et al.] (2025).
 =SK1=

← Older revision		Revision as of 23:02, 6 May 2025
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	Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found in [http://www.yeastgenome.org/reference/S000133862/overview Dowell et al.] (2010).		Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found in [http://www.yeastgenome.org/reference/S000133862/overview Dowell et al.] (2010).
		+
		+	The sequence of Ena1p in Σ1278b contains 14 amino acid level changes compared to S288C. 11 are shared with Ena1p of the CEN.PK, SK1 and Y55 strains (i.e., V101, G102, G106, D191, T204, R497, A753, N882, L1045, and I1085). Only C556, F860 and G1070 are unique to Ena1p of Σ1278b. F860 results in non-functionality of the protein that can be restored by replacement with Serine as found in S288C, SK1, CEN.PK, Y55 and RM11[https://www.yeastgenome.org/reference/SGD:S100000488 Engelberg et al.] (2025).

	=SK1=		=SK1=

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 RAD5 derivative of W303
-'''Genotype''': ''MAT''a ''ade2-1 can1-100 ura3-1 his3-11,15 leu2-3,112 trp1-1''
+'''Genotype''': ''MAT''a ''ade2-1 can1-100 ura3-1 his3-11,15 leu2-3,112 trp1-1 RAD5''
 '''Reference''': Dhingra N, et al. (2019) Replication protein A (RPA) sumoylation positively influences the DNA damage checkpoint response in yeast. J Biol Chem 294(8):2690-2699 PMID:30591583

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 '''Sources:''' [http://www.atcc.org/products/all/201390.aspx ATCC:201390]
 ==CKY8==
 '''Genotype:''' ''MAT''&#x3B1; ''ura3-52 leu2-3,112''

← Older revision		Revision as of 23:03, 14 December 2023
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	'''References:''' [https://journals.asm.org/doi/epdf/10.1128/mcb.13.8.4806-4813.1993 Costanzo & Fox] (1993) MCB 13(8):4806-13 \| [https://www.pnas.org/content/pnas/93/11/5253.full.pdf Steele et al.] (1996) PNAS 93:5253-7		'''References:''' [https://journals.asm.org/doi/epdf/10.1128/mcb.13.8.4806-4813.1993 Costanzo & Fox] (1993) MCB 13(8):4806-13 \| [https://www.pnas.org/content/pnas/93/11/5253.full.pdf Steele et al.] (1996) PNAS 93:5253-7
−
−	~~==DBY1091==~~
−	~~'''Genotype:''' ''MAT''a/α ''+/his4'' ''+/can1'' ''+/ade2-101'' ''ura3-52/ura3-52''~~

	==DC5==		==DC5==

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 '''Sources:''' [http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/fy1679.html EUROSCARF:10000D]
 ==NY13==
 isogenic with S288C PMID:24476960

← Older revision		Revision as of 23:03, 14 December 2023
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	'''References:''' [http://www.yeastgenome.org/reference/S000054286/overview Heitman et al.] (1991a) Science 253(5022):905-9 and [http://www.yeastgenome.org/reference/S000054822/overview Heitman et al.] (1991b) Proc Natl Acad Sci U S A 88(5):1948-52		'''References:''' [http://www.yeastgenome.org/reference/S000054286/overview Heitman et al.] (1991a) Science 253(5022):905-9 and [http://www.yeastgenome.org/reference/S000054822/overview Heitman et al.] (1991b) Proc Natl Acad Sci U S A 88(5):1948-52
−	~~==TB123==~~
−	~~'''Genotype:''' JK9-3da ''MAT''a ''leu2-3,112 ura3-52 rme1 trp1 his4 GAL+ HMLa, GLN3-Myc<sup>13</sup>[KanMX]''~~
−
	=RM11-1a=		=RM11-1a=

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 '''References:''' [http://www.yeastgenome.org/reference/S000054286/overview Heitman et al.] (1991a) Science 253(5022):905-9 and [http://www.yeastgenome.org/reference/S000054822/overview Heitman et al.] (1991b) Proc Natl Acad Sci U S A 88(5):1948-52
 ==TB123==
 '''Genotype:''' JK9-3da  ''MAT''a ''leu2-3,112 ura3-52 rme1 trp1 his4 GAL+ HMLa, GLN3-Myc<sup>13</sup>[KanMX]''